Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,532,984 T→C 7.7% L239P (CTG→CCG)  PP_4020 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,532,9840TC 7.7% 147.6 / 3.0 52L239P (CTG→CCG) PP_4020oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base C (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CTGAAACTGGCACAGGGCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGCGTGATCGACAAC  >  NC_002947/4532836‑4533129
                                                                                                                                                     |                                                                                                                                                 
cTGAAACTGGCACAGGGCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCt                                                                                                                                                   >  3:215870/1‑149 (MQ=255)
 tggtaCTGGC‑CAGGCCCTGTGCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTg                                                                                                                                                  >  8:187935/5‑149 (MQ=255)
           acaGGGCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACg                                                                                                                                        >  5:131478/1‑149 (MQ=255)
           acaGGGCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACg                                                                                                                                        >  5:184790/1‑149 (MQ=255)
               ggCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTa                                                                                                                                    <  6:131478/149‑1 (MQ=255)
                gCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTCCTGGCCTGCAACGCCTa                                                                                                                                    >  7:140902/1‑148 (MQ=255)
                  cTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACat                                                                                                                                 >  6:3916/1‑149 (MQ=255)
                   tG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACatc                                                                                                                                >  3:181939/1‑149 (MQ=255)
                      gCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACatc                                                                                                                                <  2:97313/147‑1 (MQ=255)
                       ccAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACatcgat                                                                                                                             <  6:184790/149‑1 (MQ=255)
                        cAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACatcgatc                                                                                                                            <  1:40019/149‑1 (MQ=255)
                           tACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGcc                                                                                                                         <  4:50945/149‑1 (MQ=255)
                              cATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCtt                                                                                                                       >  3:135661/1‑148 (MQ=255)
                                       tGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTCCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGcc                                                                                                             >  3:136572/1‑149 (MQ=255)
                                             cggGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTcgc                                                                                                       <  5:3916/149‑1 (MQ=255)
                                                gCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTcg                                                                                                        >  6:85594/1‑145 (MQ=255)
                                                gCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTcg                                                                                                        <  5:85594/145‑1 (MQ=255)
                                                       tACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGtt                                                                                             <  4:135661/149‑1 (MQ=255)
                                                                     ggTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTcc                                                                                >  8:309364/1‑148 (MQ=255)
                                                                      gTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTa                                                                              <  4:181939/149‑1 (MQ=255)
                                                                           cAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAgg                                                                         >  5:10366/1‑149 (MQ=255)
                                                                            aGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTAc                                                                             >  6:316645/1‑144 (MQ=255)
                                                                            aGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTAc                                                                             <  5:316645/144‑1 (MQ=255)
                                                                            aGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGt                                                                        >  3:109110/1‑149 (MQ=255)
                                                                            aGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGGCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAg                                                                          <  3:94061/147‑1 (MQ=255)
                                                                             gCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTc                                                                       <  4:109110/149‑1 (MQ=255)
                                                                                  cagcagGCTCGCGACGGCTATGTCGCGCGTAGCGACAGTTGCGAAGTTCGCTGGCAGGTATTGGTTCTGGCCTGCAACGCCTACATAGATCGCCTTGTCCGGGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCGTACCAGGTCGCCAc                                                                  <  8:140902/149‑1 (MQ=255)
                                                                                        gCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGcc                                                            >  4:15191/1‑149 (MQ=255)
                                                                                            gcgACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCctct                                                         <  7:187935/148‑1 (MQ=255)
                                                                                              gACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGa                                                      >  6:223094/1‑149 (MQ=255)
                                                                                                  gCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGcc                                                  <  4:136572/149‑1 (MQ=255)
                                                                                                       gTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACtt                                             >  7:166820/1‑149 (MQ=255)
                                                                                                         cGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTc                                                                                 >  2:119290/1‑111 (MQ=255)
                                                                                                               tACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCAtt                                     <  6:10366/149‑1 (MQ=255)
                                                                                                                  cgaccgTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGt                                  >  2:241530/1‑149 (MQ=255)
                                                                                                                   gaccgTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGt                                  >  8:219991/1‑148 (MQ=255)
                                                                                                                        tGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTtactac                            >  6:271745/1‑149 (MQ=255)
                                                                                                                            gAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCg                        >  1:314540/1‑149 (MQ=255)
                                                                                                                               gaacttcgCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCaa                     <  5:245087/142‑1 (MQ=255)
                                                                                                                                ttCGCTGCCAGGTATTGGTACTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTAGCCCATTCGTTACTACCTCGCaa                     >  3:251947/1‑148 (MQ=255)
                                                                                                                                    cTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGtt                <  7:219991/149‑1 (MQ=255)
                                                                                                                                    cTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCCCGCAACAGtt                >  1:160520/1‑149 (MQ=255)
                                                                                                                                       ccAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGc              <  7:309364/148‑1 (MQ=255)
                                                                                                                                           gTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGCGTGAt         <  8:166820/149‑1 (MQ=255)
                                                                                                                                           gTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGCGTGAt         >  1:229713/1‑149 (MQ=255)
                                                                                                                                           gTATTGGTGCTGGCCTGCAAAGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGCGTGAt         >  6:301830/1‑149 (MQ=255)
                                                                                                                                           gTATTGGTGCCGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGc              <  6:50972/144‑1 (MQ=255)
                                                                                                                                           gTATTGGTGCCGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGc              <  6:45837/144‑1 (MQ=255)
                                                                                                                                           gTATTGGTGCCGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGc              >  5:45837/1‑144 (MQ=255)
                                                                                                                                           gTATTGGTGCCGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGACTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGc              >  5:50972/1‑144 (MQ=255)
                                                                                                                                                  tGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGCGTGATCGACAAc  <  4:315439/149‑1 (MQ=255)
                                                                                                                                                    cTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGc                                          >  6:220693/1‑107 (MQ=255)
                                                                                                                                                    cTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGc                                          <  5:220693/107‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CTGAAACTGGCACAGGGCCTG‑GCCAGTACCATCGAGGCTGCCGGCGGGCAAATCTACGAACAGAGCCAGGTGCTCAGCTACCAGCAGGCTCGCGACGGCTATGTCGCCCGTACCGACCGTGGCGAAGTTCGCTGCCAGGTATTGGTGCTGGCCTGCAACGCCTACATCGATCGCCTTGACCGCGGCCTGTCGCGCCGCCTGTTGCCCGTCGGTTCCTACCAGGTCGCCACTGCGCCTCTGGATGCCGACTTCGCCCATTCGTTACTACCTCGCAACAGTTGCGTGATCGACAAC  >  NC_002947/4532836‑4533129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑