Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,205,893 A→G 8.5% M628T (ATG→ACG)  PP_3691 ← DNA helicase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,205,8930AG 8.5% 127.4 / 3.3 47M628T (ATG→ACG) PP_3691DNA helicase‑related protein
Reads supporting (aligned to +/- strand):  ref base A (19/24);  new base G (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

AGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCAGCGAGCCCAAGCCCAGCTCGAAAT  >  NC_002947/4205749‑4206037
                                                                                                                                                |                                                                                                                                                
aGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATc                                                                                                                                                >  5:190879/1‑147 (MQ=255)
aGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATc                                                                                                                                                <  6:190879/147‑1 (MQ=255)
    aCTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGc                                                                                                                                          <  6:3229/149‑1 (MQ=255)
     ctctGCCTCAGGGAAATGAGTATCCTAAACTAACGAATCACGTGGTGTATCCTTCAGTCGCTTAACCACTGGGCTCTGCCGTAGGTGCTCGGCATTTTCGGCCATATCCTTCCACATGAGGTACTTGGCAAACGATAACATCGACAGc                                                                                                                                          <  8:295132/148‑1 (MQ=255)
           cTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGt                                                                                                                                   >  3:92287/1‑149 (MQ=255)
           cTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTAGGCCAGATCCTTCCACATGAGGTACTTGGCAAAAGAGAACATAGAAATCACAACGt                                                                                                                                   >  8:322139/1‑149 (MQ=255)
                 gAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGc                                                                                                                             >  8:9183/1‑149 (MQ=255)
                     ggAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCaa                                                                                                                         >  4:245556/1‑149 (MQ=255)
                         taaccGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACAACTGGGCTCTGCCCCAGGTCCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGAACTTGGCAAACGAGAACATCGACAGCACGACGTCATCGCTCAACTcc                                                                                                                     >  6:197162/4‑149 (MQ=255)
                                 acgaacgaATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTgg                                                                                                             <  3:304507/149‑1 (MQ=255)
                                            aCGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACGTCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGa                                                                                                  >  8:190588/1‑149 (MQ=255)
                                            aCGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACGTCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGa                                                                                                  >  8:195561/1‑149 (MQ=255)
                                             cGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGa                                                                                                  <  3:245556/148‑1 (MQ=255)
                                                    tATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGc                                                                                                                             <  1:233930/114‑1 (MQ=255)
                                                    tATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGa                                                                                                           <  7:250503/132‑1 (MQ=255)
                                                    tATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGa                                                                                                           >  8:250503/1‑132 (MQ=255)
                                                            agGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACttt                                                                                  >  1:17470/1‑149 (MQ=255)
                                                             ggTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTg                                                                                 <  7:122593/149‑1 (MQ=255)
                                                               ttcTGAACCACTGGGCTCTGCCGGAGGTGCTCGGAATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGt                                                                                <  7:322139/146‑1 (MQ=255)
                                                               tGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGtt                                                                               <  4:92287/149‑1 (MQ=255)
                                                               tGCTGAACCACTGGGCTCTGAGGGAGGTGCTCTGAATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGTGATCATCGACAGCTCGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGtt                                                                               <  5:197162/149‑1 (MQ=255)
                                                                    aaCCAGTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTcctg                                                                           <  2:94438/148‑3 (MQ=255)
                                                                    aaCCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACGTCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCa                                                                            <  7:190588/147‑1 (MQ=255)
                                                                    aaCCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACGTCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCa                                                                            <  7:195561/147‑1 (MQ=255)
                                                                    aaCCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCa                                                                            <  7:9183/147‑1 (MQ=255)
                                                                    aaCCACTGGGCACTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCGACATGAGGTACTTTGCAAACGAGTACATCGACAGCACGACGTCTTCGCTCTACTCCCTGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAg                                                                           <  2:89925/148‑1 (MQ=255)
                                                                             gCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGa                                                                 <  3:105969/149‑1 (MQ=255)
                                                                                 tGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAAAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGatat                                                              >  1:271250/1‑148 (MQ=255)
                                                                                      gAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGAc                                                        <  2:17470/149‑1 (MQ=255)
                                                                                        ggTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGAccc                                                      <  8:236501/149‑1 (MQ=255)
                                                                                                   ttttCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTccc                                            >  7:160857/1‑148 (MQ=255)
                                                                                                    tttCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCaa                                                           >  8:321618/1‑132 (MQ=255)
                                                                                                    tttCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCaa                                                           <  7:321618/132‑1 (MQ=255)
                                                                                                         gCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATc                                                                                                                                                <  2:248938/42‑1 (MQ=255)
                                                                                                         gCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATc                                                                                                                                                >  1:248938/1‑42 (MQ=255)
                                                                                                         gCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATc                                                                                                                                                >  1:253663/1‑42 (MQ=255)
                                                                                                         gCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATc                                                                                                                                                <  2:253663/42‑1 (MQ=255)
                                                                                                         gCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAg                                     >  6:11997/1‑149 (MQ=255)
                                                                                                            aGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTc                                  >  4:223803/1‑149 (MQ=255)
                                                                                                                  ttCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTc                            >  5:56937/1‑149 (MQ=255)
                                                                                                                    ccACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCa                          >  2:1727/1‑149 (MQ=255)
                                                                                                                         tGAGGTACATGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGAGGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCAGCGag                     <  6:268546/149‑1 (MQ=255)
                                                                                                                               aCTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTc                                  <  1:307720/130‑1 (MQ=255)
                                                                                                                               aCTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTc                                  >  2:307720/1‑130 (MQ=255)
                                                                                                                                cTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCAGCGagcccaagc              <  2:271250/149‑1 (MQ=255)
                                                                                                                                 ttGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCAGCGagcccaagcc             <  8:160857/149‑1 (MQ=255)
                                                                                                                                            gaACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCAGCGAGCCCAAGCCCAGCTCGAAAt  >  1:176757/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGCGACTCGGCCTCAGGGAAAGGAGTATCCGAAACGAACGAATCACGTGGTGTATCCAGCAGGTGCTGAACCACTGGGCTCTGCCGGAGGTGCTCGGCATTTTCGGCCAGATCCTTCCACATGAGGTACTTGGCAAACGAGAACATCGACAGCACGACGTCTTCGCTCAACTCCCAGCCTGGGATGTCCTTGATAGCATGGCCGACTTTGTTCCAGATGCCTGCGATATCAAGACCCGAATCGTCCCGAGGCAGCTCGTGCTCCAGCGAGCCCAAGCCCAGCTCGAAAT  >  NC_002947/4205749‑4206037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑