Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,634,359 A→T 10.2% V251D (GTC→GAC)  uvrC ← UvrABC system protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,634,3590AT10.2% 129.3 / 5.7 49V251D (GTC→GAC) uvrCUvrABC system protein C
Reads supporting (aligned to +/- strand):  ref base A (23/21);  new base T (2/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

GAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGC  >  NC_002947/4634217‑4634506
                                                                                                                                              |                                                                                                                                                   
gAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACGTCATcg                                                                                                                                                >  1:322919/1‑148 (MQ=255)
gAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACGTCATcg                                                                                                                                                <  2:322919/148‑1 (MQ=255)
     cGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACGTCATcgccgcct                                                                                                                                          <  7:216999/149‑3 (MQ=255)
       gCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTgcc                                                                                                                                        <  4:43019/149‑1 (MQ=255)
                        ctcctcctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGtt                                                                                                                       <  3:237654/149‑1 (MQ=255)
                         tcctcctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTAc                                                                                                                           <  8:56934/144‑1 (MQ=255)
                         tcctcctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTAc                                                                                                                           >  7:56934/1‑144 (MQ=255)
                             cctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTcc                                                                                                                  >  7:63107/1‑149 (MQ=255)
                              ctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTc                                                                                                                   <  5:262423/147‑1 (MQ=255)
                                      ccACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCt                                                                                                                 <  8:308151/141‑1 (MQ=255)
                                      ccACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCt                                                                                                                 >  7:308151/1‑141 (MQ=255)
                                              gggAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGcaacaa                                                                                                 <  2:133699/149‑1 (MQ=255)
                                                      gTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGATGGCGGCGATGACGGCGACATCGCCGCTGCCGCCCTCGATGTACGGTTGGTCCTGCGCGCGGCGGAACACGGCGctct                                                                                         >  8:285863/1‑149 (MQ=255)
                                                      gTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACGTCATcgccgc                                                                                                                                            <  1:221393/98‑1 (MQ=255)
                                                      gTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACGTCATcgccgc                                                                                                                                            >  2:221393/1‑98 (MQ=255)
                                                      gTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCt                                                                                         >  6:72977/1‑149 (MQ=255)
                                                                cccAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGcaac                                                                                                   <  3:51231/129‑1 (MQ=255)
                                                                cccAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGcaac                                                                                                   >  4:51231/1‑129 (MQ=255)
                                                                     tACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTcgg                                                                          >  2:312717/1‑149 (MQ=255)
                                                                       cTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTcggcg                                                                        >  3:279925/1‑149 (MQ=255)
                                                                          gcccgccGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATTTACTGt                                                                                                                        <  6:24595/98‑1 (MQ=255)
                                                                          gcccgccGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATTTACTGt                                                                                                                        >  5:24595/1‑98 (MQ=255)
                                                                            ccgccgCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCttt                                                                   <  4:251092/149‑1 (MQ=255)
                                                                              gccgcGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCttttt                                                                 >  8:16613/1‑149 (MQ=255)
                                                                               ccgcGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTc                                                                <  8:63107/149‑1 (MQ=255)
                                                                                 gcgcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCt                                                                     <  5:12662/142‑1 (MQ=255)
                                                                                 gcgcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCt                                                                     >  6:12662/1‑142 (MQ=255)
                                                                                 gcgcACGCTGATCAGCTGCACACAGCCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGa                                                              <  6:210763/149‑1 (MQ=255)
                                                                                  cgcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGa                                                              >  4:59931/1‑148 (MQ=255)
                                                                                    cACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAAt                                                           <  7:16613/149‑1 (MQ=255)
                                                                                     aCGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATc                                                          >  2:96946/1‑149 (MQ=255)
                                                                                          gATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAAGAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAggg                                                     >  3:245701/1‑149 (MQ=255)
                                                                                          gATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAggg                                                     >  2:27764/1‑149 (MQ=255)
                                                                                           aTCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGc                                                    >  8:306492/1‑149 (MQ=255)
                                                                                               ggTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATg                                                <  3:59931/149‑1 (MQ=255)
                                                                                                gTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAg                                                       <  1:1803/141‑1 (MQ=255)
                                                                                                gTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAg                                                       >  2:1803/1‑141 (MQ=255)
                                                                                                   cacacaGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATggcgg                                            >  8:263947/1‑149 (MQ=255)
                                                                                                      acaGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCt                                         >  4:149044/1‑149 (MQ=255)
                                                                                                      acaGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCt                                         >  3:30189/1‑149 (MQ=255)
                                                                                                        aGGCACCACCCGGGTTGCCGAAGGCGGCTATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCttt                                       <  7:285863/149‑1 (MQ=255)
                                                                                                                 ccGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTcc                                    >  6:165144/1‑143 (MQ=255)
                                                                                                                 ccGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTcc                                    <  5:165144/143‑1 (MQ=255)
                                                                                                                    ggTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCg                           <  5:65285/149‑1 (MQ=255)
                                                                                                                                cggcgATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTg               <  5:72977/149‑1 (MQ=255)
                                                                                                                                           tCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAAGAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTg    <  4:245701/149‑1 (MQ=255)
                                                                                                                                           tCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTg    >  8:287557/1‑149 (MQ=255)
                                                                                                                                             gACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTgg   <  3:149044/148‑1 (MQ=255)
                                                                                                                                             gACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGc  >  6:236185/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTGGC  >  NC_002947/4634217‑4634506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑