Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,264,918 A→T 11.2% D44V (GAC→GTC)  vanB → vanillate O‑demethylase oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,264,9180AT11.2% 135.1 / 3.0 51D44V (GAC→GTC) vanBvanillate O‑demethylase oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/20);  new base T (2/4);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCGTATCAGTGC  >  NC_002947/4264772‑4265065
                                                                                                                                                  |                                                                                                                                                   
ctgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGTCg                                                                                                                                                   >  8:149122/1‑149 (MQ=255)
 tgAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACgg                                                                                                                                                  >  1:202992/1‑149 (MQ=255)
         aGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGTCGGGCAGGTgc                                                                                                                                          <  1:188350/149‑1 (MQ=255)
          gCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGTCGGGCAGGTgca                                                                                                                                         <  7:17997/149‑2 (MQ=255)
           ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGTCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgcgc                                                                                                                                        >  7:270307/1‑149 (MQ=255)
           ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGTCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgcgc                                                                                                                                        <  8:270307/149‑1 (MQ=255)
           ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGTCGGGCAGGTgcac                                                                                                                                        <  6:165861/149‑3 (MQ=255)
           ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGTCGGGCAGGTgcac                                                                                                                                        <  7:149122/149‑3 (MQ=255)
           ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGAATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATACACTTGCACCAGACCGACCGGCTGGTgcgc                                                                                                                                        >  1:342304/1‑149 (MQ=255)
            cGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgcgc                                                                                                                                        >  3:188699/1‑148 (MQ=255)
                      gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCt                                                                                                                             <  3:109496/149‑1 (MQ=255)
                      gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCt                                                                                                                             <  2:77597/149‑1 (MQ=255)
                        tGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtgt                                                                                                                           <  3:103014/149‑1 (MQ=255)
                            gTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCa                                                                                                                                      >  6:44964/1‑134 (MQ=255)
                            gTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGTCGGGCAGGTgc                                                                                                                                          >  6:116699/1‑130 (MQ=255)
                               gTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAacca                                                                                                                    >  6:131960/1‑149 (MQ=255)
                                tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAaccac                                                                                                                   <  2:202992/149‑1 (MQ=255)
                                    aTCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCAcccc                                                                                                                >  1:227161/1‑148 (MQ=255)
                                       ccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCgaa                                                                                                             <  4:188699/148‑1 (MQ=255)
                                       ccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCgaa                                                                                                             <  2:101588/148‑1 (MQ=255)
                                       ccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCg                                                                                                               >  1:101588/1‑146 (MQ=255)
                                                    gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTa                                                                                               >  8:23369/1‑149 (MQ=255)
                                                    gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTa                                                                                               >  2:336150/1‑149 (MQ=255)
                                                    gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTa                                                                                               >  4:182404/1‑149 (MQ=255)
                                                    gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACCGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTa                                                                                               >  5:131212/1‑149 (MQ=255)
                                                                     cTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTc                                                                               >  6:343154/1‑148 (MQ=255)
                                                                          aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAc                                                                         <  7:23369/149‑1 (MQ=255)
                                                                          aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAc                                                                         <  3:182404/149‑1 (MQ=255)
                                                                                     gCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgcg                                                              <  5:131960/149‑1 (MQ=255)
                                                                                        gATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg                                                           <  1:336150/149‑1 (MQ=255)
                                                                                             cAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt                                                      >  8:89328/1‑149 (MQ=255)
                                                                                                    ctgcCGGCGTTCAGCGCGGGCGCCGATATCGACGTGCACCTGCCCGACGGGCTGGTGCGTGAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGTGCGGTTCTCGTa                                                <  2:342304/148‑1 (MQ=255)
                                                                                                    ctgcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAg                                               <  7:89328/149‑1 (MQ=255)
                                                                                                         ggCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTg                                           >  5:35357/1‑148 (MQ=255)
                                                                                                             ttCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGc                                              <  8:20725/141‑1 (MQ=255)
                                                                                                             ttCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGc                                              >  7:20725/1‑141 (MQ=255)
                                                                                                                aGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgg                                                             >  1:232000/1‑123 (MQ=255)
                                                                                                                aGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgg                                                             <  2:232000/123‑1 (MQ=255)
                                                                                                                     cGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTg                               >  6:290677/1‑148 (MQ=255)
                                                                                                                            cATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc                                        >  6:289099/1‑132 (MQ=255)
                                                                                                                            cATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc                                        <  5:289099/132‑1 (MQ=255)
                                                                                                                               atCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGc                    >  8:52573/1‑149 (MQ=255)
                                                                                                                                  gACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCg                 <  6:35357/149‑1 (MQ=255)
                                                                                                                                     gTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCt              <  7:52573/149‑1 (MQ=255)
                                                                                                                                     gTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCt              >  3:249526/1‑149 (MQ=255)
                                                                                                                                        cACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAATGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCg           >  3:181949/1‑149 (MQ=255)
                                                                                                                                           cTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCGTa         >  6:18584/1‑148 (MQ=255)
                                                                                                                                           cTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCGTAt        >  1:187955/1‑149 (MQ=255)
                                                                                                                                           cTGCCAGACGGGCTGGTGCGCCAGTATTAGCTGTGCAACCCCCCCGAAGAACGCCATCGCTAACTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCAGCGTAt        >  6:93961/1‑149 (MQ=255)
                                                                                                                                              cccGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCGTATCa      <  5:343154/148‑1 (MQ=255)
                                                                                                                                                 gACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCGTATCAGTgc  <  6:131212/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTGAGGAACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCGGCTGCGTATCAGTGC  >  NC_002947/4264772‑4265065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑