Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,666,312	G→C	8.5%	S26S (TCG→TCC)	nuoL →	NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,666,312	0	G	C	8.5%	180.9 / 7.2	59	S26S (TCG→TCC)	nuoL	NADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base C (3/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

ATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTT  >  NC_002947/4666170‑4666458                                                                                                                                                |                                                                                                                                                   aTCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGACCCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAAc                                                                                                                                              <  2:182253/149‑1 (MQ=255) aTCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCTTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAAc                                                                                                                                              <  3:60041/149‑1 (MQ=255)  tCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATTCTTAAAGAGAGATGCGAGGATGAACCTTCTCTTCCTGACTTTCGTCGT‑TCACCTCCTCGGCATCCTGCTCCTGTCGGTCTCGCGAGGACGGTTATCGGAGAAcc                                                                                                                                             >  8:270336/1‑149 (MQ=255)    ctgctgCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGCACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCGTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTg                                                                                                                                           >  8:185971/1‑149 (MQ=255)       ctgcAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTcc                                                                                                                                        >  4:72434/1‑149 (MQ=255)       ctgcAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGGTCTCGGAGAACCTGTcc                                                                                                                                        >  4:76933/1‑149 (MQ=255)          cAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGcc                                                                                                                                     >  5:288501/1‑149 (MQ=255)          cAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGcc                                                                                                                                     <  5:17547/149‑1 (MQ=255)          cAGCTCTATCCCCGCTTCCACACTCTCTCCCTCCATGCCGCCAGGGAGTTGCTCGGAGGCACATTCTCTTCCTGACATTCGGCTT‑TCCCCTCCCCGGCTTCCTGCAGCTCTCGATCGCGCGCGGACGGTTCTCGGAGAACCTGTCCg                                                                                                                                       <  6:153393/147‑1 (MQ=255)            gCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCt                                                                                                                                   <  5:196248/149‑1 (MQ=255)                     ccgcTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATc                                                                                                                               >  2:151342/1‑144 (MQ=255)                     ccgcTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATc                                                                                                                               <  1:151342/144‑1 (MQ=255)                       gcTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCTGATTAACCTTCTCTTCCTGTAGTTCGGCTTATTCTGTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTc                                                                                                                         <  1:280287/149‑1 (MQ=255)                              cacTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCgg                                                                                                                  >  8:98064/1‑148 (MQ=255)                                ctctCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAg                                                                                                               <  1:314618/149‑1 (MQ=255)                                ctctCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAg                                                                                                               <  8:252769/149‑1 (MQ=255)                                   tcGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAg                                                                                                               >  8:68958/1‑146 (MQ=255)                                   tcGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAg                                                                                                               <  7:68958/146‑1 (MQ=255)                                     gACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGc                                                                                                             <  7:98064/146‑1 (MQ=255)                                           gATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGgcc                                                                                                    <  7:51302/149‑1 (MQ=255)                                                 gcCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACg                                                                                              >  1:255504/1‑149 (MQ=255)                                                  ccAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACgg                                                                                             <  2:255504/149‑1 (MQ=255)                                                   cAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGc                                                                                                                            <  5:351983/117‑1 (MQ=255)                                                   cAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGc                                                                                                                            >  6:351983/1‑117 (MQ=255)                                                    aGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGgcc                                                                                           >  4:156942/1‑149 (MQ=255)                                                    aGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTATCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGgcc                                                                                           >  7:246408/1‑149 (MQ=255)                                                                   tGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTgg                                                                            >  1:317625/1‑149 (MQ=255)                                                                   tGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTgg                                                                            <  3:76933/149‑1 (MQ=255)                                                                   tGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTgg                                                                            <  3:72434/149‑1 (MQ=255)                                                                     aaCCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCa                                                                          <  3:156942/149‑1 (MQ=255)                                                                           ctctTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTTCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGTCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCa                                                                     <  7:270336/148‑1 (MQ=255)                                                                              ttCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAAc                                                                   <  2:317625/147‑1 (MQ=255)                                                                              ttCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGt                                                                 >  1:298744/1‑149 (MQ=255)                                                                              ttCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGGCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAAc                                                                   <  8:307180/147‑1 (MQ=255)                                                                                 cTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGc                                                              >  7:160868/1‑149 (MQ=255)                                                                                             tt‑tCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGcccc                                                           <  3:1279/140‑1 (MQ=255)                                                                                             tt‑tCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGcccc                                                           >  4:1279/1‑140 (MQ=255)                                                                                                   ccTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGggcggc                                              <  6:125262/148‑1 (MQ=255)                                                                                                   ccTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGggcggc                                              >  5:125262/1‑148 (MQ=255)                                                                                                   ccTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGgcggcg                                             >  5:107390/1‑149 (MQ=255)                                                                                                     tCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGcgcg                                           >  3:105122/1‑149 (MQ=255)                                                                                                                ctgctgctgTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAgct                                >  7:241211/1‑149 (MQ=255)                                                                                                                   ctgctgTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAgctg                               <  7:185971/147‑1 (MQ=255)                                                                                                                     gctgTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCtgt                           >  7:314760/1‑149 (MQ=255)                                                                                                                      ctgTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCtgtg                          >  5:181279/1‑149 (MQ=255)                                                                                                                      ctgTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCtgt                           <  6:181279/148‑1 (MQ=255)                                                                                                                       tgTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTgg                         >  7:95830/1‑149 (MQ=255)                                                                                                                        gTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTgg                         >  5:72566/1‑148 (MQ=255)                                                                                                                         tCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTgg                         <  8:314760/147‑1 (MQ=255)                                                                                                                           gTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGt                     <  8:160868/149‑1 (MQ=255)                                                                                                                           gTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGt                     >  5:308871/1‑149 (MQ=255)                                                                                                                                 gcgcgGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGt               <  2:298744/149‑1 (MQ=255)                                                                                                                                   cggacaggTTCTCCGAGAACC‑GTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCgg            >  4:284205/7‑149 (MQ=255)                                                                                                                                    gtacaggTTCTCCGAGAACC‑GTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTAcag                                      >  7:284784/6‑122 (MQ=255)                                                                                                                                    gtacaggTTCTCCGAGAACC‑GTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTAcag                                      <  8:284784/117‑1 (MQ=255)                                                                                                                                          gTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACg      >  8:198359/1‑149 (MQ=255)                                                                                                                                           ttCTCCGAGAA‑CTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTAcag                                      <  5:334850/115‑1 (MQ=255)                                                                                                                                           ttCTCCGAGAA‑CTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTAcag                                      >  6:334850/1‑115 (MQ=255)                                                                                                                                              tcGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCtt  >  7:325853/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                   ATCCTGCTGCAGCTGTATCGCCGCTTCCACACTCTCGACATCGATGCTGCCAGTGAGATGCGCGGATGAACCTTCTCTTCCTGACTTTCGTCTT‑TCCCCTCATCGGCTTCCTGCTGCTGTCGTTCTCGCGCGGACGGTTCTCGGAGAACCTGTCCGCCCTGATCGGCGTCGGCTCGGTAGGCCTGTCGGCCGCCACGGCCGCCTACGTCATCTGGCAGTTCAACGTCGCCCCGCCTGAGGGCGGCGCGTACAGCCAGCTGCTGTGGCAGTGGATGTCGGTGGACGGCTT  >  NC_002947/4666170‑4666458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑