Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,244,986 C→A 7.6% G12V (GGC→GTC)  aruH ← arginine‑‑pyruvate transaminase AruH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,244,9860CA 7.6% 193.9 / 4.1 64G12V (GGC→GTC) aruHarginine‑‑pyruvate transaminase AruH
Reads supporting (aligned to +/- strand):  ref base C (32/26);  new base A (2/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CCAGCGCGCAGGCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATTTCTTCCATCTCGGCCT  >  NC_002947/4244839‑4245133
                                                                                                                                                   |                                                                                                                                                   
ccAGCGCGCAGGCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGcc                                                                                                                                                    >  1:50065/1‑149 (MQ=255)
 cAGCGCGCAGGCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCg                                                                                                                                                   >  7:27356/1‑149 (MQ=255)
     gcgcAGGCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGa                                                                                                                                               >  3:175569/1‑149 (MQ=255)
           gCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCATCGCCGGCGACGGCGCTGCggc                                                                                                                                         <  6:31508/149‑3 (MQ=255)
           gCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGtt                                                                                                                                         >  8:39028/1‑149 (MQ=255)
             tGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTgg                                                                                                                                       >  2:84694/1‑149 (MQ=255)
               tCGTTGGCCGCCGCGTCGATAGGCGGGGGAGTGTCGAAGTCGGGGTCAGCCAGCGACAGCAGCATGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGTTGTCCCAGGCCGCAGCGCGGTCGGCGGCGTTGCGTTggg                                                                                                                                      <  6:98603/148‑1 (MQ=255)
                 gATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCACCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCCCGGTAGTGGATGTCCCAGGCCCCACCGCCTTCGCCGGCGATGCGTTGGGt                                                                                                                                     >  8:118709/1‑147 (MQ=255)
                      ccgccTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGt                                                                                                                                     >  8:158006/1‑142 (MQ=255)
                      ccgccTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGt                                                                                                                                     <  7:158006/142‑1 (MQ=255)
                       cgccTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCTGCGATGCGTTGGGTGAGCTTgg                                                                                                                             <  5:246034/149‑1 (MQ=255)
                                    ggCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATgg                                                                                                                 <  8:11120/148‑1 (MQ=255)
                                             gtgtCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCtt                                                                                                       >  5:41728/1‑149 (MQ=255)
                                                tCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTc                                                                                                     >  1:175730/1‑148 (MQ=255)
                                                tCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTc                                                                                                     >  5:156790/1‑148 (MQ=255)
                                                tCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTc                                                                                                     <  5:152911/148‑1 (MQ=255)
                                                tCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTc                                                                                                     >  6:72640/1‑148 (MQ=255)
                                                 cGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCaa                                                                                                   <  1:84694/149‑1 (MQ=255)
                                                      tCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGCGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATgcc                                                                                               >  3:185105/1‑146 (MQ=255)
                                                            tCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCa                                                                                        <  4:329939/149‑1 (MQ=255)
                                                              acccaccGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGt                                                                                      <  4:175569/149‑1 (MQ=255)
                                                                ccaccGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTc                                                                                     >  3:230590/1‑148 (MQ=255)
                                                                 caccGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTcga                                                                                   <  8:267952/149‑1 (MQ=255)
                                                                 caccGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTcga                                                                                   >  3:224757/1‑149 (MQ=255)
                                                                    cGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTcgacga                                                                                <  7:118709/149‑1 (MQ=255)
                                                                            gcagcaTGTCCTTCTCCTCAGCCTGCAGCGACAGGGGGCGGTTGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTc                                                                        <  8:184331/149‑1 (MQ=255)
                                                                                gATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGt                                                                    >  3:218489/1‑149 (MQ=255)
                                                                                      cttgtcctCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAAt                                                              >  7:7229/1‑149 (MQ=255)
                                                                                            ctCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTc                                                                                                     >  4:295869/1‑104 (MQ=255)
                                                                                            ctCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTc                                                                                                     <  3:295869/104‑1 (MQ=255)
                                                                                            ctCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGcccc                                                        <  6:41728/149‑1 (MQ=255)
                                                                                            ctCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGccc                                                         <  4:224757/148‑1 (MQ=255)
                                                                                             tCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCa                                                       >  5:314443/1‑149 (MQ=255)
                                                                                                  cTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTc                                                                        >  4:79800/1‑127 (MQ=255)
                                                                                                  cTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTc                                                                        <  3:79800/127‑1 (MQ=255)
                                                                                                    gCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGgtg                                                >  5:51210/1‑149 (MQ=255)
                                                                                                     cAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGgt                                                 <  8:27356/147‑1 (MQ=255)
                                                                                                           cAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAAt                                         >  5:156343/1‑149 (MQ=255)
                                                                                                            aGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTAc                                             >  8:104226/1‑144 (MQ=255)
                                                                                                            aGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTAc                                             <  7:104226/144‑1 (MQ=255)
                                                                                                            aGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATc                                        <  6:156790/149‑1 (MQ=255)
                                                                                                                 gcgGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGa                                   <  8:7229/149‑1 (MQ=255)
                                                                                                                 gcgGTAGTGGATGTCAGAGGCCGCTGGGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTTGCGCATGGCAATCTCCTTTCAATGGCTATTTGAGTCGACGAGTATCTTCGGGTTGTTATTTCCCCATACGTTGTTCAAATCGGCGa                                   <  4:185105/149‑1 (MQ=255)
                                                                                                                   ggTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGc                                 <  6:156343/149‑1 (MQ=255)
                                                                                                                    gTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGc                                 >  6:133891/1‑148 (MQ=255)
                                                                                                                              tCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGtt                      >  8:285429/1‑149 (MQ=255)
                                                                                                                               cccAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGc                                     >  8:221300/1‑133 (MQ=255)
                                                                                                                               cccAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGc                                     <  7:221300/133‑1 (MQ=255)
                                                                                                                               cccAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGtt                      >  1:14706/1‑148 (MQ=255)
                                                                                                                                ccAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGa                    >  5:305832/1‑149 (MQ=255)
                                                                                                                                ccAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGa                    <  7:39028/149‑1 (MQ=255)
                                                                                                                                ccAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGa                    <  2:175730/149‑1 (MQ=255)
                                                                                                                                    aacGCATCGCCGGCGACGGCGCTGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGa                                   <  2:67440/128‑1 (MQ=255)
                                                                                                                                    aacGCATCGCCGGCGACGGCGCTGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGa                                   >  1:67440/3‑130 (MQ=255)
                                                                                                                                     ccGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATttct               >  3:141232/1‑149 (MQ=255)
                                                                                                                                      cGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATttctt              >  3:46318/1‑149 (MQ=255)
                                                                                                                                       gCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATttcttc             >  6:333400/1‑149 (MQ=255)
                                                                                                                                        cAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATTTCTTcc            <  2:50065/149‑1 (MQ=255)
                                                                                                                                         atcgcCGGCGACGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATTTCTTcc            <  5:40677/146‑1 (MQ=255)
                                                                                                                                         atcgcCGGCGAAGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTAGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGACCAACGAGCAGTTGATTTCTTcc            >  6:40677/3‑148 (MQ=255)
                                                                                                                                          gcgcCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATttct               <  4:238148/144‑1 (MQ=255)
                                                                                                                                          gcgcCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATttct               >  3:238148/1‑144 (MQ=255)
                                                                                                                                            gcCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGGATCTTCGGGTTGGAAGTGCCCCATACGGTGTACAAATCGGCGAGCACCGCGCCGTTGAGTTCTTCCAgct        >  4:43476/1‑149 (MQ=255)
                                                                                                                                             ccGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATTTCTTCCAtctc       <  5:333400/149‑1 (MQ=255)
                                                                                                                                                  gCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATTTCTTCCATCTCGGCCt  >  3:218685/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAGCGCGCAGGCTGTCGATGGCCGCCTCGACGATAGGCGCGGGAGTGTCGAAGTCCGGGTCACCCACCGACAGCAGCAGGATGTCCTTGTCCTCAGCCTGCAGCGCCAGGGCGCGGTAGTGGATGTCCCAGGCCGCAGCGCCGTCGCCGGCGATGCGTTGGGTGAGCTTGGCGTAGCGCATGGCAATCTCCTTTCAATGGCAATTTCAGTCGACGAGTATCTTCGGGTTGGAATTGCCCCATACGGTGTACAAATCGGCGAGCAACGCGCCGTTGATTTCTTCCATCTCGGCCT  >  NC_002947/4244839‑4245133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑