Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,658,400 G→T 7.3% A346S (GCG→TCG)  nuoC → NADH‑quinone oxidoreductase subunit C/D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,658,4000GT 7.3% 172.0 / 3.0 55A346S (GCG→TCG) nuoCNADH‑quinone oxidoreductase subunit C/D
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

ACGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGGCCGCCCT  >  NC_002947/4658257‑4658542
                                                                                                                                               |                                                                                                                                              
aCGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATc                                                                                                                                           >  2:225461/1‑149 (MQ=255)
  gTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGCTTCGATCAc                                                                                                                                         <  1:183160/149‑1 (MQ=255)
     aTTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCg                                                                                                                                       <  4:132439/148‑1 (MQ=255)
              atGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCt                                                                                                                             <  7:205406/149‑1 (MQ=255)
                 cTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGCTTCGATCAcg                                                                                                                                        >  8:128151/1‑134 (MQ=255)
                 cTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGCTTCGATCAcg                                                                                                                                        <  7:128151/135‑2 (MQ=255)
                   ggCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCAcc                                                                                                                        >  5:220669/1‑149 (MQ=255)
                        aGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGAGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGcc                                                                                                                   <  2:116714/149‑1 (MQ=255)
                          ttcttcCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCt                                                                                                                  >  5:304337/1‑148 (MQ=255)
                          ttcttcCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCt                                                                                                                  <  6:304337/148‑1 (MQ=255)
                             ttcCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTa                                                                                                              >  3:146037/1‑149 (MQ=255)
                             ttcCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTa                                                                                                              >  3:258742/1‑149 (MQ=255)
                                cGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCg                                                                                                           >  4:322607/1‑149 (MQ=255)
                                        cAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTg                                                                                                   <  1:225461/149‑1 (MQ=255)
                                         aGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTgg                                                                                                  >  3:219287/1‑149 (MQ=255)
                                           ccACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCg                                                                                                >  6:223171/1‑149 (MQ=255)
                                           ccACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCg                                                                                                <  4:219287/149‑1 (MQ=255)
                                                  ctgTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGcc                                                                                           <  4:258742/147‑1 (MQ=255)
                                                  ctgTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCa                                                                                         >  8:147628/1‑149 (MQ=255)
                                                     ttCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGa                                                                                      <  3:322607/149‑1 (MQ=255)
                                                        cTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCt                                                                                   <  4:146037/149‑1 (MQ=255)
                                                        cTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCt                                                                                   >  6:333665/1‑149 (MQ=255)
                                                              aCCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCt                                                                                                                             >  2:158354/1‑101 (MQ=255)
                                                              aCCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCt                                                                                                                             <  1:158354/101‑1 (MQ=255)
                                                              aCCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACg                                                                             >  8:115260/1‑149 (MQ=255)
                                                                cTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGcc                                                                                >  2:71619/1‑144 (MQ=255)
                                                                cTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGcc                                                                                <  1:71619/144‑1 (MQ=255)
                                                                  aCATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGc                                                                         >  7:35387/1‑149 (MQ=255)
                                                                    atacAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACAGACCGCCAGCGAGCCTACACCCTGATCGAAGCGATCACAGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCAGCCACGTGGCTg                                                                       >  7:219891/4‑149 (MQ=255)
                                                                      ccAGGACGTCGGCGCCATGCCCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCCCGACCTGCCACGGGGCTgg                                                                      >  4:97349/1‑148 (MQ=255)
                                                                         ggACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTgg                                                                          <  1:316196/141‑1 (MQ=255)
                                                                         ggACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTgg                                                                          >  2:316196/1‑141 (MQ=255)
                                                                                     cATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCa                                                                               <  7:61244/124‑1 (MQ=255)
                                                                                     cATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCa                                                                               >  8:61244/1‑124 (MQ=255)
                                                                                      aTGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGa                                                     <  2:175358/149‑1 (MQ=255)
                                                                                        gACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCAGGGa                                                                    >  6:119079/1‑132 (MQ=255)
                                                                                        gACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCAGGGa                                                                    <  5:119079/132‑1 (MQ=255)
                                                                                           cccGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcg                                                >  4:149714/1‑149 (MQ=255)
                                                                                             cGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTtcgtc                                              <  7:115260/149‑1 (MQ=255)
                                                                                                    cttcaccttcacCGACCGCCAGCGCGCCTACAACGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATgg                                        >  8:287599/1‑148 (MQ=255)
                                                                                                     ttcaccttcaccGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCt                                      >  7:87233/1‑149 (MQ=255)
                                                                                                        accttcaccGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGc                                    <  5:223171/148‑1 (MQ=255)
                                                                                                                cgaccgCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAgc                              >  6:56519/1‑146 (MQ=255)
                                                                                                                cgaccgCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAgc                              <  5:56519/146‑1 (MQ=255)
                                                                                                                     ggcAGCGCGCCTACTCGGTGATCGAAGCGATCTCCGGCTTCCGGCTGCACGCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTcgac                      <  8:219891/147‑1 (MQ=255)
                                                                                                                      ccAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGc                                    <  6:296917/134‑1 (MQ=255)
                                                                                                                      ccAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGc                                    >  5:296917/1‑134 (MQ=255)
                                                                                                                          cgcgcCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATa                 >  7:53582/1‑149 (MQ=255)
                                                                                                                           gcgcCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATac                <  8:87233/149‑1 (MQ=255)
                                                                                                                             gcCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATacac              <  5:333665/149‑1 (MQ=255)
                                                                                                                               cTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACAcc             >  2:103026/1‑148 (MQ=255)
                                                                                                                                    cggTGATCGCTTCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGgcc       >  8:211206/3‑149 (MQ=255)
                                                                                                                                     cGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGgccg      >  5:23873/1‑149 (MQ=255)
                                                                                                                                     cGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGgccg      <  8:53582/149‑1 (MQ=255)
                                                                                                                                         aTCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGGCCGCCCt  <  4:335102/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
ACGTGATTCGCATCATGCTGGCCGAGTTCTTCCGCATCACCAGCCACCTGCTGTTCCTGGGTACCTACATCCAGGACGTCGGCGCCATGACCCCGGTGTTCTTCACCTTCACCGACCGCCAGCGCGCCTACACCGTGATCGAAGCGATCACCGGCTTCCGCCTGCACCCGGCCTGGTACCGCATCGGTGGCGTCGCCCACGACCTGCCACGTGGCTGGGACAAGCTGGTCAAGGACTTCGTCGAATGGCTGCCCAAGCGCCTCGACGAATACACCAAGGCCGCCCT  >  NC_002947/4658257‑4658542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑