Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,607 G→T 9.7% G73G (GGC→GGA)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,6070GT 9.7% 123.2 / 3.1 41G73G (GGC→GGA) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base T (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGA  >  NC_002947/4744460‑4744754
                                                                                                                                                   |                                                                                                                                                   
ggCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTg                                                                                                                                                     <  5:49251/148‑1 (MQ=255)
 gCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGc                                                                                                                                                    <  4:18605/148‑1 (MQ=255)
 gCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGc                                                                                                                                                    <  6:289672/148‑1 (MQ=255)
   cTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGa                                                                                                                                                 >  6:6768/1‑149 (MQ=255)
   cTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGa                                                                                                                                                 <  6:155846/149‑1 (MQ=255)
        gCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGtgc                                                                                                                                            <  5:6768/149‑3 (MQ=255)
         cAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGtgcg                                                                                                                                           <  2:87300/149‑4 (MQ=255)
          aaGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACt                                                                                                                                          >  5:179151/1‑149 (MQ=255)
                      aGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTc                                                                                                                              >  1:48691/1‑149 (MQ=255)
                       ggcgctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCg                                                                                                                             >  8:286573/1‑149 (MQ=255)
                         cgctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGgc                                                                                                                           <  2:68674/149‑1 (MQ=255)
                           ctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGgcgc                                                                                                                         <  7:29745/149‑1 (MQ=255)
                               cgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGc                                                                                                                     <  7:286573/149‑1 (MQ=255)
                                gctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGc                                                                                                                     >  5:152430/1‑148 (MQ=255)
                                   ccAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGc                                                                                                                 >  1:296319/1‑149 (MQ=255)
                                                 gCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccgcccg                                                                                                   <  4:121513/149‑1 (MQ=255)
                                                     ggCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtc                                                                                               <  6:179151/149‑1 (MQ=255)
                                                            cTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtcac                                                                                        >  2:273512/1‑149 (MQ=255)
                                                            cTGGTTCAGCACCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtcac                                                                                        >  5:315319/1‑149 (MQ=255)
                                                                       ccAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTg                                                                             <  2:48691/149‑1 (MQ=255)
                                                                              ggCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgctgca                                                                      >  5:290793/1‑149 (MQ=255)
                                                                               acATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgcagca                                                                      >  7:237346/2‑148 (MQ=255)
                                                                                 aTGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgcagc                                                                       >  1:55200/1‑145 (MQ=255)
                                                                                 aTGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgcagc                                                                       <  2:55200/145‑1 (MQ=255)
                                                                                 aTGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACAt                                                                   >  3:174962/1‑149 (MQ=255)
                                                                                     tCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCgg                                                                >  7:57112/1‑148 (MQ=255)
                                                                                        cgGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAatc                                                            <  8:237346/149‑1 (MQ=255)
                                                                                                         gcgcTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGa                                           <  6:152430/149‑1 (MQ=255)
                                                                                                          cgcTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGaa                                          <  1:273512/149‑1 (MQ=255)
                                                                                                               gctgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCaa                                     >  4:120470/1‑149 (MQ=255)
                                                                                                               gcggAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCaa                                     >  4:6397/4‑149 (MQ=255)
                                                                                                                   aGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGt                                 <  6:315319/149‑1 (MQ=255)
                                                                                                                     gTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTgg                               >  4:177895/1‑149 (MQ=255)
                                                                                                                      tAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGc                              <  2:296319/149‑1 (MQ=255)
                                                                                                                          cATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCa                          >  3:125592/1‑149 (MQ=255)
                                                                                                                                  gttacaTCGGCACCGGTTCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAgcgc                  >  3:272367/4‑149 (MQ=255)
                                                                                                                                   gCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGcgcg                 >  7:77589/1‑149 (MQ=255)
                                                                                                                                          ggAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGTCAGAGACTCACCCGCCCGAATCACGTCACTCGGCGCGCTGCTCATCACATCGGAATCATCTTTGACACCCTGGAAGGCAACGGTGCCCTCATCCATAGAGGCActgc          >  2:85197/1‑149 (MQ=255)
                                                                                                                                          ggAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCGTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgc          >  5:62472/1‑149 (MQ=255)
                                                                                                                                                 gTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGaa                                          <  2:234217/110‑1 (MQ=255)
                                                                                                                                                 gTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGaa                                          >  1:234217/1‑110 (MQ=255)
                                                                                                                                                  tGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGa  <  4:200872/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGA  >  NC_002947/4744460‑4744754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑