Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,717,954	G→T	5.3%	E173D (GAG→GAT)	fabB →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,717,954	0	G	T	5.3%	243.3 / 3.3	75	E173D (GAG→GAT)	fabB	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (34/37);  new base T (2/2);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

AGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCT  >  NC_002947/4717809‑4718101                                                                                                                                                   |                                                                                                                                                    aGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAg                                                                                                                                                     >  6:164297/1‑146 (MQ=255)  gcgcGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGa                                                                                                                                                 <  4:167841/149‑1 (MQ=255)    gcgTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATc                                                                                                                                               <  5:321118/149‑1 (MQ=255)       tCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAg                                                                                                                                            <  2:207064/149‑1 (MQ=255)        cGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTCCCGCA‑CTGGAGCAGATCCAGt                                                                                                                                           >  4:14658/1‑149 (MQ=255)          gCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTgg                                                                                                                                         <  8:139276/149‑1 (MQ=255)           cccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTggg                                                                                                                                        >  8:197722/1‑149 (MQ=255)            ccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTgggg                                                                                                                                       >  7:29835/1‑149 (MQ=255)            ccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTgggg                                                                                                                                       >  7:146426/1‑149 (MQ=255)             cATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGATCTGCTCCAGTgcggt                                                                                                                                      <  4:256476/149‑2 (MQ=255)                 ccGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAg                                                                                                                                   >  4:198108/1‑148 (MQ=255)                  cgcgTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAg                                                                                                                                   >  7:70183/1‑147 (MQ=255)                   gcgTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTGGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCGAGTGGGGCAAGCAg                                                                                                                                >  4:188239/1‑149 (MQ=255)                         cccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATc                                                                                                                          <  3:198108/149‑1 (MQ=255)                          ccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCg                                                                                                                         >  6:120593/1‑149 (MQ=255)                          ccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCg                                                                                                                         >  6:15448/1‑149 (MQ=255)                                  tGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGcc                                                                                                                 <  6:181298/149‑1 (MQ=255)                                    ggCAGCACCGTGTCGGCATGCCTGGCCACCCCGGTCCAGCTCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCg                                                                                                                <  6:326263/148‑1 (MQ=255)                                          aCCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGt                                                                                                                                           <  6:197897/115‑1 (MQ=255)                                           ccGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGt                                                                                                        <  5:15448/149‑1 (MQ=255)                                            cGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTg                                                                                                       <  8:43245/149‑1 (MQ=255)                                             gtgtCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTg                                                                                                       >  6:257612/1‑148 (MQ=255)                                                   gCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGa                                                                                                                              >  1:143994/1‑119 (MQ=255)                                                   gCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGa                                                                                                                              <  2:143994/119‑1 (MQ=255)                                                          tGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATAGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTg                                                                                          >  6:336262/1‑148 (MQ=255)                                                          tGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCAATGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACggg                                                                                         >  6:68525/1‑149 (MQ=255)                                                            gCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGa                                                                                        <  8:42766/148‑1 (MQ=255)                                                             ccACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGc                                                                                      <  5:48367/149‑1 (MQ=255)                                                             ccACCCCGGTCAAGATCACGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGc                                                                                      <  2:145983/149‑1 (MQ=255)                                                               aCCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTTCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCa                                                                                    <  6:174907/149‑1 (MQ=255)                                                                    gTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGt                                                                               <  3:14658/149‑1 (MQ=255)                                                                       cAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                             <  1:207841/148‑1 (MQ=255)                                                                       cAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                             <  5:257612/148‑1 (MQ=255)                                                                        aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTcc                                                                            <  6:169857/148‑1 (MQ=255)                                                                        aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCt                                                                           <  8:70183/149‑1 (MQ=255)                                                                          gATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGt                                                                         <  3:188239/149‑1 (MQ=255)                                                                                   cATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCa                                                                >  8:89461/1‑149 (MQ=255)                                                                                    aTCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAt                                                               >  6:47838/1‑149 (MQ=255)                                                                                           actCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGcc                                                        >  1:158622/1‑149 (MQ=255)                                                                                            ctCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGCGCCAGTCGTTCCTGTTCGATGCCATGGGCGccc                                                       >  7:196739/1‑149 (MQ=255)                                                                                             tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAg                                                                                                                                >  5:103029/1‑75 (MQ=255)                                                                                             tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAg                                                                                                                                <  6:103029/75‑1 (MQ=255)                                                                                             tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCt                                                      >  2:64795/1‑149 (MQ=255)                                                                                               gATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGt                                                    >  2:98397/1‑149 (MQ=255)                                                                                                   tcgtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTgaagaa                                                                                                  >  2:319146/1‑99 (MQ=255)                                                                                                   tcgtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTgaagaa                                                                                                  <  1:319146/99‑1 (MQ=255)                                                                                                    cgtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGAc                                                >  6:232751/1‑148 (MQ=255)                                                                                                     gtcgtcCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                 <  8:146426/146‑1 (MQ=255)                                                                                                     gtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGt                                                    <  7:197722/143‑1 (MQ=255)                                                                                                     gtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTc                                                   <  7:49104/144‑1 (MQ=255)                                                                                                      tcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCaa                                             <  5:120593/149‑1 (MQ=255)                                                                                                         gCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCTGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAgcg                                          >  8:68992/1‑149 (MQ=255)                                                                                                          ccTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAgcg                                          >  3:44953/1‑148 (MQ=255)                                                                                                              cgcCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAAc                                      <  5:47838/148‑1 (MQ=255)                                                                                                                 cACCTCGGCTCACTGCATCGGTACCGCAGCTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGa                                    >  1:21179/1‑148 (MQ=255)                                                                                                                 cACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGaaa                                  >  1:82178/1‑149 (MQ=255)                                                                                                                  aCCTCGGCTCACTGCATCTGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAc                                 >  4:317188/1‑149 (MQ=255)                                                                                                                   ccTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAcc                                >  7:200712/1‑149 (MQ=255)                                                                                                                       ggCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAcccc                              <  2:158622/147‑1 (MQ=255)                                                                                                                        gCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCgg                            >  4:315829/1‑148 (MQ=255)                                                                                                                         cTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGaa                          <  5:164297/149‑1 (MQ=255)                                                                                                                                cATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTTCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCt                   <  1:98397/149‑1 (MQ=255)                                                                                                                                cATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACt                        <  8:222196/144‑1 (MQ=255)                                                                                                                                cATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACt                        >  7:222196/1‑144 (MQ=255)                                                                                                                                cATCGGGACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGc                     <  8:269841/147‑1 (MQ=255)                                                                                                                                   cGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                >  1:8124/1‑149 (MQ=255)                                                                                                                                    ggTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                <  3:317188/148‑1 (MQ=255)                                                                                                                                     gTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgcgc              >  6:49052/1‑149 (MQ=255)                                                                                                                                        ccGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGcc           >  7:220466/1‑149 (MQ=255)                                                                                                                                        ccGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGcc           <  2:8124/149‑1 (MQ=255)                                                                                                                                         cccA‑CTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                >  2:306529/3‑143 (MQ=255)                                                                                                                                         cccA‑CTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                <  1:306529/141‑1 (MQ=255)                                                                                                                                          ccA‑CTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTa         >  1:140078/2‑149 (MQ=255)                                                                                                                                            a‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                 <  3:132256/107‑1 (MQ=255)                                                                                                                                            a‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                 >  4:132256/1‑107 (MQ=255)                                                                                                                                                  aGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCTGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCt  <  7:68992/149‑1 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGc   <  8:297168/147‑1 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGc   >  7:297168/1‑147 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCt  >  5:245056/1‑148 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCt  >  5:143053/1‑148 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGACCTCGCGCGCCTACGACGCt  >  5:347488/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCT  >  NC_002947/4717809‑4718101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑