Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,273,044	T→C	10.5%	intergenic (+20/+41)	PP_3743 → / ← glcC	hypothetical protein/transcriptional regulator GlcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,273,044	0	T	C	10.5%	175.5 / 9.1	66	intergenic (+20/+41)	PP_3743/glcC	hypothetical protein/transcriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base T (34/25);  new base C (4/3);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCCGCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGC  >  NC_002947/4272898‑4273189                                                                                                                                                   |                                                                                                                                                    cATCCGCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTc                                                                                                                                                   <  1:238786/149‑1 (MQ=255)  aTCCGCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTcg                                                                                                                                                  <  3:6428/149‑1 (MQ=255)   tCCGCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTccc                                                                                                                                                 >  7:277598/1‑147 (MQ=255)      gcggcgCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTcgcgtc                                                                                                                                              >  7:314394/1‑147 (MQ=255)      gcggcgCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCgcggc                                                                                                                                              <  7:278762/149‑1 (MQ=255)               aTGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCAGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcg                                                                                                                                     >  7:182579/1‑149 (MQ=255)                  tgGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                    >  1:313063/1‑147 (MQ=255)                   ggAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                    >  7:68575/1‑146 (MQ=255)                      gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                              <  8:314394/149‑1 (MQ=38)                      gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                              <  4:159314/149‑1 (MQ=38)                         ggCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGCCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGcc                                                                                                                           >  1:80982/1‑149 (MQ=255)                            gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCGTTCGCGGCGGTGCGCCGCGAGCGGGcc                                                                                                                           >  6:199672/1‑146 (MQ=255)                              ttGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCgaga                                                                                                                                >  6:309551/1‑139 (MQ=255)                              ttGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCgaga                                                                                                                                <  5:309551/139‑1 (MQ=255)                                 aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                    <  2:249961/148‑1 (MQ=255)                                  aCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAgg                                                                                                                  <  8:68575/149‑1 (MQ=255)                                   cAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGTGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGtt                                                                                                                                          <  3:32943/124‑1 (MQ=255)                                   cAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGAACCTGTGGCTGAACATTGAGTAAACCTGTACAGGCACTTTCGcggcgggt                                                                                                                                          >  4:32943/1‑122 (MQ=255)                                        ccGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCtt                                                                                                            >  6:156480/1‑149 (MQ=255)                                           cAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAc                                                                                                         <  1:339422/149‑1 (MQ=255)                                               gACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAccccc                                                                                                     >  8:42916/1‑149 (MQ=255)                                               gACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAccccc                                                                                                     <  5:156480/149‑1 (MQ=255)                                                       tGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGccatc                                                                                              >  1:345570/1‑148 (MQ=255)                                                          ggtGCTGGCCAACGACGAAGAGCTGGCCCGGGGCATCCTGTCGCAAGATGACCCTGTGGATGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATa                                                                                          <  8:277598/147‑1 (MQ=255)                                                              cAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                      >  3:82432/1‑149 (MQ=255)                                                                ggCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                      <  7:184137/147‑1 (MQ=255)                                                                ggCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                      >  8:184137/1‑147 (MQ=255)                                                                   cAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCa                                                                                                          >  6:348374/1‑124 (MQ=255)                                                                   cAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTc                                                                               >  5:47759/1‑149 (MQ=255)                                                                    aaCGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCg                                                                              <  4:82432/149‑1 (MQ=255)                                                                       gacgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGccc                                                                           <  8:237706/149‑1 (MQ=255)                                                                        acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCt                                                                          <  4:188344/149‑1 (MQ=255)                                                                        acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCt                                                                          <  6:106448/149‑1 (MQ=255)                                                                          gaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGa                                                                        >  7:208061/1‑149 (MQ=255)                                                                             gagCTGGACAGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAACCCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCa                                                                     <  6:271044/149‑1 (MQ=255)                                                                                   gCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGCCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGgcgc                                                                <  5:199672/148‑1 (MQ=255)                                                                                   gCCCGGGCCATCCTGTCGCAAGATGACCCTGGGGCTGACCATTTATTAAACCTGTTCCGGCCCTTTCGCGGGGGTTCTACGCTAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGgcgc                                                                <  2:313063/148‑1 (MQ=255)                                                                                     ccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCgctgc                                                             >  8:205043/1‑149 (MQ=255)                                                                                      cGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCgctgct                                                            >  5:353588/1‑149 (MQ=255)                                                                                            aTCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCt                                                      >  2:102677/1‑149 (MQ=255)                                                                                                    gCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAtc                                              >  2:247153/1‑149 (MQ=255)                                                                                                      aaGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAtctc                                            >  5:338403/1‑149 (MQ=255)                                                                                                        gATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTcgc                                                                                                                                                 <  1:102727/48‑1 (MQ=255)                                                                                                        gATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTcgc                                                                                                                                                 >  2:102727/1‑48 (MQ=255)                                                                                                                    gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGcc                                                                            <  6:288247/103‑1 (MQ=255)                                                                                                                    gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGcc                                                                            >  5:288247/1‑103 (MQ=255)                                                                                                                    gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                              >  7:318450/1‑149 (MQ=255)                                                                                                                          cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGc                        >  3:50214/1‑149 (MQ=255)                                                                                                                           aTTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTAGACGCGAGAGGGCCGATACAAGGCAGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCTCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTAGCTTAGGcc                       >  2:262756/1‑149 (MQ=255)                                                                                                                            ttGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcg                               <  1:97696/140‑1 (MQ=255)                                                                                                                            ttGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcg                               >  2:97696/1‑140 (MQ=255)                                                                                                                              gAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGt                     <  1:102677/148‑1 (MQ=255)                                                                                                                               aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGCCCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                    >  5:80120/1‑148 (MQ=255)                                                                                                                               aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAACCTGTCGCGTATGCCGTTg                   >  5:331237/1‑149 (MQ=255)                                                                                                                               aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                    <  6:338403/148‑1 (MQ=255)                                                                                                                                gTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGa                  <  8:182579/149‑1 (MQ=255)                                                                                                                                 tAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGa                  >  2:47527/1‑148 (MQ=255)                                                                                                                                     cttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGc             >  3:43595/3‑149 (MQ=37)                                                                                                                                     ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGctc                                                           <  1:237593/103‑1 (MQ=255)                                                                                                                                     ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGctc                                                           >  2:237593/1‑103 (MQ=255)                                                                                                                                      ttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                              >  5:315534/2‑131 (MQ=25)                                                                                                                                      ttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                              <  6:315534/130‑1 (MQ=25)                                                                                                                                      cTGTACCGGCCCTTTCGCGGCGGTTCGCCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCt            >  2:236160/1‑149 (MQ=255)                                                                                                                                         tatcGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAGGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAc         >  3:22650/4‑149 (MQ=37)                                                                                                                                                ccTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGcgccgc  >  8:222532/1‑149 (MQ=255)                                                                                                                                                ccTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGAATG‑‑GTCGCGCTGACGAGGCGCTGCTCTTCCTGCACGATCTCCCGGAAACTGTCGCGTATGCCGTTGTGATGCTGACGcgccgc  >  8:222535/1‑149 (MQ=255)                                                                                                                                                  tttCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGTCGGCGCCATGACCAGGCGCTGCTCTTCCTGATCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGcgccgc  >  1:280120/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CATCCGCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGC  >  NC_002947/4272898‑4273189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑