Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,273,050	G→T	10.0%	intergenic (+26/+35)	PP_3743 → / ← glcC	hypothetical protein/transcriptional regulator GlcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,273,050	0	G	T	10.0%	172.8 / 8.5	60	intergenic (+26/+35)	PP_3743/glcC	hypothetical protein/transcriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base G (32/22);  new base T (3/3);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCG  >  NC_002947/4272903‑4273192                                                                                                                                                    |                                                                                                                                                 gcggcgCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCgcggc                                                                                                                                                 <  7:278762/149‑1 (MQ=255)          aTGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCAGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcg                                                                                                                                        >  7:182579/1‑149 (MQ=255)             tgGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                       >  1:313063/1‑147 (MQ=255)              ggAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                       >  7:68575/1‑146 (MQ=255)                 gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                 <  4:159314/149‑1 (MQ=38)                 gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                 <  8:314394/149‑1 (MQ=38)                    ggCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGCCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGcc                                                                                                                              >  1:80982/1‑149 (MQ=255)                       gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCGTTCGCGGCGGTGCGCCGCGAGCGGGcc                                                                                                                              >  6:199672/1‑146 (MQ=255)                         ttGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCgaga                                                                                                                                   >  6:309551/1‑139 (MQ=255)                         ttGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCgaga                                                                                                                                   <  5:309551/139‑1 (MQ=255)                            aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                       <  2:249961/148‑1 (MQ=255)                             aCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAgg                                                                                                                     <  8:68575/149‑1 (MQ=255)                              cAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGTGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGtt                                                                                                                                             <  3:32943/124‑1 (MQ=255)                              cAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGAACCTGTGGCTGAACATTGAGTAAACCTGTACAGGCACTTTCGcggcgggt                                                                                                                                             >  4:32943/1‑122 (MQ=255)                                   ccGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCtt                                                                                                               >  6:156480/1‑149 (MQ=255)                                      cAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAc                                                                                                            <  1:339422/149‑1 (MQ=255)                                          gACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAccccc                                                                                                        <  5:156480/149‑1 (MQ=255)                                          gACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAccccc                                                                                                        >  8:42916/1‑149 (MQ=255)                                                  tGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGccatc                                                                                                 >  1:345570/1‑148 (MQ=255)                                                     ggtGCTGGCCAACGACGAAGAGCTGGCCCGGGGCATCCTGTCGCAAGATGACCCTGTGGATGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATa                                                                                             <  8:277598/147‑1 (MQ=255)                                                         cAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                         >  3:82432/1‑149 (MQ=255)                                                           ggCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                         >  8:184137/1‑147 (MQ=255)                                                           ggCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                         <  7:184137/147‑1 (MQ=255)                                                              cAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCa                                                                                                             >  6:348374/1‑124 (MQ=255)                                                              cAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTc                                                                                  >  5:47759/1‑149 (MQ=255)                                                               aaCGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCg                                                                                 <  4:82432/149‑1 (MQ=255)                                                                  gacgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGccc                                                                              <  8:237706/149‑1 (MQ=255)                                                                   acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCt                                                                             <  4:188344/149‑1 (MQ=255)                                                                   acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCt                                                                             <  6:106448/149‑1 (MQ=255)                                                                     gaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGa                                                                           >  7:208061/1‑149 (MQ=255)                                                                        gagCTGGACAGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAACCCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCa                                                                        <  6:271044/149‑1 (MQ=255)                                                                              gCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGCCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGgcgc                                                                   <  5:199672/148‑1 (MQ=255)                                                                              gCCCGGGCCATCCTGTCGCAAGATGACCCTGGGGCTGACCATTTATTAAACCTGTTCCGGCCCTTTCGCGGGGGTTCTACGCTAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGgcgc                                                                   <  2:313063/148‑1 (MQ=255)                                                                                ccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCgctgc                                                                >  8:205043/1‑149 (MQ=255)                                                                                 cGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCgctgct                                                               >  5:353588/1‑149 (MQ=255)                                                                                       aTCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCt                                                         >  2:102677/1‑149 (MQ=255)                                                                                               gCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAtc                                                 >  2:247153/1‑149 (MQ=255)                                                                                                 aaGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAtctc                                               >  5:338403/1‑149 (MQ=255)                                                                                                               gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGcc                                                                               >  5:288247/1‑103 (MQ=255)                                                                                                               gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGcc                                                                               <  6:288247/103‑1 (MQ=255)                                                                                                               gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                                 >  7:318450/1‑149 (MQ=255)                                                                                                                     cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGc                           >  3:50214/1‑149 (MQ=255)                                                                                                                      aTTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTAGACGCGAGAGGGCCGATACAAGGCAGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCTCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTAGCTTAGGcc                          >  2:262756/1‑149 (MQ=255)                                                                                                                       ttGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcg                                  <  1:97696/140‑1 (MQ=255)                                                                                                                       ttGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcg                                  >  2:97696/1‑140 (MQ=255)                                                                                                                         gAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGt                        <  1:102677/148‑1 (MQ=255)                                                                                                                          aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGCCCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                       >  5:80120/1‑148 (MQ=255)                                                                                                                          aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAACCTGTCGCGTATGCCGTTg                      >  5:331237/1‑149 (MQ=255)                                                                                                                          aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                       <  6:338403/148‑1 (MQ=255)                                                                                                                           gTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGa                     <  8:182579/149‑1 (MQ=255)                                                                                                                            tAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGa                     >  2:47527/1‑148 (MQ=255)                                                                                                                                cttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGc                >  3:43595/3‑149 (MQ=37)                                                                                                                                ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGctc                                                              <  1:237593/103‑1 (MQ=255)                                                                                                                                ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGctc                                                              >  2:237593/1‑103 (MQ=255)                                                                                                                                 ttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                                 <  6:315534/130‑1 (MQ=25)                                                                                                                                 ttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                                 >  5:315534/2‑131 (MQ=25)                                                                                                                                 cTGTACCGGCCCTTTCGCGGCGGTTCGCCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCt               >  2:236160/1‑149 (MQ=255)                                                                                                                                    tatcGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAGGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAc            >  3:22650/4‑149 (MQ=37)                                                                                                                                           ccTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGcgccgc     >  8:222532/1‑149 (MQ=255)                                                                                                                                           ccTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGAATG‑‑GTCGCGCTGACGAGGCGCTGCTCTTCCTGCACGATCTCCCGGAAACTGTCGCGTATGCCGTTGTGATGCTGACGcgccgc     >  8:222535/1‑149 (MQ=255)                                                                                                                                             tttCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGTCGGCGCCATGACCAGGCGCTGCTCTTCCTGATCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGcgccgc     >  1:280120/1‑149 (MQ=255)                                                                                                                                               tCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGccgcccg  <  6:47759/148‑1 (MQ=255)                                                                                                                                                 gcggcgGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                       <  8:182287/125‑1 (MQ=255)                                                                                                                                                 gcggcgGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                       >  7:182287/1‑125 (MQ=255)                                                                                                                                                   ggcggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                       <  3:329379/123‑1 (MQ=255)                                                                                                                                                   ggcggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                       >  4:329379/1‑123 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GCGGCGCCTATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCG  >  NC_002947/4272903‑4273192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑