Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,717,958 A→C 5.0% I175L (ATC→CTC)  fabB → 3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,717,9580AC 5.0% 244.9 / 4.0 80I175L (ATC→CTC) fabB3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base A (38/38);  new base C (2/2);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

GCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCG  >  NC_002947/4717810‑4718106
                                                                                                                                                     |                                                                                                                                                    
gcgcGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGa                                                                                                                                                      <  4:167841/149‑1 (MQ=255)
  gcgTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATc                                                                                                                                                    <  5:321118/149‑1 (MQ=255)
     tCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAg                                                                                                                                                 <  2:207064/149‑1 (MQ=255)
      cGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTCCCGCA‑CTGGAGCAGATCCAGt                                                                                                                                                >  4:14658/1‑149 (MQ=255)
        gCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTgg                                                                                                                                              <  8:139276/149‑1 (MQ=255)
         cccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTggg                                                                                                                                             >  8:197722/1‑149 (MQ=255)
          ccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTgggg                                                                                                                                            >  7:29835/1‑149 (MQ=255)
          ccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTgggg                                                                                                                                            >  7:146426/1‑149 (MQ=255)
           cATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGATCTGCTCCAGTgcggt                                                                                                                                           <  4:256476/149‑2 (MQ=255)
               ccGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAg                                                                                                                                        >  4:198108/1‑148 (MQ=255)
                cgcgTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAg                                                                                                                                        >  7:70183/1‑147 (MQ=255)
                 gcgTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTGGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCGAGTGGGGCAAGCAg                                                                                                                                     >  4:188239/1‑149 (MQ=255)
                       cccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATc                                                                                                                               <  3:198108/149‑1 (MQ=255)
                        ccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCg                                                                                                                              >  6:15448/1‑149 (MQ=255)
                        ccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCg                                                                                                                              >  6:120593/1‑149 (MQ=255)
                                tGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGcc                                                                                                                      <  6:181298/149‑1 (MQ=255)
                                  ggCAGCACCGTGTCGGCATGCCTGGCCACCCCGGTCCAGCTCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCg                                                                                                                     <  6:326263/148‑1 (MQ=255)
                                        aCCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGt                                                                                                                                                <  6:197897/115‑1 (MQ=255)
                                         ccGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGt                                                                                                             <  5:15448/149‑1 (MQ=255)
                                          cGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTg                                                                                                            <  8:43245/149‑1 (MQ=255)
                                           gtgtCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTg                                                                                                            >  6:257612/1‑148 (MQ=255)
                                                 gCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGa                                                                                                                                   >  1:143994/1‑119 (MQ=255)
                                                 gCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGa                                                                                                                                   <  2:143994/119‑1 (MQ=255)
                                                        tGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATAGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTg                                                                                               >  6:336262/1‑148 (MQ=255)
                                                        tGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCAATGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACggg                                                                                              >  6:68525/1‑149 (MQ=255)
                                                          gCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGa                                                                                             <  8:42766/148‑1 (MQ=255)
                                                           ccACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGc                                                                                           <  5:48367/149‑1 (MQ=255)
                                                           ccACCCCGGTCAAGATCACGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGc                                                                                           <  2:145983/149‑1 (MQ=255)
                                                             aCCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTTCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCa                                                                                         <  6:174907/149‑1 (MQ=255)
                                                                  gTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGt                                                                                    <  3:14658/149‑1 (MQ=255)
                                                                     cAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                                  <  1:207841/148‑1 (MQ=255)
                                                                     cAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                                  <  5:257612/148‑1 (MQ=255)
                                                                      aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTcc                                                                                 <  6:169857/148‑1 (MQ=255)
                                                                      aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCt                                                                                <  8:70183/149‑1 (MQ=255)
                                                                        gATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGt                                                                              <  3:188239/149‑1 (MQ=255)
                                                                                 cATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCa                                                                     >  8:89461/1‑149 (MQ=255)
                                                                                  aTCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAt                                                                    >  6:47838/1‑149 (MQ=255)
                                                                                         actCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGcc                                                             >  1:158622/1‑149 (MQ=255)
                                                                                          ctCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGCGCCAGTCGTTCCTGTTCGATGCCATGGGCGccc                                                            >  7:196739/1‑149 (MQ=255)
                                                                                           tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAg                                                                                                                                     >  5:103029/1‑75 (MQ=255)
                                                                                           tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAg                                                                                                                                     <  6:103029/75‑1 (MQ=255)
                                                                                           tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCt                                                           >  2:64795/1‑149 (MQ=255)
                                                                                             gATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGt                                                         >  2:98397/1‑149 (MQ=255)
                                                                                                 tcgtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTgaagaa                                                                                                       <  1:319146/99‑1 (MQ=255)
                                                                                                 tcgtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTgaagaa                                                                                                       >  2:319146/1‑99 (MQ=255)
                                                                                                  cgtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGAc                                                     >  6:232751/1‑148 (MQ=255)
                                                                                                   gtcgtcCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                      <  8:146426/146‑1 (MQ=255)
                                                                                                   gtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGt                                                         <  7:197722/143‑1 (MQ=255)
                                                                                                   gtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTc                                                        <  7:49104/144‑1 (MQ=255)
                                                                                                    tcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCaa                                                  <  5:120593/149‑1 (MQ=255)
                                                                                                       gCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCTGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAgcg                                               >  8:68992/1‑149 (MQ=255)
                                                                                                        ccTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAgcg                                               >  3:44953/1‑148 (MQ=255)
                                                                                                            cgcCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAAc                                           <  5:47838/148‑1 (MQ=255)
                                                                                                               cACCTCGGCTCACTGCATCGGTACCGCAGCTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGa                                         >  1:21179/1‑148 (MQ=255)
                                                                                                               cACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGaaa                                       >  1:82178/1‑149 (MQ=255)
                                                                                                                aCCTCGGCTCACTGCATCTGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAc                                      >  4:317188/1‑149 (MQ=255)
                                                                                                                 ccTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAcc                                     >  7:200712/1‑149 (MQ=255)
                                                                                                                     ggCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAcccc                                   <  2:158622/147‑1 (MQ=255)
                                                                                                                      gCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCgg                                 >  4:315829/1‑148 (MQ=255)
                                                                                                                       cTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGaa                               <  5:164297/149‑1 (MQ=255)
                                                                                                                              cATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTTCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCt                        <  1:98397/149‑1 (MQ=255)
                                                                                                                              cATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACt                             <  8:222196/144‑1 (MQ=255)
                                                                                                                              cATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACt                             >  7:222196/1‑144 (MQ=255)
                                                                                                                              cATCGGGACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGc                          <  8:269841/147‑1 (MQ=255)
                                                                                                                                 cGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                     >  1:8124/1‑149 (MQ=255)
                                                                                                                                  ggTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                     <  3:317188/148‑1 (MQ=255)
                                                                                                                                   gTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgcgc                   >  6:49052/1‑149 (MQ=255)
                                                                                                                                      ccGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGcc                >  7:220466/1‑149 (MQ=255)
                                                                                                                                      ccGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGcc                <  2:8124/149‑1 (MQ=255)
                                                                                                                                       cccA‑CTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                     <  1:306529/141‑1 (MQ=255)
                                                                                                                                       cccA‑CTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTcgc                     >  2:306529/3‑143 (MQ=255)
                                                                                                                                        ccA‑CTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTa              >  1:140078/2‑149 (MQ=255)
                                                                                                                                          a‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                      <  3:132256/107‑1 (MQ=255)
                                                                                                                                          a‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                      >  4:132256/1‑107 (MQ=255)
                                                                                                                                                aGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCTGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCt       <  7:68992/149‑1 (MQ=255)
                                                                                                                                                 gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGc        >  7:297168/1‑147 (MQ=255)
                                                                                                                                                 gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGc        <  8:297168/147‑1 (MQ=255)
                                                                                                                                                 gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCt       >  5:245056/1‑148 (MQ=255)
                                                                                                                                                 gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCt       >  5:143053/1‑148 (MQ=255)
                                                                                                                                                 gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGACCTCGCGCGCCTACGACGCt       >  5:347488/1‑148 (MQ=255)
                                                                                                                                                  cAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGa     <  7:89461/149‑1 (MQ=255)
                                                                                                                                                     aTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCg  >  2:179424/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCA‑CTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCG  >  NC_002947/4717810‑4718106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑