Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,530,025 T→A 18.0% intergenic (+31/‑137) PP_4018 → / → topB acetyltransferase/DNA topoisomerase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,530,0250TA18.0% 79.3 / 12.2 39intergenic (+31/‑137)PP_4018/topBacetyltransferase/DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (18/14);  new base A (3/4);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

CCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATG  >  NC_002947/4529883‑4530164
                                                                                                                                              |                                                                                                                                           
ccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCcg                                                                                                                                       >  4:198444/1‑149 (MQ=255)
ccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCcg                                                                                                                                       >  2:107479/1‑149 (MQ=255)
    gTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGaa                                                                                                                                   <  6:231843/149‑1 (MQ=255)
    gTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGaa                                                                                                                                   <  7:21549/149‑1 (MQ=255)
      gCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAaga                                                                                                                                 <  8:79233/149‑1 (MQ=255)
       cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAaga                                                                                                                                 <  3:10959/148‑1 (MQ=255)
         cgccacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAAGAgg                                                                                                                               <  4:256983/148‑1 (MQ=255)
         cgccacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAAGAgg                                                                                                                               >  3:256983/1‑148 (MQ=255)
          gccacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGcc                                                                                                                             >  5:98816/1‑149 (MQ=255)
            cacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCgg                                                                                                                           >  5:348298/1‑149 (MQ=255)
               gccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCt                                                                                                                         >  5:79997/1‑148 (MQ=255)
                    tCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGc                                                                                                                    >  5:342900/1‑148 (MQ=255)
                                     tttCGCGTAGTCAGCGAGTAATTCCTCTATGCTGGCATCCCGCATGGCTACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTCCCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGa                                                                                                  <  6:79997/149‑1 (MQ=255)
                                                 aGCGAGGATTTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATTACCTGCACTGGCATCATGGCTTGTTTTCCCGCGAATAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCt                                                                                        <  5:239628/147‑1 (MQ=255)
                                                 aGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTg                                                                                       >  8:55079/1‑148 (MQ=255)
                                                   cGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCAGTa                                                                                     >  5:316354/1‑148 (MQ=255)
                                                            ccTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACatccatcc                                                                            <  7:55079/148‑1 (MQ=255)
                                                             cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGCGGCCGGCTCAGGCGata                                                                                                                >  5:174563/1‑111 (MQ=255)
                                                             cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATAAATACCCGCTGACAAAAAACCTGTAcatacatc                                                                             >  4:307869/1‑146 (MQ=255)
                                                             cTCGAAGCTGCCATCCCGCATGTCCACATGGTGCGCGACTCGCGCGCCTTATCCATCACCTGCACTGGCCTCTTCGCGGTTTAACCCGCTAAGAGGCCGGCTCAGGCGata                                                                                                                <  6:174563/111‑1 (MQ=255)
                                                                  aGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACAtccatcca                                                                           >  3:249622/1‑143 (MQ=255)
                                                                  aGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACAtccatcca                                                                           <  4:249622/143‑1 (MQ=255)
                                                                      ggCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatcc                                                                                >  1:200707/1‑134 (MQ=255)
                                                                       gCATCCCGCATGCCGACATGGGGCGCGACGCGCGCGCCTGACCCATCACCGGCACTGGCCTCTTCGCGGGTTTCCCCGCGATGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACtt                                                                 <  6:188420/148‑1 (MQ=255)
                                                                        cATCCCGCTTGTCCAACTGGGGCGCGACTCGCTCGCCTGATCCATCACGTTCAGTGCCCTCTTCGCGGGTTTACCCGCTATGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGc                                                               <  6:257087/149‑1 (MQ=255)
                                                                        cATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGc                                                               <  6:348298/149‑1 (MQ=255)
                                                                         aTCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACtt                                                                 <  1:235235/146‑1 (MQ=255)
                                                                         aTCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACtt                                                                 >  2:235235/1‑146 (MQ=255)
                                                                          tCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTAGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATAAATGCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTggca                                                             >  3:338867/1‑146 (MQ=255)
                                                                            ccGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCctct                                                           >  1:130307/1‑149 (MQ=255)
                                                                                           gTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc                                             <  6:342900/148‑1 (MQ=255)
                                                                                           gTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGGTTACCCGCGCAGAGGCCGGCTCAGGCGATATACAACACCGCTGACAAAAAACCTGTACATCCATACAGATAAAACTTGCCTCTGCGgcccccggtgcg                                            >  4:194918/1‑142 (MQ=255)
                                                                                                                   aTCACCTTCACTGGCTTCTTCGGGTTTTTACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt                    <  2:130307/149‑1 (MQ=255)
                                                                                                                   aTCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt                    >  3:164765/1‑149 (MQ=255)
                                                                                                                        cTGCATTGGCCTTTTCGGGGTTTAACCCGGTATGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGc               <  3:307869/149‑1 (MQ=255)
                                                                                                                                  ctctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc                                             >  1:2112/1‑109 (MQ=255)
                                                                                                                                  ctctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc                                             <  2:2112/109‑1 (MQ=255)
                                                                                                                                   tctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCATGCGCATCCTATGGCCCACCACCCCGATGAAGCGAACCCGGCCa    >  1:116565/1‑149 (MQ=255)
                                                                                                                                     ttCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg  >  3:102311/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATG  >  NC_002947/4529883‑4530164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑