| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,735,320 | C→T | 100% | M105I (ATG→ATA) | sucA ← | 2‑oxoglutarate dehydrogenase subunit E1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,735,320 | 0 | C | T | 100.0% | 194.5 / NA | 58 | M105I (ATG→ATA) | sucA | 2‑oxoglutarate dehydrogenase subunit E1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/32); total (26/32) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGA > NC_002947/4735176‑4735460 | ctctTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATAc < 6:207862/149‑1 (MQ=255) cGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGtata < 5:182925/140‑1 (MQ=255) cGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGtata > 6:182925/1‑140 (MQ=255) gATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCt < 2:93482/149‑1 (MQ=255) aaCACGTCGCCGGCACGCAAGGTCGTATCAACATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGa < 4:151455/148‑1 (MQ=255) cAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCATGATGGCCGCGTATACGATAGGCCTGGAt > 3:38387/1‑147 (MQ=255) aGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTAt < 8:100268/131‑1 (MQ=255) aGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTAt > 7:100268/1‑131 (MQ=255) gCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCa < 2:22590/149‑1 (MQ=255) gCACGGAAGGTCGTATCAAGATCGGTATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTTATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAAc < 4:92628/149‑1 (MQ=255) cGGAACGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTc < 3:219777/149‑1 (MQ=255) tCGTCTCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGGGCAGGGCGCTGCCACAACCCGAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACTATAGGCCTGGATCAGTCGCAGAACTTCAACCTGc < 4:50545/149‑1 (MQ=255) tCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGc < 1:68008/149‑1 (MQ=255) tCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGc < 8:68809/149‑1 (MQ=255) aTCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttct > 5:73272/1‑149 (MQ=255) tCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCgta > 8:148750/1‑148 (MQ=255) tCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTGTACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTc < 5:59722/149‑1 (MQ=255) aaGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCAc < 6:27462/149‑1 (MQ=255) aaGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCAc > 5:27462/1‑149 (MQ=255) aaGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCAc > 4:77712/1‑149 (MQ=255) aGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCt > 3:324877/1‑99 (MQ=255) aGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCt < 4:324877/99‑1 (MQ=255) cAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCgggt < 6:179934/149‑3 (MQ=255) tACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCAc < 6:162465/116‑1 (MQ=255) tACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCAc > 5:162465/1‑116 (MQ=255) tACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgc > 2:14522/1‑149 (MQ=255) tACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgc > 4:154304/1‑149 (MQ=255) tttctggcAGCGCCCAGCCACAACACCAACGGGTCGAGCTTGTCAGCCTCATCGCCCAGTATACGATAGGCCTGGAACACTCCCAGAACTTCAACCAGCTACTTCAGCTCTACACTGCACACGCTCCCGGCGGAAACCGGTAGggcccg > 6:74890/7‑149 (MQ=255) cGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgc < 3:321368/147‑1 (MQ=255) cgcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCt < 3:154304/149‑1 (MQ=255) tGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc > 3:167911/1‑149 (MQ=255) gCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAACCCGGTTGGGCGCGGCGCTGGTTCTTTGc > 6:5338/1‑148 (MQ=255) gCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc > 4:46080/1‑148 (MQ=255) ccACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc < 5:128792/147‑1 (MQ=255) ccACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc > 2:290289/1‑147 (MQ=255) ccAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTAc > 6:253546/1‑148 (MQ=255) aaCGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTATTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGaa < 7:148750/149‑1 (MQ=255) aCGGGTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGaaa < 3:46080/149‑1 (MQ=255) gTCGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGt > 7:211495/1‑149 (MQ=255) cGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAATCCGGTTGGGCGCGGCGCTGGTTCTTTGc < 1:194572/130‑1 (MQ=255) cGAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc > 2:194572/1‑130 (MQ=255) gAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGt < 8:247811/122‑1 (MQ=255) gAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGt > 7:247811/1‑122 (MQ=255) gAGCTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTcgc < 3:77712/149‑1 (MQ=255) cTTGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGa < 5:253546/149‑1 (MQ=255) ttGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATg > 2:224455/1‑140 (MQ=255) ttGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATg < 1:224455/140‑1 (MQ=255) tGGCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTAAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCAGGCGGAAACCGGTTGGGCGCGGCGATGGTTCTTTGACAGCAGTACGAAATGGTCGCGGAtt > 8:180381/1‑149 (MQ=255) gCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGt > 8:259737/1‑149 (MQ=255) gCAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGt < 1:14522/149‑1 (MQ=255) cAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTc < 7:190870/149‑1 (MQ=255) cAGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTc < 4:167911/149‑1 (MQ=255) aGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCg < 7:259737/149‑1 (MQ=255) aGCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTACAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTc > 7:131661/1‑148 (MQ=255) gCCTGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGa < 4:38387/149‑1 (MQ=255) tGATGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTg > 6:157366/1‑149 (MQ=255) tGGCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGa < 5:5338/149‑1 (MQ=255) ggCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGtt < 3:26759/90‑1 (MQ=255) ggCCGCGTATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGa > 8:140703/1‑148 (MQ=255) ggCCGCGTATACGATAGGCCTGCATCAGTCGCAGAACTTCAACGTCCTTCTTCTCGAGTTCACTGCTCACGCTCCCCGCGGAAACCCGtt > 4:26759/1‑90 (MQ=255) | CTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGAGTGCGA > NC_002947/4735176‑4735460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |