Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,476,301 A→G 7.1% intergenic (‑50/‑126) PP_3966 ← / → PP_3967 transposase/MutT/nudix family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,476,3010AG 7.1% 152.4 / 2.4 57intergenic (‑50/‑126)PP_3966/PP_3967transposase/MutT/nudix family protein
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base G (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

CGACACTGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGTGAACTTCCAGATGCAGGTATGGCCTTCCGTT  >  NC_002947/4476164‑4476447
                                                                                                                                         |                                                                                                                                                  
cGACACTGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCt                                                                                                                                          <  7:160006/148‑1 (MQ=32)
   cacTGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAAt                                                                                                                                      <  8:18208/149‑1 (MQ=32)
   cacTGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAAt                                                                                                                                      <  6:220377/149‑1 (MQ=32)
   cacTGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTatc                                                                                                                                      <  2:235279/149‑3 (MQ=11)
      tGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGGTAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCt                                                                                                                                   >  3:219449/1‑149 (MQ=17)
      tGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCa                                                                                                                                                 >  8:65973/1‑135 (MQ=32)
      tGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCa                                                                                                                                                 <  7:65973/135‑1 (MQ=32)
      tGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCt                                                                                                                                   >  8:191217/1‑149 (MQ=32)
         acacGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGa                                                                                                                                <  7:188769/149‑1 (MQ=32)
           acGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGt                                                                                                                              >  1:42118/1‑149 (MQ=32)
             ggTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTc                                                                                                                            <  5:267844/149‑1 (MQ=32)
                  cgcCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAg                                                                                                                       >  8:102214/1‑149 (MQ=32)
                   gcCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAgg                                                                                                                      >  5:24127/1‑149 (MQ=32)
                    ccAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCt                                                                                                                                   <  8:47605/135‑1 (MQ=32)
                    ccAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCt                                                                                                                                   >  7:47605/1‑135 (MQ=32)
                      aGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGGTAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGa                                                                                                                   <  4:219449/149‑1 (MQ=17)
                        gTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGt                                                                                                                 <  1:259427/149‑1 (MQ=32)
                                 aTTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGACGGAc                                                                                                        >  8:279993/1‑149 (MQ=17)
                                    ccTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCg                                                                                                           >  8:6959/1‑143 (MQ=32)
                                    ccTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCg                                                                                                           <  7:6959/143‑1 (MQ=32)
                                    ccTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCt                                                                                                     >  6:312183/1‑149 (MQ=32)
                                     cTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCtt                                                                                                    <  2:72445/149‑1 (MQ=32)
                                        aaCGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTAc                                                                                                  <  7:102214/148‑1 (MQ=32)
                                             ccTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGa                                                                                                                                <  6:150073/113‑1 (MQ=32)
                                             ccTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGa                                                                                                                                >  5:150073/1‑113 (MQ=32)
                                                     ttGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGc                                                                                             <  1:311827/140‑1 (MQ=34)
                                                     ttGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGc                                                                                             >  2:311827/1‑140 (MQ=34)
                                                           ggTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCt                                                                              <  5:312183/149‑1 (MQ=255)
                                                            gTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCt                                                                              >  6:114741/1‑148 (MQ=255)
                                                             tttCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTga                                                                            <  2:49505/149‑1 (MQ=255)
                                                               tCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTgag                                                                           <  5:166433/148‑1 (MQ=255)
                                                                 ggATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAgg                                                                        >  4:58975/1‑149 (MQ=255)
                                                                   aTAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCAt                                                                                 >  3:246934/1‑138 (MQ=255)
                                                                   aTAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCAt                                                                                 <  4:246934/138‑1 (MQ=255)
                                                                       gagCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTg                                                                  >  1:288769/1‑149 (MQ=255)
                                                                       gagCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTg                                                                  >  6:124056/1‑149 (MQ=255)
                                                                          cTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACCCCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCCTGCTGAGAGGTTGGTGCac                                                               >  8:227810/1‑148 (MQ=255)
                                                                          cTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCaa                                                               <  5:114741/149‑1 (MQ=255)
                                                                           ttCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTg                                                                     <  6:295401/142‑1 (MQ=255)
                                                                           ttCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTg                                                                     >  5:295401/1‑142 (MQ=255)
                                                                              gTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGAc                                                           <  2:288769/149‑1 (MQ=255)
                                                                                    gcATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACa                                                     <  6:24127/149‑1 (MQ=255)
                                                                                       tGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGCCCCGACAGGc                                                  >  4:91566/1‑149 (MQ=255)
                                                                                       tGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGc                                                  <  7:191217/149‑1 (MQ=255)
                                                                                            aTCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTc                                             <  6:24145/149‑1 (MQ=255)
                                                                                                tGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCAcc                                          >  4:126844/1‑148 (MQ=255)
                                                                                                cagcattAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCAcc                                          >  6:16090/7‑148 (MQ=21)
                                                                                                  gcattAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTAATGGTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATGATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAg                                       >  5:106905/5‑149 (MQ=21)
                                                                                                  gCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCg                                         <  5:124056/147‑1 (MQ=255)
                                                                                                        aGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGt                                 <  2:42118/149‑1 (MQ=255)
                                                                                                         gggTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGt                                 >  6:268941/1‑148 (MQ=255)
                                                                                                          ggTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGTg                                >  8:195606/1‑148 (MQ=255)
                                                                                                                           aaaaaTACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATg                                                                                >  4:322355/1‑83 (MQ=255)
                                                                                                                           aaaaaTACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATg                                                                                <  3:322355/83‑1 (MQ=255)
                                                                                                                                taagcgGACGCGATCACCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGTGAACTTCCAGATGCAGGTATGGc          <  4:317882/145‑1 (MQ=255)
                                                                                                                                   gcgGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGTGAACTTCCAGATGCAGGTATGGCCTTc      >  4:68190/1‑149 (MQ=255)
                                                                                                                                      gACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGTGAACTTCCAGATGCAGGTATGGCCTTCCGt   >  2:86652/1‑149 (MQ=255)
                                                                                                                                       acacCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCTCCGAGCGGAGTGAACTTCCAGATGCAGGTATGGCCTTCCGtt  <  7:195606/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
CGACACTGGACACGGTTGCGCCAGGTTGCAGGCATTCCTGAACGACCTGGGCTTTGAACGGTTTCGGATAAGAGCTTCGTTGGCGCATGGAAATCCTGGCGATAAGGGTGATCGCGTCCGCTTAAAAATACGCGGACACCATCGCCCTTAATGCTGGAGTTCGGAAGGTGAGTTCGCCGGACGCTTACGAAGCGGCTGGAATCATGCTGAGAGGTTGGTGCAACGACCCGACAGGCCATTCACCGAGCGGAGTGAACTTCCAGATGCAGGTATGGCCTTCCGTT  >  NC_002947/4476164‑4476447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑