Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,779,863 T→C 7.8% Y2484C (TAC→TGC)  pvdJ ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,779,8630TC 7.8% 145.1 / 2.1 51Y2484C (TAC→TGC) pvdJnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (22/25);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

ATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGCCACCTGCGCATCCACCACCACGGCGCTGTGCACAC  >  NC_002947/4779720‑4780007
                                                                                                                                               |                                                                                                                                                
aTTGCTGCTGCAACGACGGTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGccc                                                                                                                                             <  6:353551/149‑1 (MQ=255)
    ctgctgcAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCgg                                                                                                                                           >  2:307872/1‑147 (MQ=255)
     tgctgcAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGCGCCTCACCCACAGTGCCGGCACAATGTAGCCCGGAAg                                                                                                                                        >  6:239208/1‑149 (MQ=255)
        tgcAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCAGAATGCt                                                                                                                                     >  4:5374/1‑149 (MQ=255)
          cAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCGTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTgg                                                                                                                                   <  5:266707/149‑1 (MQ=255)
           aaCGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGt                                                                                                                                                  <  1:300398/133‑1 (MQ=255)
           aaCGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGt                                                                                                                                                  >  2:300398/1‑133 (MQ=255)
           aaCGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCgg                                                                                                                                           <  6:332921/140‑1 (MQ=255)
           aaCGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCgg                                                                                                                                           >  5:332921/1‑140 (MQ=255)
            acgacgTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGCAGCCCGGAAGGCTGGCa                                                                                                                                 >  7:98765/1‑149 (MQ=255)
            acgacgTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGCAGCCAGGAAGGCTGGCa                                                                                                                                 >  7:103677/1‑149 (MQ=255)
             cgacgTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGc                                                                                                                                  <  4:312687/147‑1 (MQ=255)
                  tttCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCGACAGTGCCGGCACCATGTAGCCCGGAAGGCTg                                                                                                                                    >  5:297772/1‑140 (MQ=255)
                  tttCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTg                                                                                                                                    <  6:297772/140‑1 (MQ=255)
                  tttCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTg                                                                                                                                    >  7:114059/1‑140 (MQ=255)
                  tttCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTg                                                                                                                                    <  8:114059/140‑1 (MQ=255)
                      cGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGt                                                                                                                       <  4:290623/149‑1 (MQ=255)
                        gTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCg                                                                                                                     <  5:354244/149‑1 (MQ=255)
                        gTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCg                                                                                                                     <  5:32202/149‑1 (MQ=255)
                        gTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCg                                                                                                                     <  6:49596/149‑1 (MQ=255)
                                  gcgcTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGc                                                                                                           <  1:307872/149‑1 (MQ=255)
                                          gAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCAtc                                                                                                   >  1:248889/1‑149 (MQ=255)
                                           aGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCAtct                                                                                                  >  7:1522/1‑149 (MQ=255)
                                                  cTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTgc                                                                                           >  1:301671/1‑149 (MQ=255)
                                                   tCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTgcg                                                                                          >  7:167498/1‑149 (MQ=255)
                                                     gggTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTgcg                                                                                          <  2:140250/147‑1 (MQ=255)
                                                       gTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTgcg                                                                                                       <  4:196050/132‑1 (MQ=255)
                                                       gTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTgcg                                                                                                       >  3:196050/1‑132 (MQ=255)
                                                            gTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAg                                                                                 <  8:1522/149‑1 (MQ=255)
                                                            gTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAg                                                                                 <  2:248889/149‑1 (MQ=255)
                                                                 ggCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATc                                                                            >  7:249807/1‑149 (MQ=255)
                                                                  gCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATcg                                                                           >  5:32748/1‑149 (MQ=255)
                                                                     aTGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGTACGCAGATAGTCGGCCAGAACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCgg                                                                        <  5:4082/149‑1 (MQ=255)
                                                                      tGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCggg                                                                       <  4:331870/149‑1 (MQ=255)
                                                                           tGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGAcc                                                                  >  1:323444/1‑149 (MQ=255)
                                                                            gCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGAcc                                                                  <  2:301671/148‑1 (MQ=255)
                                                                                  gAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAgg                                                           >  8:127254/1‑149 (MQ=255)
                                                                                   aGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGc                                                          >  4:328940/1‑149 (MQ=255)
                                                                                            ttGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTg                                                 >  2:335482/1‑149 (MQ=255)
                                                                                               gCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCtt                                              >  3:185612/1‑149 (MQ=255)
                                                                                               gCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCtt                                              <  1:335482/149‑1 (MQ=255)
                                                                                               gCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCAAGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCt                                               <  5:239208/148‑1 (MQ=255)
                                                                                                   tCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCCGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGCCATCGCGGGAGACCCAGTAGGCCACCAACTGCTTGCCa                                          >  6:328619/1‑149 (MQ=255)
                                                                                                     agaggCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGCAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCAc                                         <  8:98765/145‑1 (MQ=255)
                                                                                                     aGCGGCAGGCTGTCGAGCCTCACCCACATTGCCGGCACCATGCAGCCCGGAAGGCTGGCACGCAGATAGTCTGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCAc                                         <  8:103677/148‑1 (MQ=255)
                                                                                                        ggCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGc                                     <  2:323444/149‑1 (MQ=255)
                                                                                                          cAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGCCa                                   <  1:214390/149‑1 (MQ=255)
                                                                                                                     cccTCACCCACAGTGCCGGCACCATGTAGCCCGGAACGCTGGCACGCAGATAGTCCCCCAGCACGCTGCGCATCTCGGCTGCGCCGACCGAGGCATCGCGGCCGACCCAGTAGGCCACCAACAGCTTGCCCCCGGCCACCTGCGCATc                         >  2:196293/2‑148 (MQ=255)
                                                                                                                        tCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGc                                     >  6:252239/1‑133 (MQ=255)
                                                                                                                        tCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGc                                     <  5:252239/133‑1 (MQ=255)
                                                                                                                                 gTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGCCACCTGCGCATCCACCACCACGgcg            >  7:87410/1‑149 (MQ=255)
                                                                                                                                      tgCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCAAGCTCGCTGCGCATCTCGGCTGCGGCGACCGAGGCATGGCTGGCGAGCCAGTAGGCCACCTACTGCTTGCCAACGGCCAGCTGCTCATCCACCACCACGGCGCtgtg       <  5:328619/148‑1 (MQ=255)
                                                                                                                                           cATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGCCACCTGCGCATCCACCACCACGGCGCTGTGCacac  <  8:249807/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ATTGCTGCTGCAACGACGTTTCCGGTGCCTGGTAGCGCTGCTGAGCGGTGCTCGGGTCCGGTGCCGGCAATGCTTTGCGGTCGAGCTTGCCATTGGCAGTCAGCGGCAGGCTGTCGAGCCTCACCCACAGTGCCGGCACCATGTAGCCCGGAAGGCTGGCACGCAGATAGTCGGCCAGCACGCTGCGCATCTCGGCTGCGGCGACCGAGGCATCGCGGGCGACCCAGTAGGCCACCAACTGCTTGCCACCGGCCACCTGCGCATCCACCACCACGGCGCTGTGCACAC  >  NC_002947/4779720‑4780007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑