Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,649,982 T→G 15.1% H311P (CAC→CCC)  PP_4113 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,649,9820TG15.1% 132.0 / 14.8 53H311P (CAC→CCC) PP_4113hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base G (3/5);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TTGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCC  >  NC_002947/4649835‑4650128
                                                                                                                                                   |                                                                                                                                                  
ttGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCtgtg                                                                                                                                                   >  5:4477/1‑149 (MQ=255)
ttGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCtgtg                                                                                                                                                   <  6:4477/149‑1 (MQ=255)
   aCATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCtt                                                                                                                                                <  3:62055/149‑1 (MQ=255)
     aTCGCGCGCGCCGACCCGGTGAATTCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGc                                                                                                                                              >  7:213295/1‑149 (MQ=255)
        tcgcgcccCCACCCGGCGAATCCCCCAGCCCCAGTTCCGCACACGTACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGcc                                                                                                                                             <  2:17398/146‑1 (MQ=255)
           cgcgcCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGcc                                                                                                                                        <  7:275026/149‑1 (MQ=255)
                  aCCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCa                                                                                                                                  >  6:173780/1‑148 (MQ=255)
                  aCCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTAACCGAGGCAGCGATGTAGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGCCTGTGCTTGCCAAGCCCCGGCa                                                                                                                                  >  8:91/1‑148 (MQ=255)
                     cGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCGGGGCTGGGCAAGCACAGCCCTGGcttcga                                                                                                                              <  6:207499/149‑6 (MQ=255)
                      ggTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCGGGGCTGGGCAAGCACAGCCCTGGcttcgag                                                                                                                             <  3:129631/149‑7 (MQ=255)
                            tGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCa                                                                                                                       <  1:133706/149‑1 (MQ=255)
                            tGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCa                                                                                                                       <  2:47867/149‑1 (MQ=255)
                               cccAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCAccc                                                                                                                    >  2:302003/1‑149 (MQ=255)
                               cccAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCAccc                                                                                                                    >  2:173651/1‑149 (MQ=255)
                               cccAGCTCCAGTTCAGCACCCGCACGCCATCATCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGcc                                                                                                                           >  1:89483/1‑142 (MQ=255)
                               cccAGCTCCAGTTCAGCACCCGCACGCCATCATCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGcc                                                                                                                           <  2:89483/142‑1 (MQ=255)
                                 cAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTg                                                                                                                  <  3:159277/149‑1 (MQ=255)
                                   gCTCCAGTGCAGCCCGCGCACTCCAGCTTCGACAAGGTTCACCGAGGGAGGGTGGTTGGCGTTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCGCGGCAAAAAGCCCTCACCCTgcg                                                                                                                <  3:238209/149‑1 (MQ=255)
                                       cAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGc                                                                                                            >  3:9101/1‑149 (MQ=255)
                                       cAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGc                                                                                                            <  3:301752/149‑1 (MQ=255)
                                           tCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCgag                                                                                                         >  3:13191/1‑148 (MQ=255)
                                                  ccGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTg                                                                                                 <  1:173651/149‑1 (MQ=255)
                                                  ccGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTg                                                                                                 >  1:68635/1‑149 (MQ=255)
                                                        gCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCg                                                                                                           >  3:105950/1‑133 (MQ=255)
                                                        gCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCg                                                                                                           <  4:105950/133‑1 (MQ=255)
                                                          cATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATgg                                                                                         <  1:302003/149‑1 (MQ=255)
                                                             cTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTg                                                                                      >  3:69329/1‑149 (MQ=255)
                                                                  aCCAGGTTCTCCGAGGGAGCGATGTTGGCGGTGATACCGGCTTCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCTGCAAAAAGCGCTCACCCTGCGGAGCGAGAATTCCTGCGACATGGCTGCCGTg                                                                                 <  7:171690/149‑1 (MQ=255)
                                                                   ccAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACAATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTgg                                                                                >  2:264242/1‑149 (MQ=255)
                                                                               aGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCt                                                                                                                         <  1:16039/96‑1 (MQ=255)
                                                                               aGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCt                                                                                                                         >  2:16039/1‑96 (MQ=255)
                                                                                  cAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTa                                                                 <  2:68635/149‑1 (MQ=255)
                                                                                                  gATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCCGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTaga                                                 <  7:308622/149‑1 (MQ=255)
                                                                                                       cGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGc                                                                               >  2:291314/1‑114 (MQ=255)
                                                                                                       cGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCAt                                            >  5:160140/1‑149 (MQ=255)
                                                                                                            tCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTg                                       <  1:264242/149‑1 (MQ=255)
                                                                                                                          tACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCa                           <  5:173780/147‑1 (MQ=255)
                                                                                                                            ccATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAg                          <  6:160140/146‑1 (MQ=255)
                                                                                                                            ccATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGcc                        <  4:171013/148‑1 (MQ=255)
                                                                                                                                 aCCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTc                   >  4:131633/1‑148 (MQ=255)
                                                                                                                                 aCCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCg                  <  4:9101/149‑1 (MQ=255)
                                                                                                                                  ccTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGa                 >  3:195323/1‑149 (MQ=255)
                                                                                                                                     cGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGCGCGGAGAGGCAGGTCTGGCCTTGTTCGCAGCCCCCTCGATaa              >  4:201510/1‑149 (MQ=255)
                                                                                                                                      gAAGCAAGGGATGTGCTTGCCCATCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTTCCGGGCGGTTACCGTCGCGAGCGTAGAGGAATGTCTTGCCTTGTTCGCAGCCCCCTCGATAAt             <  7:91/149‑1 (MQ=255)
                                                                                                                                        aGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATgg           <  4:13191/149‑1 (MQ=255)
                                                                                                                                         gCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGc                                              >  8:188988/1‑113 (MQ=255)
                                                                                                                                         gCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGc                                              <  7:188988/113‑1 (MQ=255)
                                                                                                                                          ccAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt         >  1:278336/1‑149 (MQ=255)
                                                                                                                                           cggggCTGGGCAAGCACAGCCGCGGCAATAAGCCCTCACGCTGCGCAGCGAGAATGCCTGCGAGATGGCTGGCGTGGCTTGCCGGGCGGTTACGGTCGCGTGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt         <  8:154078/146‑1 (MQ=255)
                                                                                                                                           cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGc                                              <  3:115850/109‑1 (MQ=255)
                                                                                                                                           cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGc                                              >  4:115850/3‑111 (MQ=255)
                                                                                                                                           cggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt         >  4:89813/3‑148 (MQ=255)
                                                                                                                                           cggggCTGGGCAAGCACAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATCGCTGCCGTGGCATGCCGGGCGGGTACCGGCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCt         >  7:154078/3‑148 (MQ=255)
                                                                                                                                            ggggCTGGGCAAGCACAGCCCTGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGa       <  3:89813/148‑1 (MQ=255)
                                                                                                                                              ggCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAc     >  4:121119/1‑149 (MQ=255)
                                                                                                                                                 tgtgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAcccc  >  7:320851/1‑149 (MQ=255)
                                                                                                                                                   tgCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAAcccc  <  2:278336/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGACATCGCGCGCGCCGACCCGGTGAATGCCCCAGCTCCAGTTCAGCACCCGCACGCCATCTTCGACCAGGTTCACCGAGGCAGCGATGTTGGCGGTGATACCGGCATCCGAGTTGCGCTCTACCATCACCTCGAAGCCAGGGCTGTGCTTGCCCAGCCCCGGCAAAAAGCCCTCACCCTGCGCAGCGAGAATGCCTGCGACATGGCTGCCGTGGCTTGCCGGGCGGTTACCGTCGCGAGCGTAGAGGCATGTCTGGCCTTGTTCGCAGCCCCCTCGATAATGGCTGAACCCC  >  NC_002947/4649835‑4650128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑