Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,196,292 G→A 7.5% intergenic (+134/+235) PP_5618 → / ← PP_5619 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,196,2920GA 7.5% 148.5 / 2.7 53intergenic (+134/+235)PP_5618/PP_5619Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base A (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

AGGGCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAG  >  NC_002947/4196147‑4196439
                                                                                                                                                 |                                                                                                                                                   
aGGGCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGgtg                                                                                                                                                  >  1:113291/1‑149 (MQ=255)
  ggCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTc                                                                                                                                                <  2:113291/149‑1 (MQ=255)
    ccTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGa                                                                                                                                              >  5:345848/1‑149 (MQ=255)
     cTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGAc                                                                                                                                             <  1:322656/149‑1 (MQ=255)
     cTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGAc                                                                                                                                             <  8:221941/149‑1 (MQ=255)
                   ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCg                                                                                                                               >  5:18823/1‑149 (MQ=255)
                   ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCg                                                                                                                                       <  4:145415/141‑1 (MQ=255)
                   ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCg                                                                                                                                       >  3:145415/1‑141 (MQ=255)
                     aCTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                             <  4:118433/149‑1 (MQ=255)
                      cTGCTCGCTTCTTCTCTACGCGTGGAGTGTACTGGCGCGACAAGCTCGCATGAGATCGCGTAGCAGATCTTGCTAAGGAGTGCGTGCGCTATGGTGCCGTCTGTCTTTAACCGAGCCGTCTACGGTGTCGACGTGTCTGCCCTCCCGGc                                                                                                                            <  2:220815/149‑1 (MQ=255)
                         cTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGc                                                                                                                         >  4:219199/1‑149 (MQ=255)
                            gCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCAt                                                                                                                      >  2:25769/1‑149 (MQ=255)
                                cTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGt                                                                                                                  >  3:229443/1‑149 (MQ=255)
                                   ctctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCAc                                                                                                               <  6:106521/149‑1 (MQ=255)
                                    tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCATCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACg                                                                                                              >  8:165894/1‑149 (MQ=255)
                                                gggTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGACATCAGTCACGCTTGCGCGccc                                                                                                   >  2:35236/1‑148 (MQ=255)
                                                    aaTGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACtt                                                                                              >  7:1592/1‑149 (MQ=255)
                                                      tGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGc                                                                                            <  3:219199/149‑1 (MQ=255)
                                                        gCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGcc                                                                                           <  4:229443/148‑1 (MQ=255)
                                                        gCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCt                                                                                          <  2:248648/149‑1 (MQ=255)
                                                                aGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGa                                                                                  <  1:25769/149‑1 (MQ=255)
                                                                 gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgc                                                                                        <  1:346623/142‑1 (MQ=255)
                                                                 gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgc                                                                                        >  2:346623/1‑142 (MQ=255)
                                                                  cTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgcag                                                                                      >  8:236819/1‑143 (MQ=255)
                                                                  cTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgcag                                                                                      <  7:236819/143‑1 (MQ=255)
                                                                      cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                            <  6:18823/149‑1 (MQ=255)
                                                                       aTGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                            >  8:32490/1‑148 (MQ=255)
                                                                        tGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTg                                                                           >  8:143145/1‑148 (MQ=255)
                                                                          agaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGaa                                                                         <  8:1592/148‑1 (MQ=255)
                                                                           gaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACa                                                                       >  8:176200/1‑149 (MQ=255)
                                                                             tCGCCTAGCAGATCTTGCTAAGTACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGTCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACAt                                                                      <  6:345848/148‑1 (MQ=255)
                                                                               gCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGct                                                                   <  7:143145/149‑1 (MQ=255)
                                                                                 cTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGctct                                                                 >  3:83823/1‑149 (MQ=255)
                                                                                  tAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTg                                                                >  7:110152/1‑149 (MQ=255)
                                                                                                     cTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATtg                                             >  8:30232/1‑149 (MQ=255)
                                                                                                             ccTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                     <  8:110152/149‑1 (MQ=255)
                                                                                                              cTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGCACATGCTCTGTTCGACCGCTGATGGATTGCTGCGTTTg                                    >  4:248968/1‑149 (MQ=255)
                                                                                                               tATGGGCGCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                     <  1:83357/147‑1 (MQ=255)
                                                                                                               tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                     >  2:83357/1‑147 (MQ=255)
                                                                                                                 tGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTggg                                  <  7:30232/148‑1 (MQ=255)
                                                                                                                   ggCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTa                                <  1:338832/148‑1 (MQ=255)
                                                                                                                           tGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                  <  1:326000/122‑1 (MQ=255)
                                                                                                                           tGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                  >  2:326000/1‑122 (MQ=255)
                                                                                                                             tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGaa                      <  7:32490/148‑1 (MQ=255)
                                                                                                                               gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAAc                     >  1:164100/1‑147 (MQ=255)
                                                                                                                                   aCCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACa               <  4:83823/149‑1 (MQ=255)
                                                                                                                                    ccGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAg              <  7:176200/149‑1 (MQ=255)
                                                                                                                                    ccGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAg              >  8:172656/1‑149 (MQ=255)
                                                                                                                                     cGAGACGTCGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGc             >  6:31283/1‑149 (MQ=255)
                                                                                                                                     cGAGACGTCGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGc             <  5:31283/149‑1 (MQ=255)
                                                                                                                                       aGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCg            >  1:10787/1‑148 (MQ=255)
                                                                                                                                               aCGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGTTATGACTCAGAACGAGACAGCGAAGAAGCTa   <  1:35236/149‑1 (MQ=255)
                                                                                                                                                cGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAg  <  5:114720/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGGGCCTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAG  >  NC_002947/4196147‑4196439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑