Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,597,751 C→A 8.7% Q686H (CAG→CAT) ‡ PP_4071 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,597,7510CA 8.7% 142.0 / 3.9 46Q686H (CAG→CAT) ‡PP_4071membrane protein
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

TTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTT  >  NC_002947/4597605‑4597893
                                                                                                                                                  |                                                                                                                                               
ttGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCctgctg                                                                                                                                               >  1:102594/1‑149 (MQ=255)
  gAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCtgctgc                                                                                                                                              <  8:8707/148‑1 (MQ=255)
     gCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCTCTGGCTCGCGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAAGTGCTCCTGGGCCTGCTGCTTCa                                                                                                                                          <  7:51547/149‑1 (MQ=255)
           ggCTGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACGTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGccc                                                                                                                                     <  7:154607/148‑1 (MQ=255)
            gCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCg                                                                                                                                    <  2:102594/148‑1 (MQ=255)
              aGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGCACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCa                                                                                                                                  >  7:286562/1‑148 (MQ=255)
              aGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCAt                                                                                                                                 <  2:345063/149‑1 (MQ=255)
                         gcgcCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                      <  7:17565/149‑1 (MQ=255)
                         gcgcCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                      <  1:341161/149‑1 (MQ=255)
                                 aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                      >  4:132522/1‑141 (MQ=255)
                                 aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                      <  3:132522/141‑1 (MQ=255)
                                            gTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGc                                                                                                   >  4:187271/1‑148 (MQ=255)
                                                 ccTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTc                                                                                             <  1:252072/149‑1 (MQ=255)
                                                       tCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTctgctg                                                                                        >  5:288419/1‑148 (MQ=255)
                                                         gAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACAGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCAc                                                                                     >  1:149245/1‑149 (MQ=255)
                                                           gCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCAACTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGt                                                                                   <  8:43781/149‑1 (MQ=255)
                                                           gCCGGAAGACAGCTGGTAAACGACATCGAACACAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                 >  8:43378/1‑100 (MQ=255)
                                                           gCCGGAAGACAGCTGGTAAACGACATCGAACACAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                 <  7:43378/104‑5 (MQ=255)
                                                                 aGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGa                                                                             <  3:187271/149‑1 (MQ=255)
                                                                        tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTg                                                                      >  7:248112/1‑149 (MQ=255)
                                                                          gTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTg                                                                      <  2:149245/147‑1 (MQ=255)
                                                                              aCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATCCACGCGTTGTGGCAACGGC‑ACCTTACGCAAGGTCTGCTGCACGGTGCTGCGACGCGGGCgct                                                                 >  6:239857/1‑146 (MQ=255)
                                                                              aCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGAGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATa                                                                >  2:81275/1‑149 (MQ=255)
                                                                                   aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                     >  6:88370/1‑139 (MQ=255)
                                                                                   aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                     <  5:88370/139‑1 (MQ=255)
                                                                                   aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCCTTGTGGCAACGGC‑ACCTTACGCAATCTCTGCTGCACGTGGCTGACACCCTGGCGATACTGCg                                                           >  6:213489/1‑149 (MQ=255)
                                                                                    tCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGGAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCTACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCg                                                           <  1:81275/149‑1 (MQ=255)
                                                                                           cAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                   <  5:60894/149‑1 (MQ=255)
                                                                                            aGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCgg                                                  >  2:69373/1‑149 (MQ=255)
                                                                                                cccTCCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCtgctgc                                                                                       <  6:134435/108‑1 (MQ=255)
                                                                                                cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCtgctgc                                                                                       >  5:134435/1‑108 (MQ=255)
                                                                                                cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATc                                              >  8:145961/1‑149 (MQ=255)
                                                                                                  cACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCAc                                                                                     <  4:13986/108‑1 (MQ=255)
                                                                                                  cACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCAc                                                                                     >  3:13986/1‑108 (MQ=255)
                                                                                                    ccTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCcgcg                                          >  7:42293/1‑149 (MQ=255)
                                                                                                             gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCTCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTgg                                                                                 >  8:255992/1‑101 (MQ=255)
                                                                                                             gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCTCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTgg                                                                                 <  7:255992/101‑1 (MQ=255)
                                                                                                                  tCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATa                            <  6:288419/149‑1 (MQ=255)
                                                                                                                     aGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACtt                         <  7:145961/149‑1 (MQ=255)
                                                                                                                             aTGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGcc                 >  1:144658/1‑149 (MQ=255)
                                                                                                                                cAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCa              <  1:162481/149‑1 (MQ=255)
                                                                                                                                cAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCa              >  2:162481/1‑149 (MQ=255)
                                                                                                                                 aaCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                 >  6:62976/1‑129 (MQ=255)
                                                                                                                                 aaCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                 <  5:62976/129‑1 (MQ=255)
                                                                                                                                         cTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc     <  1:69373/149‑1 (MQ=255)
                                                                                                                                         cTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc     >  7:281385/1‑149 (MQ=255)
                                                                                                                                           gggCCTGAAGCAGCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGt   >  2:12919/1‑149 (MQ=255)
                                                                                                                                            ggCCTGCTGCTTCAGGCCCTCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCGGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTGGtt  <  5:213489/149‑1 (MQ=255)
                                                                                                                                                 gctgctTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTg                                                             >  2:132055/1‑85 (MQ=255)
                                                                                                                                                 gctgctTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTg                                                             <  1:132055/85‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTT  >  NC_002947/4597605‑4597893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑