Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,273,055 T→C 9.4% intergenic (+31/+30) PP_3743 → / ← glcC hypothetical protein/transcriptional regulator GlcC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,273,0550TC 9.4% 172.9 / 8.1 64intergenic (+31/+30)PP_3743/glcChypothetical protein/transcriptional regulator GlcC
Reads supporting (aligned to +/- strand):  ref base T (33/24);  new base C (3/3);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01

ATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCT  >  NC_002947/4272912‑4273200
                                                                                                                                               |                                                                                                                                                   
aTGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCAGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcg                                                                                                                                                >  7:182579/1‑149 (MQ=255)
   tgGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                               >  1:313063/1‑147 (MQ=255)
    ggAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGAcgc                                                                                                                                               >  7:68575/1‑146 (MQ=255)
       gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                         <  4:159314/149‑1 (MQ=38)
       gTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAgg                                                                                                                                         <  8:314394/149‑1 (MQ=38)
          ggCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGCCCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGcc                                                                                                                                      >  1:80982/1‑149 (MQ=255)
             gcTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCGTTCGCGGCGGTGCGCCGCGAGCGGGcc                                                                                                                                      >  6:199672/1‑146 (MQ=255)
               ttGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCgaga                                                                                                                                           >  6:309551/1‑139 (MQ=255)
               ttGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCgaga                                                                                                                                           <  5:309551/139‑1 (MQ=255)
                  aaCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACaa                                                                                                                               <  2:249961/148‑1 (MQ=255)
                   aCAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAgg                                                                                                                             <  8:68575/149‑1 (MQ=255)
                    cAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGTGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGtt                                                                                                                                                     <  3:32943/124‑1 (MQ=255)
                         ccGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCtt                                                                                                                       >  6:156480/1‑149 (MQ=255)
                            cAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAc                                                                                                                    <  1:339422/149‑1 (MQ=255)
                                gACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAccccc                                                                                                                >  8:42916/1‑149 (MQ=255)
                                gACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCAccccc                                                                                                                <  5:156480/149‑1 (MQ=255)
                                        tGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGccatc                                                                                                         >  1:345570/1‑148 (MQ=255)
                                           ggtGCTGGCCAACGACGAAGAGCTGGCCCGGGGCATCCTGTCGCAAGATGACCCTGTGGATGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATa                                                                                                     <  8:277598/147‑1 (MQ=255)
                                               cAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                                 >  3:82432/1‑149 (MQ=255)
                                                 ggCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                                 >  8:184137/1‑147 (MQ=255)
                                                 ggCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCa                                                                                                 <  7:184137/147‑1 (MQ=255)
                                                    cAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCa                                                                                                                     >  6:348374/1‑124 (MQ=255)
                                                    cAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTc                                                                                          >  5:47759/1‑149 (MQ=255)
                                                     aaCGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCg                                                                                         <  4:82432/149‑1 (MQ=255)
                                                        gacgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGccc                                                                                      <  8:237706/149‑1 (MQ=255)
                                                         acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCt                                                                                     <  6:106448/149‑1 (MQ=255)
                                                         acgaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCt                                                                                     <  4:188344/149‑1 (MQ=255)
                                                           gaAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGa                                                                                   >  7:208061/1‑149 (MQ=255)
                                                              gagCTGGACAGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAACCCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCa                                                                                <  6:271044/149‑1 (MQ=255)
                                                                    gCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGCCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGgcgc                                                                           <  5:199672/148‑1 (MQ=255)
                                                                    gCCCGGGCCATCCTGTCGCAAGATGACCCTGGGGCTGACCATTTATTAAACCTGTTCCGGCCCTTTCGCGGGGGTTCTACGCTAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGgcgc                                                                           <  2:313063/148‑1 (MQ=255)
                                                                      ccGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCgctgc                                                                        >  8:205043/1‑149 (MQ=255)
                                                                       cGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCgctgct                                                                       >  5:353588/1‑149 (MQ=255)
                                                                             aTCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCt                                                                 >  2:102677/1‑149 (MQ=255)
                                                                                     gCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAtc                                                         >  2:247153/1‑149 (MQ=255)
                                                                                       aaGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGAtctc                                                       >  5:338403/1‑149 (MQ=255)
                                                                                                     gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGcc                                                                                       <  6:288247/103‑1 (MQ=255)
                                                                                                     gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGcc                                                                                       >  5:288247/1‑103 (MQ=255)
                                                                                                     gCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                                         >  7:318450/1‑149 (MQ=255)
                                                                                                           cATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGc                                   >  3:50214/1‑149 (MQ=255)
                                                                                                            aTTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTAGACGCGAGAGGGCCGATACAAGGCAGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCTCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTAGCTTAGGcc                                  >  2:262756/1‑149 (MQ=255)
                                                                                                             ttGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcg                                          >  2:97696/1‑140 (MQ=255)
                                                                                                             ttGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcg                                          <  1:97696/140‑1 (MQ=255)
                                                                                                               gAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGt                                <  1:102677/148‑1 (MQ=255)
                                                                                                                aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGCCCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                               >  5:80120/1‑148 (MQ=255)
                                                                                                                aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAACCTGTCGCGTATGCCGTTg                              >  5:331237/1‑149 (MQ=255)
                                                                                                                aGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                               <  6:338403/148‑1 (MQ=255)
                                                                                                                 gTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGa                             <  8:182579/149‑1 (MQ=255)
                                                                                                                  tAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGa                             >  2:47527/1‑148 (MQ=255)
                                                                                                                      cttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGc                        >  3:43595/3‑149 (MQ=37)
                                                                                                                      ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGctc                                                                      <  1:237593/103‑1 (MQ=255)
                                                                                                                      ccTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGctc                                                                      >  2:237593/1‑103 (MQ=255)
                                                                                                                       ttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                                         <  6:315534/130‑1 (MQ=25)
                                                                                                                       ttGTATCGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTcgc                                         >  5:315534/2‑131 (MQ=25)
                                                                                                                       cTGTACCGGCCCTTTCGCGGCGGTTCGCCGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCt                       >  2:236160/1‑149 (MQ=255)
                                                                                                                          tatcGGCCCTCTCGCGTCGAACCGCCGCGAAAGGGCCGGTACAGGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCAc                    >  3:22650/4‑149 (MQ=37)
                                                                                                                                 ccTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGcgccgc             >  8:222532/1‑149 (MQ=255)
                                                                                                                                 ccTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGAATG‑‑GTCGCGCTGACGAGGCGCTGCTCTTCCTGCACGATCTCCCGGAAACTGTCGCGTATGCCGTTGTGATGCTGACGcgccgc             >  8:222535/1‑149 (MQ=255)
                                                                                                                                   tttCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATGTCGGCGCCATGACCAGGCGCTGCTCTTCCTGATCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGcgccgc             >  1:280120/1‑149 (MQ=255)
                                                                                                                                     tCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGccgcccg          <  6:47759/148‑1 (MQ=255)
                                                                                                                                       gcggcgGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                               >  7:182287/1‑125 (MQ=255)
                                                                                                                                       gcggcgGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                               <  8:182287/125‑1 (MQ=255)
                                                                                                                                         ggcggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                               >  4:329379/1‑123 (MQ=255)
                                                                                                                                         ggcggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGtt                               <  3:329379/123‑1 (MQ=255)
                                                                                                                                            ggTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCt  <  1:247153/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ATGTGGAGTCGGCGCTTGAACAGGCCCGCAAGGACCATCCTGGGCTGCAGGCCAACGACGAAGAGCTGGCCCGGGCCATCCTGTCGCAAGATGACCCTGTGGCTGACCATTGAGTAAACCTGTACCGGCCCTTTCGCGGCGGTTCGACGCGAGAGGGCCGATACAAGGCCGCTTCACCCCCAGCCATCCATACGCATG‑‑GTCGCCCTGACCAGGCGCTGCTCTTCCTGCTCGATCTCCCGCAAACTGTCGCGTATGCCGTTGATATGCTCACGCGCCGCCCGCTGCGCCT  >  NC_002947/4272912‑4273200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑