Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,858,041 G→C 6.6% G31A (GGG→GCG)  PP_4273 → MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,858,0410GC 6.6% 191.3 / 3.9 61G31A (GGG→GCG) PP_4273MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

TCATTACCATGCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGC  >  NC_002947/4857893‑4858188
                                                                                                                                                    |                                                                                                                                                   
tCATTACCATGCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCgg                                                                                                                                                     <  2:199675/149‑1 (MQ=255)
  aTTACCATGCCGCGCATCATAGAAGTTGAAGTCAAGTTCAAGTCACGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGTCTTGCTCAAGTCCTTGGCCGGgc                                                                                                                                                   <  3:95094/149‑1 (MQ=255)
          gCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGCGCCCGGcc                                                                                                                                             >  7:41451/1‑147 (MQ=255)
            cgcgCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGCGCCCGGCCaagg                                                                                                                                         <  6:160972/149‑4 (MQ=255)
            cgcgCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGCGCCCGGCCaagg                                                                                                                                         <  8:41451/149‑4 (MQ=255)
             gcgcAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGt                                                                                                                                        <  7:189199/149‑1 (MQ=255)
                cagcaTAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGa                                                                                                                                     >  5:276509/1‑149 (MQ=255)
                cagcaTAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGa                                                                                                                                     <  6:123942/149‑1 (MQ=255)
                 agcaTAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGAc                                                                                                                                    <  6:231580/149‑1 (MQ=255)
                  gcaTAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACa                                                                                                                                   <  6:92863/149‑1 (MQ=255)
                      aGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGttt                                                                                                                               >  7:2747/1‑149 (MQ=255)
                       gAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTg                                                                                                                              >  4:109954/1‑149 (MQ=255)
                        aaGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTg                                                                                                                              >  3:71272/1‑148 (MQ=255)
                         aGTTGAAGTCCACTTCAAGTCAATGGCCCGAAATTGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGATAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCa                                                                                                                            <  4:275308/149‑1 (MQ=255)
                               aGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGAt                                                                                                                      <  8:2747/149‑1 (MQ=255)
                                 tCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTc                                                                                                                                       >  1:232497/1‑130 (MQ=255)
                                 tCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTc                                                                                                                                       <  2:232497/130‑1 (MQ=255)
                                   aaCTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTgg                                                                                                                  <  8:8983/149‑1 (MQ=255)
                                           gtcaagGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGcc                                                                                                                         >  1:168979/1‑134 (MQ=255)
                                           gtcaagGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGcc                                                                                                                         <  2:168979/134‑1 (MQ=255)
                                                 ggCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGgcg                                                                                                    >  5:225378/1‑149 (MQ=255)
                                                     cGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGa                                                                                                >  8:4284/1‑149 (MQ=255)
                                                                    cGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAgg                                                                                 <  5:333931/149‑1 (MQ=255)
                                                                    cGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAgg                                                                                 >  1:208990/1‑149 (MQ=255)
                                                                              aaaCGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGtt                                                                       <  4:71272/149‑1 (MQ=255)
                                                                                    aCTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCt                                                                 <  6:276509/149‑1 (MQ=255)
                                                                                      tGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTg                                                                >  2:180543/1‑148 (MQ=255)
                                                                                          gTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATg                                                            >  6:63630/1‑148 (MQ=255)
                                                                                              cTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTgg                                                                                       <  7:297907/117‑1 (MQ=255)
                                                                                              cTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTgg                                                                                       >  8:297907/1‑117 (MQ=255)
                                                                                               tGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgg                                                      >  1:41168/1‑149 (MQ=255)
                                                                                               tGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgg                                                      >  1:319960/1‑149 (MQ=255)
                                                                                                gCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTg                                                       <  7:97730/147‑1 (MQ=255)
                                                                                                gCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTg                                                       >  8:97730/1‑147 (MQ=255)
                                                                                                 cATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgggg                                                    <  2:319960/149‑1 (MQ=255)
                                                                                                        acTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTggggg                                                   <  8:222315/143‑1 (MQ=255)
                                                                                                        acTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTggggg                                                   >  7:222315/1‑143 (MQ=255)
                                                                                                           gCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCtgg                                          >  7:167741/1‑149 (MQ=255)
                                                                                                              ttACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGCTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCcggtcg                                       >  8:109047/1‑147 (MQ=255)
                                                                                                               tactacGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGc                                                                                                                 >  1:15685/1‑74 (MQ=255)
                                                                                                               tactacGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGc                                                                                                                 <  2:15685/74‑1 (MQ=255)
                                                                                                                 ctacGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGAc                                     <  7:4284/148‑1 (MQ=255)
                                                                                                                  tacCAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGCCCAGCGTCGACAGTATGCAGACGATGTGGCAGACCGGTTCCCGCTGATCCCCCTGGGGCTGGCTGCGCGGGAAACGCTGGATGAACTGGGGGCGATGCTGGTGGACCt                                   >  4:170620/1‑149 (MQ=255)
                                                                                                                     gagaAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTg                                  >  8:181816/1‑147 (MQ=255)
                                                                                                                         agaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTc                            <  1:180543/149‑1 (MQ=255)
                                                                                                                          gaaAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCg                           <  2:208990/149‑1 (MQ=255)
                                                                                                                           aaaGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTACGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGa                          >  6:248848/1‑149 (MQ=255)
                                                                                                                              ggCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTGCAGGTCCAcca                       <  3:109954/149‑2 (MQ=255)
                                                                                                                                 ttGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgggg                                                    <  7:89478/117‑1 (MQ=255)
                                                                                                                                 ttGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTgggg                                                    >  8:89478/1‑117 (MQ=255)
                                                                                                                                    cTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGCTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCCGGTCGACCTGCAGGTCCACCAGCatc                  <  7:109047/148‑3 (MQ=255)
                                                                                                                                     tCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGTTCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATg                >  6:105396/1‑149 (MQ=255)
                                                                                                                                     tCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGAcc                                    <  2:37138/129‑1 (MQ=255)
                                                                                                                                     tCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGAcc                                    >  1:37138/1‑129 (MQ=255)
                                                                                                                                      cAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGc               >  6:249010/1‑149 (MQ=255)
                                                                                                                                         gacgctGGCCGCGCCCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTATCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGa            >  5:337193/6‑149 (MQ=255)
                                                                                                                                          tCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGAt           >  3:2451/1‑149 (MQ=255)
                                                                                                                                            cTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATgg         >  5:247980/1‑149 (MQ=255)
                                                                                                                                             ttGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTggggg                                                   >  7:237173/1‑106 (MQ=255)
                                                                                                                                             ttGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTggggg                                                   <  8:237173/106‑1 (MQ=255)
                                                                                                                                               ggCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGTTCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGcc       <  5:105396/148‑1 (MQ=255)
                                                                                                                                               ggCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGc        <  7:181816/147‑1 (MQ=255)
                                                                                                                                                   gggCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTACGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGc  <  5:248848/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCATTACCATGCCGCGCAGCATAGAAGTTGAAGTCAACTTCAAGTCAAGGGCCCGAAATGGACATTGCCGACGTCGCCAAACGCACTGGCGTACCTGCATCGACACTGCGTTACTACGAGAAGAAAGGCTTGCTCAAGTCCTTGGCCGGGCGCGGCCAGCGTCGACAGTTTGCAGCCGATGTGGCAGACCGGTTGGCGCTGATCGCCCTGGGGCAGGCTGCGGGGTTTTCGCTGGATGAAGTGGGGGCGATGCTGGTGGACCTGCAGGTCGACCGGCAGATGCTGATGGCCAAGGC  >  NC_002947/4857893‑4858188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑