Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,520,454 A→T 14.3% intergenic (+45/+27) cspD → / ← icd DNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,520,4540AT14.3% 160.1 / 19.1 70intergenic (+45/+27)cspD/icdDNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base T (6/4);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01

CCCCAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCTT  >  NC_002947/4520307‑4520600
                                                                                                                                                   |                                                                                                                                                  
ccccAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaa                                                                                                                                                   <  7:30117/149‑1 (MQ=255)
 cccAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTaaa                                                                                                                                                  <  3:284631/149‑1 (MQ=255)
     aGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAACTCGGCCGCTTCTTTAAaagaa                                                                                                                                              <  2:194068/149‑1 (MQ=255)
      gggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAaagaag                                                                                                                                             <  6:143880/149‑1 (MQ=255)
      gggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAaagaag                                                                                                                                             <  8:193256/149‑1 (MQ=255)
       ggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGATGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGc                                                                                                                                            <  3:337758/149‑1 (MQ=255)
       ggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGc                                                                                                                                            <  1:302381/149‑1 (MQ=255)
         cTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTTAAAGAAGCgg                                                                                                                                          <  6:229150/149‑2 (MQ=255)
                   gTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCt                                                                                                                                <  3:9335/149‑1 (MQ=255)
                    ttGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAACCGGTCGCTTCTTTTAAAGAAGCGGCCGAttttttt                                                                                                                               <  7:149490/149‑4 (MQ=38)
                     tGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGGTTTCTGt                                                                                                                              >  7:238630/1‑149 (MQ=255)
                       aaaTCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTg                                                                                                                            >  3:80211/1‑149 (MQ=255)
                         aTCAAAGATGCCGCGGCCGCTGCTGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGcc                                                                                                                          <  8:191579/149‑1 (MQ=255)
                          tCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCt                                                                                                                         <  2:116533/149‑1 (MQ=255)
                             cctctGCCGCAGCGGCCGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTa                                                                                                                       >  5:43939/5‑148 (MQ=255)
                                 tGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAACCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACAtgt                                                                                                                  <  5:170196/149‑1 (MQ=255)
                                  gCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGAc                                                                                                                                         <  5:76566/125‑1 (MQ=255)
                                  gCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGAc                                                                                                                                         >  6:76566/1‑125 (MQ=255)
                                   ccGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATgtgt                                                                                                                >  5:131151/1‑149 (MQ=255)
                                      cgGCCGCTGCGTCAGATGGTGCAAGAGCCTTTGGCGAGCCGCAAATCGAAACTGCAAAAGCCTGACTGCATTCGATGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTg                                                                                                             <  1:311988/149‑1 (MQ=255)
                                                 gCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATc                                                                                                  >  8:196067/1‑149 (MQ=255)
                                                       gCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCa                                                                                              >  5:73863/1‑147 (MQ=255)
                                                       gCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCa                                                                                              >  5:244078/1‑147 (MQ=255)
                                                         tGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGc                                                                                          >  4:113309/1‑149 (MQ=255)
                                                          gCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGcc                                                                                         >  3:243866/1‑149 (MQ=255)
                                                                       gCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTAc                                                                             >  7:201425/1‑148 (MQ=255)
                                                                       gCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTAc                                                                             <  3:113309/148‑1 (MQ=255)
                                                                         gAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAg                                                                          >  1:108268/1‑149 (MQ=255)
                                                                             cGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTg                                                                      <  2:108268/149‑1 (MQ=255)
                                                                              gCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTgg                                                                     <  6:244078/149‑1 (MQ=255)
                                                                               cAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGTCGCTTCTTTTAAAGAAGCGGCCGAttttttt                                                                                                                               >  1:202283/1‑87 (MQ=25)
                                                                               cAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGTCGCTTCTTTTAAAGAAGCGGCCGAttttttt                                                                                                                               <  2:202283/90‑4 (MQ=25)
                                                                                   gCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGccc                                                                >  8:102728/1‑149 (MQ=255)
                                                                                    cGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCt                                                               <  4:80211/149‑1 (MQ=255)
                                                                                     gAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTc                                                              >  1:195647/1‑149 (MQ=255)
                                                                                            cAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCaa                                                        <  2:195647/148‑1 (MQ=255)
                                                                                             aaaaGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATc                                                      >  1:214585/1‑149 (MQ=255)
                                                                                                 gCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTc                                                  >  1:340505/1‑149 (MQ=255)
                                                                                                    tGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGa                                                >  1:4872/1‑148 (MQ=255)
                                                                                                         gcctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCg                                           >  5:59531/1‑148 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATc                                            >  4:227977/1‑146 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATc                                            <  3:227977/146‑1 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCg                                           >  7:45726/1‑147 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         <  4:243866/149‑1 (MQ=255)
                                                                                                          cctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         <  7:102728/149‑1 (MQ=255)
                                                                                                           ctgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAg                                        <  2:214585/149‑1 (MQ=255)
                                                                                                            tgcGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAgg                                       >  2:157611/1‑149 (MQ=255)
                                                                                                                            aCAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCg                        >  7:112949/1‑148 (MQ=255)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         <  5:300213/129‑1 (MQ=255)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         >  6:300213/1‑129 (MQ=255)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCaa                     <  2:4872/149‑1 (MQ=255)
                                                                                                                              aaaaaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCaa                     >  2:275881/1‑149 (MQ=255)
                                                                                                                                 aaaaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTACTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATgg                  >  1:229947/1‑149 (MQ=255)
                                                                                                                                   aaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGgc                                                                    <  5:163806/97‑1 (MQ=255)
                                                                                                                                   aaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGgc                                                                    >  6:163806/1‑97 (MQ=255)
                                                                                                                                   aaTCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGgcg                >  7:244701/1‑149 (MQ=255)
                                                                                                                                      aggtcGCTTGTTTTAAAGAAGCGGCCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATGGCCAAAGCGGGAGCTGCTTACCAGCTTGGCGCCCTCAATCAAGCGCTCGAAATCGTAGGTCACGCGCTTGGCCGCAATGGCGCCa             >  8:320342/5‑149 (MQ=255)
                                                                                                                                       ggtcGCTTCTTTTAAAGAAGCGGCCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCAt            >  5:160390/4‑149 (MQ=255)
                                                                                                                                       ggtcGCTTCTTTTAAAGAAGCGGCCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCAt            >  7:276040/4‑149 (MQ=255)
                                                                                                                                       ggCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCAt            <  6:43939/149‑1 (MQ=255)
                                                                                                                                        gtcGCTTCTTTTAAAGAAGCGGCCGGTTTTCTGTTGCCTTACATGTGTTTGATCTTTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         >  2:340478/3‑119 (MQ=255)
                                                                                                                                        gtcGCTTCTTTTAAAGAAGCGGCCGGTTTTCTGTTGCCTTACATGTGTTTGATCTTTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTa                                         <  1:340478/117‑1 (MQ=255)
                                                                                                                                         tcGCTTCTTTTAAAGAAGCGGCCGATTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTg          >  4:75471/2‑149 (MQ=255)
                                                                                                                                         ccGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTg          <  2:340505/149‑1 (MQ=255)
                                                                                                                                         ccGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTg          <  8:244701/149‑1 (MQ=255)
                                                                                                                                               cttTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACg                                  >  6:49293/1‑119 (MQ=255)
                                                                                                                                               cttTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACg                                  <  5:49293/119‑1 (MQ=255)
                                                                                                                                               cttTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGcc     <  8:45726/148‑1 (MQ=255)
                                                                                                                                                tttAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCt   >  1:268168/1‑149 (MQ=255)
                                                                                                                                                 ttAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCtt  >  5:304773/1‑149 (MQ=255)
                                                                                                                                                 ttAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCtt  >  4:307082/1‑149 (MQ=255)
                                                                                                                                                  tAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCtt  <  7:196067/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCCAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTAAAAGAAGCGACCGGTTTTCTGTTGCCTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCTT  >  NC_002947/4520307‑4520600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑