Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,196,302 T→G 7.2% intergenic (+144/+225) PP_5618 → / ← PP_5619 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,196,3020TG 7.2% 164.9 / 4.6 56intergenic (+144/+225)PP_5618/PP_5619Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base G (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

CCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGCC  >  NC_002947/4196166‑4196446
                                                                                                                                        |                                                                                                                                                
ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCg                                                                                                                                      >  5:18823/1‑149 (MQ=255)
ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCg                                                                                                                                              >  3:145415/1‑141 (MQ=255)
ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCg                                                                                                                                              <  4:145415/141‑1 (MQ=255)
  aCTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                                    <  4:118433/149‑1 (MQ=255)
   cTGCTCGCTTCTTCTCTACGCGTGGAGTGTACTGGCGCGACAAGCTCGCATGAGATCGCGTAGCAGATCTTGCTAAGGAGTGCGTGCGCTATGGTGCCGTCTGTCTTTAACCGAGCCGTCTACGGTGTCGACGTGTCTGCCCTCCCGGc                                                                                                                                   <  2:220815/149‑1 (MQ=255)
      cTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGc                                                                                                                                >  4:219199/1‑149 (MQ=255)
         gCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCAt                                                                                                                             >  2:25769/1‑149 (MQ=255)
             cTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGt                                                                                                                         >  3:229443/1‑149 (MQ=255)
                ctctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCAc                                                                                                                      <  6:106521/149‑1 (MQ=255)
                 tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCATCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACg                                                                                                                     >  8:165894/1‑149 (MQ=255)
                             gggTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGACATCAGTCACGCTTGCGCGccc                                                                                                          >  2:35236/1‑148 (MQ=255)
                                 aaTGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACtt                                                                                                     >  7:1592/1‑149 (MQ=255)
                                   tGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGc                                                                                                   <  3:219199/149‑1 (MQ=255)
                                     gCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGcc                                                                                                  <  4:229443/148‑1 (MQ=255)
                                     gCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCt                                                                                                 <  2:248648/149‑1 (MQ=255)
                                             aGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGa                                                                                         <  1:25769/149‑1 (MQ=255)
                                              gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgc                                                                                               >  2:346623/1‑142 (MQ=255)
                                              gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgc                                                                                               <  1:346623/142‑1 (MQ=255)
                                               cTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgcag                                                                                             >  8:236819/1‑143 (MQ=255)
                                               cTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgcag                                                                                             <  7:236819/143‑1 (MQ=255)
                                                   cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                                   <  6:18823/149‑1 (MQ=255)
                                                    aTGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCt                                                                                   >  8:32490/1‑148 (MQ=255)
                                                     tGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTg                                                                                  >  8:143145/1‑148 (MQ=255)
                                                       agaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGaa                                                                                <  8:1592/148‑1 (MQ=255)
                                                        gaTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACa                                                                              >  8:176200/1‑149 (MQ=255)
                                                          tCGCCTAGCAGATCTTGCTAAGTACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGTCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACAt                                                                             <  6:345848/148‑1 (MQ=255)
                                                            gCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGct                                                                          <  7:143145/149‑1 (MQ=255)
                                                              cTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGctct                                                                        >  3:83823/1‑149 (MQ=255)
                                                               tAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTg                                                                       >  7:110152/1‑149 (MQ=255)
                                                                                  cTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATtg                                                    >  8:30232/1‑149 (MQ=255)
                                                                                          ccTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                            <  8:110152/149‑1 (MQ=255)
                                                                                           cTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGCACATGCTCTGTTCGACCGCTGATGGATTGCTGCGTTTg                                           >  4:248968/1‑149 (MQ=255)
                                                                                            tATGGGCGCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                            <  1:83357/147‑1 (MQ=255)
                                                                                            tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGttt                                            >  2:83357/1‑147 (MQ=255)
                                                                                              tGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTggg                                         <  7:30232/148‑1 (MQ=255)
                                                                                                ggCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTa                                       <  1:338832/148‑1 (MQ=255)
                                                                                                        tGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                         >  2:326000/1‑122 (MQ=255)
                                                                                                        tGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                         <  1:326000/122‑1 (MQ=255)
                                                                                                          tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGaa                             <  7:32490/148‑1 (MQ=255)
                                                                                                            gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAAc                            >  1:164100/1‑147 (MQ=255)
                                                                                                                aCCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACa                      <  4:83823/149‑1 (MQ=255)
                                                                                                                 ccGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAg                     <  7:176200/149‑1 (MQ=255)
                                                                                                                 ccGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAg                     >  8:172656/1‑149 (MQ=255)
                                                                                                                  cGAGACGTCGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGc                    >  6:31283/1‑149 (MQ=255)
                                                                                                                  cGAGACGTCGACACCGTCGACGGCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGc                    <  5:31283/149‑1 (MQ=255)
                                                                                                                    aGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCg                   >  1:10787/1‑148 (MQ=255)
                                                                                                                            aCGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGTTATGACTCAGAACGAGACAGCGAAGAAGCTa          <  1:35236/149‑1 (MQ=255)
                                                                                                                             cGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAg         <  5:114720/149‑1 (MQ=255)
                                                                                                                                  tCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTg    <  2:164100/149‑1 (MQ=255)
                                                                                                                                    gACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAAc                            >  7:219013/1‑123 (MQ=255)
                                                                                                                                    gACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAAc                            <  8:219013/123‑1 (MQ=255)
                                                                                                                                    gACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAg         <  6:212374/142‑1 (MQ=255)
                                                                                                                                    gACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAg         >  5:212374/1‑142 (MQ=255)
                                                                                                                                    gACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGcc  >  6:173767/1‑149 (MQ=255)
                                                                                                                                     aCGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCt                                                             <  4:335823/89‑1 (MQ=255)
                                                                                                                                     aCGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCt                                                             >  3:335823/1‑89 (MQ=255)
                                                                                                                                        |                                                                                                                                                
CCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGCC  >  NC_002947/4196166‑4196446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑