Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,771,576 A→G 7.2% L2564P (CTG→CCG)  pvdD ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,771,5760AG 7.2% 157.7 / 2.3 55L2564P (CTG→CCG) pvdDnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (22/29);  new base G (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCTGGAGGCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTCCAGAGGCTGGTTCTC  >  NC_002947/4771429‑4771721
                                                                                                                                                   |                                                                                                                                                   
gcTGGAGGCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAg                                                                                                                                                    >  1:72620/1‑149 (MQ=255)
 cTGGAGGCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAgg                                                                                                                                                   <  7:19853/149‑1 (MQ=255)
  gggACGCGTCAGCGCCCGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGt                                                                                                                                                  <  8:247608/148‑1 (MQ=255)
    gAGGCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGTGCAGGTa                                                                                                                                                 <  7:251388/148‑1 (MQ=255)
      ggCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACAt                                                                                                                                              <  2:197507/149‑1 (MQ=255)
       gCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATg                                                                                                                                             <  2:52386/149‑1 (MQ=255)
         gTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTa                                                                                                                                           >  3:244050/1‑149 (MQ=255)
          tCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTAc                                                                                                                                          <  1:162318/149‑1 (MQ=255)
              cgcacgcaGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCga                                                                                                                                      >  4:320837/1‑149 (MQ=255)
               gcacgcaGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCgat                                                                                                                                     >  2:311406/1‑149 (MQ=255)
                   gcaGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCCTGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCgatgat                                                                                                                                  >  7:219067/1‑148 (MQ=255)
                      ggggCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAAc                                                                                                                              <  7:138019/149‑1 (MQ=255)
                       gggCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACt                                                                                                                             <  3:317394/149‑1 (MQ=255)
                           gcgcCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTc                                                                                                                         >  8:230564/1‑149 (MQ=255)
                                gggCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTcga                                                                                                                    <  6:239539/149‑1 (MQ=255)
                                 ggCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTcgac                                                                                                                   >  1:78033/1‑149 (MQ=255)
                                   cagcagCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTcgacga                                                                                                                 <  4:24239/149‑1 (MQ=255)
                                    agcagcCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGAt                                                                                                                >  3:214529/1‑149 (MQ=255)
                                        gcCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAAc                                                                                                                              <  6:316021/131‑1 (MQ=255)
                                        gcCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAAc                                                                                                                              >  5:316021/1‑131 (MQ=255)
                                        gcCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGAcc                                                                                                            <  2:72620/149‑1 (MQ=255)
                                          ccGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCAt                                                                                                          >  4:83890/1‑149 (MQ=255)
                                           cGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGCCATCGTCGAAGACCagt                                                                                                         <  7:117402/149‑3 (MQ=255)
                                                    ccAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATc                                                                                                >  5:194864/1‑149 (MQ=255)
                                                     cAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATc                                                                                                >  8:261705/1‑148 (MQ=255)
                                                     cAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATc                                                                                                <  7:261705/148‑1 (MQ=255)
                                                           cgcCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGt                                                                                       <  7:230564/149‑1 (MQ=255)
                                                                ggCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATc                                                                                                         <  7:115848/128‑1 (MQ=255)
                                                                ggCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATc                                                                                                         >  8:115848/1‑128 (MQ=255)
                                                                 gCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGt                                                                                   <  3:320837/147‑1 (MQ=255)
                                                                       cAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCgg                                                                           <  8:219067/149‑1 (MQ=255)
                                                                       cAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCg                                                                            >  8:158266/1‑148 (MQ=255)
                                                                        aGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGa                                                                          <  4:214529/149‑1 (MQ=255)
                                                                        aGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGa                                                                          <  2:78033/149‑1 (MQ=255)
                                                                          cGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGc                                                                        <  4:244050/149‑1 (MQ=255)
                                                                              aGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCAcc                                                                    >  4:16134/1‑149 (MQ=255)
                                                                                      cagcagCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGc                                                            <  3:16134/149‑1 (MQ=255)
                                                                                           gcgcCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAAt                                                       >  2:313593/1‑149 (MQ=255)
                                                                                                                        aCCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAg                          >  8:5365/1‑149 (MQ=255)
                                                                                                                         ccGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAgg                         <  7:158266/149‑1 (MQ=255)
                                                                                                                         ccGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAgg                         <  1:313593/149‑1 (MQ=255)
                                                                                                                          cGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAggg                        >  3:337374/1‑149 (MQ=255)
                                                                                                                                 tCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAgg                                                 <  7:16152/117‑1 (MQ=255)
                                                                                                                                 tCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATAGCCGAATTGCAgg                                                 >  8:16152/1‑117 (MQ=255)
                                                                                                                                   gCTGAAGCACTGGCGCGGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTc                 <  4:61824/147‑1 (MQ=255)
                                                                                                                                   gCTGAAGCACTGGCGCGGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTc                 <  4:57207/147‑1 (MQ=255)
                                                                                                                                   gCTGAAGCACTGGCGCGGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTc                 >  3:61824/1‑147 (MQ=255)
                                                                                                                                   gCTGAAGCACTGGCGCGGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTc                 >  3:57207/1‑147 (MQ=255)
                                                                                                                                          cACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTCCAGAGGCTg        <  3:83890/149‑1 (MQ=255)
                                                                                                                                           aCTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTCCAGAGGCTgg       <  7:5365/149‑1 (MQ=255)
                                                                                                                                            cTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTc                 <  7:156747/138‑1 (MQ=255)
                                                                                                                                            cTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTc                 >  8:156747/1‑138 (MQ=255)
                                                                                                                                              ggCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGAAGAGTCGGACAGGCCGGAGCCACCCACATCCCCGAATTGAAGCCTGTGGTCATACCACTCGCCCAGGGTGCGGTCAACAGCCTGGt      >  1:275609/1‑149 (MQ=255)
                                                                                                                                               gcgcAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTCCAGAGGCTGGTtct   >  5:262320/1‑149 (MQ=255)
                                                                                                                                                cgcAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTCCAGAGGCTGGTtctc  <  6:262320/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTGGAGGCGTCAGCGCACGCAGGGGCGCGCCGGGCAGCAGCCCGAGGTTGCCCAGGCGCGCCTGGCCGTCCAGCGACAGCGCCTGCAGCAGCGCCTCCATGTTGGCGCGAATGCCCGCCACCGCTTCCTCGCTGAAGCACTGGCGCAGGTACATGTACTCGATGATCAACTGGTCTTCGACGATGACCATCAGGTCCATCGGGA‑‑AGTCGGTCAGGCCGGAGCCACCGACATCGCCGAATTGCAGGCTGTCGTCCTTCCACTCGCGCAGGGTGCGGTCCAGAGGCTGGTTCTC  >  NC_002947/4771429‑4771721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑