Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 407,762 A→T 5.3% A81A (GCT→GCA)  aceF ← AceF‑S‑acetyldihydrolipoate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947407,7620AT 5.3% 225.3 / 2.6 76A81A (GCT→GCA) aceFAceF‑S‑acetyldihydrolipoate
Reads supporting (aligned to +/- strand):  ref base A (32/40);  new base T (2/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

CTTGCCCGACGAACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTCAGCAGGCTCT  >  NC_002947/407615‑407910
                                                                                                                                                   |                                                                                                                                                     
attGCCCGACGAACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCgcag                                                                                                                                                      >  6:351963/2‑149 (MQ=255)
 ttGCCCGACGAACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCgcag                                                                                                                                                      >  3:115097/1‑148 (MQ=255)
      cgacgaACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAgcgg                                                                                                                                                >  6:62247/1‑149 (MQ=255)
       gacgaACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAgcggc                                                                                                                                               <  7:237073/149‑1 (MQ=255)
          gaACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGcc                                                                                                                                            >  6:123952/1‑149 (MQ=255)
            aCCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCt                                                                                                                                          >  8:161712/1‑149 (MQ=255)
             ccGATGTCCGGCACTTGGATGTCCTGCAGGCTGGCAGCAGGCGGGGCTGCGGCGGGAGGAGCAGGAGCGGCTGCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCATCAGCCGGCGCCTCAGGCGCCGCTGCTGCGGcccctga                                                                                                                                         <  2:174312/149‑4 (MQ=255)
              cGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCg                                                                                                                                        >  7:210157/1‑149 (MQ=255)
                aTGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGc                                                                                                                                      <  6:296879/149‑1 (MQ=255)
                    ccGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTcc                                                                                                                                  <  5:351963/149‑1 (MQ=255)
                    ccGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTc                                                                                                                                   >  3:201158/1‑148 (MQ=255)
                     cGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 <  4:201158/149‑1 (MQ=255)
                     cGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 <  7:54985/149‑1 (MQ=255)
                        cACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGa                                                                                                                              >  5:248117/1‑149 (MQ=255)
                         aCGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGAc                                                                                                                             <  7:139332/149‑1 (MQ=255)
                               aTGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGACGCCTCAGGGGCCGCAGCAGCTGCGCCCTCGGCTTCCAGGACcagcag                                                                                                                       <  5:327750/149‑1 (MQ=255)
                                    cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 <  1:139096/134‑1 (MQ=255)
                                    cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 >  2:139096/1‑134 (MQ=255)
                                    cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgt                                                                                                                  >  5:276278/1‑149 (MQ=255)
                                    cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgt                                                                                                                  >  3:37560/1‑149 (MQ=255)
                                    cTGCACGCTGGCAGCAGCCGGGGCTGCGCCCGGAGCAGCCGGAGCGCCTTCAGCGGCTGCCGCTCGCGCCGCAGCCGGAGCAGCGGCCGCAGCCGGCGCCTCAGGGGCCGCACCAGCGGCGCCCTCCGCATCCAGGACCACCAGTacga                                                                                                                  >  7:225578/1‑148 (MQ=255)
                                       cACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgtc                                                                                                                 <  5:2973/147‑1 (MQ=255)
                                        aCGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGcc                                                                                                              >  6:36806/1‑149 (MQ=255)
                                         ctccggCCGCAGCCCCGGCTGCTGCCAGCG‑TGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGcc                                                                                                              <  2:121392/143‑1 (MQ=38)
                                             ggCCGCAGCCCCGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCcttctt                                                                                                         >  6:234958/1‑149 (MQ=255)
                                              gcagcaGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCcttct                                                                                                          <  7:161712/147‑1 (MQ=255)
                                              gcagcaGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCttt                                                                                                        <  6:276278/149‑1 (MQ=255)
                                               cagcagCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTc                                                                                                       <  5:62247/149‑1 (MQ=255)
                                                agcagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCagcagc                                                                                                                                                  <  2:149521/105‑1 (MQ=255)
                                                agcagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGAAGCAGCCGGCGCCTCAGGGGCCGCagcagc                                                                                                                                                  >  1:149521/1‑105 (MQ=255)
                                                 gcagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAg                                                                                                     >  4:308891/1‑149 (MQ=255)
                                                  cagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAgg                                                                                                    <  1:75275/149‑1 (MQ=255)
                                                     ccGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGCGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑gg                                                                                                >  5:154551/1‑149 (MQ=255)
                                                       ggggCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTc                                                                                              >  8:210926/1‑149 (MQ=255)
                                                         ggCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGc                                                                                            <  5:36806/149‑1 (MQ=255)
                                                            tGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTc                                                                                                       <  3:202973/136‑1 (MQ=255)
                                                                gCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCtt                                                                                     >  7:140943/1‑149 (MQ=255)
                                                                   ggAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGAc                                                                                  <  7:183757/149‑1 (MQ=255)
                                                                    gAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGAcc                                                                                 <  4:224267/149‑1 (MQ=255)
                                                                     agcagcAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GATCGCCCAGCTTGACCt                                                                                <  4:37560/149‑1 (MQ=255)
                                                                      gcagcaGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCtt                                                                               <  6:258932/149‑1 (MQ=255)
                                                                      gcagcaGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCtt                                                                               >  5:258932/1‑149 (MQ=255)
                                                                         gcagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑gg                                                                                                >  6:1400/1‑129 (MQ=255)
                                                                         gcagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑gg                                                                                                <  5:1400/129‑1 (MQ=255)
                                                                          cagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGt                                                                           <  6:248117/149‑1 (MQ=255)
                                                                          cagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCCCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGt                                                                           >  2:101092/1‑149 (MQ=255)
                                                                           agGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGtt                                                                          >  2:194883/1‑149 (MQ=255)
                                                                              aGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCt                                                                       <  4:115097/149‑1 (MQ=255)
                                                                               gCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCtt                                                                      >  8:158433/1‑149 (MQ=255)
                                                                                 ggCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGa                                                                    <  7:158433/149‑1 (MQ=255)
                                                                                  gCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGAt                                                                   <  8:140943/149‑1 (MQ=255)
                                                                                    ttCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgtc                                                                                                                 <  4:45504/102‑1 (MQ=255)
                                                                                    ttCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgtc                                                                                                                 >  3:45504/1‑102 (MQ=255)
                                                                                    ttCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCa                                                                 >  3:23673/1‑149 (MQ=255)
                                                                                       aGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGc                                                              <  3:308891/149‑1 (MQ=255)
                                                                                        gCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAg                                                                                        >  7:274365/1‑122 (MQ=255)
                                                                                        gCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAg                                                                                        <  8:274365/122‑1 (MQ=255)
                                                                                         cGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGACGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCa                                                            <  5:223886/149‑1 (MQ=255)
                                                                                         cGGCTGGCGCGGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCa                                                            <  5:228794/149‑1 (MQ=255)
                                                                                          ggCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAg                                                           >  2:271445/1‑149 (MQ=255)
                                                                                           gctggcgctggcGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCGAGCTTGACCTTCAGTTCGTTGATCACGCCAGc                                                          >  8:181158/1‑149 (MQ=255)
                                                                                           gctggcgctggcGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGc                                                          <  1:101092/149‑1 (MQ=255)
                                                                                                cgctggcgcCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGACCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTgg                                                     >  1:77305/1‑149 (MQ=255)
                                                                                                 gctggcgcCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCtt                                                                      <  3:64575/131‑1 (MQ=255)
                                                                                                 gctggcgcCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCtt                                                                      >  4:64575/1‑131 (MQ=255)
                                                                                                       gccgcAGCCGGAGCAGCGGCAGCAGCGGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCg                                               >  1:9502/1‑148 (MQ=255)
                                                                                                             gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAAttag                                        >  7:227095/1‑147 (MQ=255)
                                                                                                             gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAAt                                           <  8:227095/146‑1 (MQ=255)
                                                                                                             gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTc                                        >  2:44205/1‑149 (MQ=255)
                                                                                                             gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTc                                        <  7:210926/149‑1 (MQ=255)
                                                                                                              ccGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGCAGGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTc                                        <  1:271445/149‑1 (MQ=255)
                                                                                                                    cagcGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAg                                 <  8:210157/149‑1 (MQ=255)
                                                                                                                       cGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGcc                              >  6:89973/1‑149 (MQ=255)
                                                                                                                             cagcCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCg                        <  5:89973/149‑1 (MQ=255)
                                                                                                                                  ggCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTc                   <  6:154551/149‑1 (MQ=255)
                                                                                                                                        gagggcgCCGCTGCTGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGt             >  3:87765/7‑149 (MQ=255)
                                                                                                                                        gagggcgCCGCTGCTGCGGC‑CCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTc                         <  6:13902/130‑1 (MQ=255)
                                                                                                                                        gagggcgCCGCTGCTGCGGC‑CCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTc                         >  5:13902/7‑136 (MQ=255)
                                                                                                                                             ggCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        <  5:249171/149‑1 (MQ=255)
                                                                                                                                             ggCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        <  5:123952/149‑1 (MQ=255)
                                                                                                                                                 gcagcaGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        <  1:299423/145‑1 (MQ=255)
                                                                                                                                                 gcagcaGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        >  2:299423/1‑145 (MQ=255)
                                                                                                                                                  cagcagCGGCGCCCTCGGCTTCCAGGAGCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCtt                            <  8:39503/124‑1 (MQ=255)
                                                                                                                                                  cagcagCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCtt                            >  7:39503/1‑124 (MQ=255)
                                                                                                                                                   agcagcGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTCAGCAGGctct  >  4:242117/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTTGCCCGACGAACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTCAGCAGGCTCT  >  NC_002947/407615‑407910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑