Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,597,755 T→A 8.7% K685M (AAG→ATG) ‡ PP_4071 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,597,7550TA 8.7% 125.6 / 3.7 46K685M (AAG→ATG) ‡PP_4071membrane protein
Reads supporting (aligned to +/- strand):  ref base T (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGC  >  NC_002947/4597610‑4597901
                                                                                                                                                 |                                                                                                                                                   
gCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCTCTGGCTCGCGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAAGTGCTCCTGGGCCTGCTGCTTCa                                                                                                                                                  <  7:51547/149‑1 (MQ=255)
      ggCTGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACGTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGccc                                                                                                                                             <  7:154607/148‑1 (MQ=255)
       gCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCg                                                                                                                                            <  2:102594/148‑1 (MQ=255)
         aGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGCACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCa                                                                                                                                          >  7:286562/1‑148 (MQ=255)
         aGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCAt                                                                                                                                         <  2:345063/149‑1 (MQ=255)
                    gcgcCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                              <  1:341161/149‑1 (MQ=255)
                    gcgcCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                              <  7:17565/149‑1 (MQ=255)
                            aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                              >  4:132522/1‑141 (MQ=255)
                            aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                              <  3:132522/141‑1 (MQ=255)
                                       gTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGc                                                                                                           >  4:187271/1‑148 (MQ=255)
                                            ccTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTc                                                                                                     <  1:252072/149‑1 (MQ=255)
                                                  tCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTctgctg                                                                                                >  5:288419/1‑148 (MQ=255)
                                                    gAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACAGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCAc                                                                                             >  1:149245/1‑149 (MQ=255)
                                                      gCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCAACTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGt                                                                                           <  8:43781/149‑1 (MQ=255)
                                                      gCCGGAAGACAGCTGGTAAACGACATCGAACACAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                         >  8:43378/1‑100 (MQ=255)
                                                      gCCGGAAGACAGCTGGTAAACGACATCGAACACAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                         <  7:43378/104‑5 (MQ=255)
                                                            aGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGa                                                                                     <  3:187271/149‑1 (MQ=255)
                                                                   tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTg                                                                              >  7:248112/1‑149 (MQ=255)
                                                                     gTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTg                                                                              <  2:149245/147‑1 (MQ=255)
                                                                         aCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATCCACGCGTTGTGGCAACGGC‑ACCTTACGCAAGGTCTGCTGCACGGTGCTGCGACGCGGGCgct                                                                         >  6:239857/1‑146 (MQ=255)
                                                                         aCGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGAGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATa                                                                        >  2:81275/1‑149 (MQ=255)
                                                                              aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                             >  6:88370/1‑139 (MQ=255)
                                                                              aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                             <  5:88370/139‑1 (MQ=255)
                                                                              aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCCTTGTGGCAACGGC‑ACCTTACGCAATCTCTGCTGCACGTGGCTGACACCCTGGCGATACTGCg                                                                   >  6:213489/1‑149 (MQ=255)
                                                                               tCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGGAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCTACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCg                                                                   <  1:81275/149‑1 (MQ=255)
                                                                                      cAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                           <  5:60894/149‑1 (MQ=255)
                                                                                       aGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCgg                                                          >  2:69373/1‑149 (MQ=255)
                                                                                           cccTCCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCtgctgc                                                                                               <  6:134435/108‑1 (MQ=255)
                                                                                           cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCtgctgc                                                                                               >  5:134435/1‑108 (MQ=255)
                                                                                           cccACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATc                                                      >  8:145961/1‑149 (MQ=255)
                                                                                             cACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCAc                                                                                             <  4:13986/108‑1 (MQ=255)
                                                                                             cACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCAc                                                                                             >  3:13986/1‑108 (MQ=255)
                                                                                               ccTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCcgcg                                                  >  7:42293/1‑149 (MQ=255)
                                                                                                        gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCTCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTgg                                                                                         >  8:255992/1‑101 (MQ=255)
                                                                                                        gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCTCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTgg                                                                                         <  7:255992/101‑1 (MQ=255)
                                                                                                             tCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATa                                    <  6:288419/149‑1 (MQ=255)
                                                                                                                aGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACtt                                 <  7:145961/149‑1 (MQ=255)
                                                                                                                        aTGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGcc                         >  1:144658/1‑149 (MQ=255)
                                                                                                                           cAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCa                      <  1:162481/149‑1 (MQ=255)
                                                                                                                           cAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCa                      >  2:162481/1‑149 (MQ=255)
                                                                                                                            aaCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                         >  6:62976/1‑129 (MQ=255)
                                                                                                                            aaCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                         <  5:62976/129‑1 (MQ=255)
                                                                                                                                    cTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc             <  1:69373/149‑1 (MQ=255)
                                                                                                                                    cTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc             >  7:281385/1‑149 (MQ=255)
                                                                                                                                      gggCCTGAAGCAGCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGt           >  2:12919/1‑149 (MQ=255)
                                                                                                                                       ggCCTGCTGCTTCAGGCCCTCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCGGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTGGtt          <  5:213489/149‑1 (MQ=255)
                                                                                                                                            gctgctTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTg                                                                     >  2:132055/1‑85 (MQ=255)
                                                                                                                                            gctgctTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTg                                                                     <  1:132055/85‑1 (MQ=255)
                                                                                                                                                ctTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGc  <  8:248112/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGC‑ACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAGCTGC  >  NC_002947/4597610‑4597901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑