Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 387,798 G→C 7.4% G313A (GGC→GCC)  glyA‑I → serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947387,7980GC 7.4% 248.9 / 8.9 81G313A (GGC→GCC) glyA‑Iserine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (33/42);  new base C (3/3);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

CACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGTCACTTCGGGCCTG  >  NC_002947/387659‑387946
                                                                                                                                           |                                                                                                                                                    
cACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACaa                                                                                                                                             >  1:336842/1‑149 (MQ=255)
 aCGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGCCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAac                                                                                                                                            >  3:94758/1‑149 (MQ=255)
   gTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAacca                                                                                                                                          <  2:139855/149‑1 (MQ=255)
       tCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTGTCGGTGCCACCGGAAaccacatc                                                                                                                                      <  4:128314/149‑4 (MQ=255)
         gCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGt                                                                                                                                     <  4:321517/148‑1 (MQ=255)
         gCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGt                                                                                                                                     >  3:321517/1‑148 (MQ=255)
          cTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTc                                                                                                                                   <  2:336842/149‑1 (MQ=255)
             ccAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTg                                                                                                                                <  5:242519/149‑1 (MQ=255)
              cAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTgg                                                                                                                               >  3:73241/1‑149 (MQ=255)
               aaGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTGTCGGTGCCACCGGAAaccacatcgta                                                                                                                                   <  1:8696/144‑7 (MQ=255)
               aaGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTGTCGGTGCCACCGGAAaccacatcgta                                                                                                                                   >  2:8696/1‑138 (MQ=255)
                aGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTc                                                                                                                             <  1:125353/149‑1 (MQ=255)
                aGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTc                                                                                                                             <  7:11670/149‑1 (MQ=255)
                        tgCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGa                                                                                                                      <  4:304615/148‑1 (MQ=255)
                              aaGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTACTGGTCAGCCTGATCCGt                                                                                                                 >  7:234602/1‑147 (MQ=255)
                              aaGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGCTTTCCGGTGGCACAGACAACCACCTGTTCATGGACAGCCTGATCCGt                                                                                                                 >  8:217625/1‑147 (MQ=255)
                               aGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTc                                                                                                                <  3:143694/147‑1 (MQ=255)
                               aGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTc                                                                                                                >  4:143694/1‑147 (MQ=255)
                                ggAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAgg                                                                                                             >  8:17995/1‑149 (MQ=255)
                                ggAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAgg                                                                                                             <  7:17995/149‑1 (MQ=255)
                                 gAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAggg                                                                                                            >  4:189410/1‑149 (MQ=255)
                                       cttgAGCCTGTATTGAAGGCCTACCAGCAGCAAGTGATCGAAATCGCCCAGGCCATGGCCCAGGTGTTCATCGACGGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACATGTTCGTGGTCAGCCTGATCCGTCAGGGCCTGAc                                                                                                      <  6:282982/146‑1 (MQ=255)
                                         cgAGCCTGGAATCAAGCCCTCCCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACAGTGGCTACGATGTGGTTTCCGGCGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTg                                                                                                    <  4:16702/148‑1 (MQ=255)
                                          gAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTgg                                                                                                   <  7:217625/149‑1 (MQ=255)
                                            gCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGc                                                                                                  >  5:313051/1‑148 (MQ=255)
                                                ggATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAacca                                                                                                                                          <  3:225719/104‑1 (MQ=255)
                                                ggATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAacca                                                                                                                                          >  4:225719/1‑104 (MQ=255)
                                                 gATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAgac                                                                                            <  7:116460/149‑1 (MQ=255)
                                                   ttCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAgacgc                                                                                          >  5:94396/1‑149 (MQ=255)
                                                     cAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAgacgcc                                                                                         <  7:168579/148‑1 (MQ=255)
                                                      aaGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                               <  2:39532/93‑1 (MQ=255)
                                                      aaGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGAc                                                                                                                                               >  1:39532/1‑93 (MQ=255)
                                                        ggCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAgacgccgacg                                                                                     >  5:314434/1‑149 (MQ=255)
                                                          ccTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTc                                                                                                                             <  4:147623/107‑1 (MQ=255)
                                                          ccTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTc                                                                                                                             >  3:147623/1‑107 (MQ=255)
                                                                agcagcAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTg                                                                             <  6:341337/149‑1 (MQ=255)
                                                                    gcaAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTc                                                                         >  8:282070/1‑149 (MQ=255)
                                                                     caAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTg                                                                             <  6:175970/144‑1 (MQ=255)
                                                                     caAGTGATAGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTg                                                                             >  5:175970/1‑144 (MQ=255)
                                                                       aGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTg                                                                      <  2:2753/149‑1 (MQ=255)
                                                                          gATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTgcg                                                                    >  1:151100/1‑148 (MQ=255)
                                                                           aTCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCTGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTTGGTCGTGCGCa                                                                  <  4:94758/149‑1 (MQ=255)
                                                                              gAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCata                                                                >  1:110393/1‑148 (MQ=255)
                                                                               aaaaCGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCatat                                                               >  5:251911/1‑148 (MQ=255)
                                                                                aaaCGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCa                                                             >  3:64549/1‑149 (MQ=255)
                                                                                    gCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCg                                                          >  7:299032/1‑148 (MQ=255)
                                                                                    gCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGt                                                         >  6:248448/1‑149 (MQ=255)
                                                                                     cccAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTc                                                        <  3:189410/149‑1 (MQ=255)
                                                                                      ccAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCaaa                                                                                               <  4:304215/109‑1 (MQ=255)
                                                                                      ccAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTca                                                       <  5:2624/149‑1 (MQ=255)
                                                                                       cAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTcaa                                                      >  5:253703/1‑149 (MQ=255)
                                                                                             aTGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGaa                                                <  6:313051/149‑1 (MQ=255)
                                                                                                  ccAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAggg                                                                                                            >  5:242788/1‑84 (MQ=255)
                                                                                                  ccAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAggg                                                                                                            <  6:242788/84‑1 (MQ=255)
                                                                                                   cAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGt                                          <  8:299032/149‑1 (MQ=255)
                                                                                                   cAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGt                                          >  1:72554/1‑149 (MQ=255)
                                                                                                    aGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTg                                                                                                                                >  2:90653/1‑62 (MQ=255)
                                                                                                    aGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTg                                                                                                                                <  1:90653/62‑1 (MQ=255)
                                                                                                     ggTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCTTGc                                        <  6:314434/149‑1 (MQ=255)
                                                                                                     ggTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGc                                        <  5:248448/149‑1 (MQ=255)
                                                                                                     ggTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGc                                        <  4:73241/149‑1 (MQ=255)
                                                                                                            aTCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTggg                                                                           <  6:169719/107‑1 (MQ=255)
                                                                                                            aTCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATGACCGTCAACAAGAAc                                               >  5:307248/1‑135 (MQ=255)
                                                                                                            aTCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAAc                                               <  6:307248/135‑1 (MQ=255)
                                                                                                                aCCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCg                             <  4:64549/149‑1 (MQ=255)
                                                                                                                aCCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCg                             <  2:110393/149‑1 (MQ=255)
                                                                                                                  cGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCa                           <  6:253703/149‑1 (MQ=255)
                                                                                                                    tGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGaa                                    >  8:12507/1‑138 (MQ=255)
                                                                                                                    tGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGaa                                    <  7:12507/138‑1 (MQ=255)
                                                                                                                       cTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGc                      <  5:285343/149‑1 (MQ=255)
                                                                                                                         aacaggtgGTTGTCGGTGCCACCGGAAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCg                    >  6:82916/6‑149 (MQ=255)
                                                                                                                           caggtgGTTGTCGGTGCCACCGGAAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGtt                  >  8:284030/4‑149 (MQ=255)
                                                                                                                             tgtgGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCg                                                          >  1:116062/1‑107 (MQ=255)
                                                                                                                             tgtgGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCg                                                          <  2:116062/107‑1 (MQ=255)
                                                                                                                              gtgGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGt               >  4:90447/1‑149 (MQ=255)
                                                                                                                              gtgGTTGTCGGTGCCACCGGAAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCAcc                                                           <  6:267281/105‑1 (MQ=39)
                                                                                                                                     ccGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTcaac                                                     <  7:305274/104‑1 (MQ=255)
                                                                                                                                     ccGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTcaac                                                     >  8:305274/1‑104 (MQ=255)
                                                                                                                                      cGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGTCACTTCgg       <  8:234602/149‑1 (MQ=255)
                                                                                                                                      cGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGTCACTTCgg       <  2:151100/149‑1 (MQ=255)
                                                                                                                                        gTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGTCACTTCGGGc     >  3:156893/1‑149 (MQ=255)
                                                                                                                                           gCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGTCACTTCGGGCCTg  <  2:72554/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
CACGTGATCGCTGCCAAGGCAGTGTGCTTCAAGGAAGCGCTGGAGCCTGGATTCAAGGCCTACCAGCAGCAAGTGATCGAAAACGCCCAGGCCATGGCCCAGGTGTTCATCGACCGTGGCTACGATGTGGTTTCCGGTGGCACCGACAACCACCTGTTCCTGGTCAGCCTGATCCGTCAGGGCCTGACTGGCAAAGACGCCGACGCCGCCCTGGGTCGTGCGCATATCACCGTCAACAAGAACGCCGTGCCGAACGACCCGCAGTCGCCGTTCGTCACTTCGGGCCTG  >  NC_002947/387659‑387946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑