| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,501,297 | A→T | 10.5% | intergenic (‑236/+19) | PP_3989 ← / ← PP_t60 | DNA‑cytosine methyltransferase/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,501,297 | 0 | A | T | 10.5% | 155.1 / 8.6 | 60 | intergenic (‑236/+19) | PP_3989/PP_t60 | DNA‑cytosine methyltransferase/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (29/24); new base T (4/2); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-01 | |||||||||||
GAAAAGCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACAC > NC_002947/4501150‑4501442 | gAAAAGCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGaa > 5:290643/1‑148 (MQ=255) gCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCa < 2:226812/149‑1 (MQ=255) tctAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGATTATTCAgg > 3:233866/1‑149 (MQ=255) tctAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAgg > 1:20004/1‑149 (MQ=255) tAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGc > 7:106799/1‑149 (MQ=255) gggAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttt > 8:248246/1‑149 (MQ=255) gggAAGCGCGTCACGCCAAGCTGAAGCAGCTCACACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttt > 3:309715/1‑149 (MQ=255) gAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGTAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGATTATTCATGGCttttt < 4:233866/149‑3 (MQ=255) gAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGGGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTg > 5:294974/1‑148 (MQ=255) gAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGATTATTCAGGGCttttt < 3:268818/149‑3 (MQ=255) aGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAat < 8:205313/149‑1 (MQ=255) aGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAat < 3:333706/149‑1 (MQ=255) cgcgTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAatat > 2:67723/1‑149 (MQ=255) gTCACGCCAAGCTGAAGCAGCTCACACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGc < 4:309715/149‑1 (MQ=255) aCGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATAtggtgg > 7:153599/1‑148 (MQ=255) aCGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCgg > 7:111303/1‑148 (MQ=255) cGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGaa < 2:185531/149‑1 (MQ=255) gAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTa > 3:118549/1‑145 (MQ=255) gcagcTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGGATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAGGCGTAGAGATTc > 2:133865/1‑149 (MQ=255) gcagcTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTc < 6:290643/149‑1 (MQ=255) gcTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGaa < 3:182705/149‑1 (MQ=255) tCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAAc > 2:21227/1‑148 (MQ=255) gCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAActc < 3:189254/148‑1 (MQ=255) aGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATa < 8:111303/149‑1 (MQ=255) aTGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGtt > 8:172232/1‑149 (MQ=255) tgtCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCATGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGc < 8:239869/148‑1 (MQ=255) cGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACg < 7:248246/148‑1 (MQ=255) gAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGaa > 6:241229/1‑107 (MQ=255) gAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGaa < 5:241229/107‑1 (MQ=255) aaaCGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGCCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGttt > 7:247496/1‑149 (MQ=255) aCGATCAGAGAATTGCGGAAACGACCCGTATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTc < 7:271745/149‑1 (MQ=255) aCGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGTGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTc < 8:153599/149‑1 (MQ=255) gATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCaa < 1:21227/149‑1 (MQ=255) gagaATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATc > 5:279680/1‑76 (MQ=255) gagaATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATc < 6:279680/76‑1 (MQ=255) gagaATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCg > 7:10731/1‑149 (MQ=255) aTTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGc < 7:172232/149‑1 (MQ=255) gCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCt < 2:20004/148‑1 (MQ=255) cGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTa < 1:67723/149‑1 (MQ=255) gAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTaaa < 6:294974/149‑1 (MQ=255) gACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAGGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACt < 1:133865/149‑1 (MQ=255) cccGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCt < 3:249065/138‑1 (MQ=255) cccGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCt > 4:249065/1‑138 (MQ=255) aGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTc > 7:138020/1‑149 (MQ=255) gAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTc > 4:79925/1‑149 (MQ=255) aaaaaaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGATTATGGCGGAAGCGTAGAGATTCGAACTCTAGGATTGTTGCCCATCGACGGTTTTCAAGACCGTTGCGTTAATCCACTCGGCCACGCTTCCATCTCg > 2:112060/1‑149 (MQ=37) aaaaaaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCg > 8:165167/1‑149 (MQ=255) aaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGtttt < 4:118549/149‑1 (MQ=255) aaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAAAGATTAGAACTCTAGGATATTTTGCCATAGACGGTTTTGAAGACCGTTTGCTTTAAACACTCGGCCACGCTTCCAGCTCGtttt > 1:275329/1‑149 (MQ=25) cTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCGATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGgccgc > 6:292251/1‑148 (MQ=255) aGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGGCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGgccgccta > 7:61338/1‑146 (MQ=255) tattcaaaGCCCTGAGTATTCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGGCCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATAcc > 1:128987/6‑149 (MQ=255) aaaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGACCTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGGCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGTCCGCCATAATACCtt > 5:240855/1‑148 (MQ=255) aaaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCtt > 7:109941/1‑148 (MQ=255) aaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATg < 8:10731/149‑1 (MQ=255) aGCCCTGATTATTCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATg > 3:111261/1‑148 (MQ=255) aGCCCTGATTATTCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCAATCGGCCACCCTTCCAGCTCGTTTTGCGCCCGCAATAATACCTTAATg > 6:25494/1‑148 (MQ=38) gCCCTGATTATTCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAg > 6:110584/1‑116 (MQ=255) cTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAaca > 7:177822/1‑149 (MQ=255) cTGAATAATCAGGGCTGTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAaca > 6:226460/1‑149 (MQ=255) tGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAacac > 6:313003/1‑149 (MQ=255) | GAAAAGCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACAC > NC_002947/4501150‑4501442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |