| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,445 | G→A | 8.7% | intergenic (+78/‑130) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,445 | 0 | G | A | 8.7% | 158.1 / 4.5 | 57 | intergenic (+78/‑130) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (23/29); new base A (2/3); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01 | |||||||||||
ACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAACTCTT > NC_002947/4896297‑4896588 | acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCACTTAGCGGGTAAATACCCACCGAAAGGGGCGGACCCTGCGCGGTGACCCCTAGGAGCggg > 7:118759/1‑149 (MQ=255) agaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGttt < 5:125231/149‑1 (MQ=255) aagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTAc < 6:278233/149‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTa < 1:183118/143‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTa > 2:183118/1‑143 (MQ=255) gAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAaga < 8:18725/149‑1 (MQ=255) gCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAgg > 5:104342/1‑149 (MQ=255) ggcgTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCAcag < 6:345117‑M2/149‑4 (MQ=255) gttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCa < 5:267700/149‑1 (MQ=255) ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGaa < 1:236149/146‑1 (MQ=255) cGCTGTGGGAATGGGTTTTTTCCCAAAGCGTGATAGGGCCTGCGCCGGCGTCTTCGCGGGTATTTCCGCTCCTACTGGCGCGGACCTTGCGCGGTCCCCCTTAGGAGCGGGTTTACCCGCTAAGAGGCCGGCACAGGc < 8:145332/138‑1 (MQ=38) cGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCCCGGTCCCCCGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCAcaggc < 7:145332‑M2/138‑6 (MQ=255) cTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGCGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCa < 1:47362/149‑1 (MQ=255) tgGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCaaa < 2:246396/148‑1 (MQ=255) ccAAAGCGTGATAGCGCCTGCGCCGGCCTCTTACCGGGTAAATACCCAACAAAAGGCGCGGACCGGGCGCGGTCCCCCCGAGGAGCCGGTTTACCCCCCAAGCGGCCGGCCCAGGCAGAAGCAAAAACAAACCATTTTTTGTTAtttt > 4:125716/1‑147 (MQ=255) gcCGGCCTCTTCGCGGTAAAATCCGCTCCAACAGGCGCGGACCGTGCGCGGTCCCCCGTAGTAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTta < 5:12405/149‑1 (MQ=255) ctctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGt < 3:274953/127‑1 (MQ=255) ctctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGt > 4:274953/1‑127 (MQ=255) ctctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCg > 6:137232/1‑149 (MQ=255) ctTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCt > 2:245540/1‑149 (MQ=255) ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg > 2:331152/1‑149 (MQ=255) ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTg > 2:220461/1‑149 (MQ=255) ttCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGATATAGTCGCTg > 8:319439/1‑149 (MQ=255) cgcgGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCt < 6:104342/146‑1 (MQ=255) gcgGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGtt < 3:267018/136‑1 (MQ=255) gcgGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGtt > 4:267018/1‑136 (MQ=255) gggTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAAt > 7:161051/1‑149 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCAcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgg < 2:344619‑M2/144‑71 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCAcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgg > 1:344619‑M2/1‑74 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCt > 1:323962/1‑149 (MQ=255) gTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCt < 8:161051/149‑1 (MQ=255) aaaTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttct < 7:118287‑M2/149‑78 (MQ=255) aTCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCAcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttg < 7:213233‑M2/149‑80 (MQ=255) tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttgggaaacaacccattcccacagcgagaac > 2:78013‑M2/1‑69 (MQ=255) tCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttgggaaacaacccattcccacagcgagaac < 1:78013‑M2/116‑48 (MQ=255) gCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtggc > 8:118287‑M2/1‑66 (MQ=255) gCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATACACGCACCAGTTATAGTCGCTGGCAATCTGCCCCaa > 4:41489/1‑148 (MQ=255) gCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtgg < 8:269331‑M2/148‑83 (MQ=255) gCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttg < 8:320195‑M2/85‑20 (MQ=255) gCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGATTTACCCGCGAAGAGGCCGGCGcaggcgctatcacgctttg > 7:320195‑M2/1‑66 (MQ=255) ccTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCATATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAAcccc > 8:243621/1‑149 (MQ=255) tACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCAcaggcgctatcacgctttgggaaacaacccattcccacagcgagaacagaacgccgccccctgctcgtgggtcttcttgtggcaggtc < 3:91277‑M2/149‑89 (MQ=255) cgcgGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGa < 5:345117/149‑1 (MQ=255) cgGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGATATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAAc < 7:319439/149‑1 (MQ=255) ggACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGaa < 2:323962/147‑1 (MQ=255) ccGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAgg < 1:331152/149‑1 (MQ=255) cGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcccc > 4:209785/1‑128 (MQ=255) cGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGcccc < 3:209785/128‑1 (MQ=255) cGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGa > 8:196725/1‑149 (MQ=255) gTGCGCTGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGAc < 3:125716/149‑1 (MQ=255) gcgGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAACGCATTTTTTGTTGTTTAACCCGTATAAGCCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGACCAAGGACGCac > 4:95297/1‑148 (MQ=255) ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGa > 1:137206/1‑148 (MQ=255) ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTAATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGa > 8:174664/1‑148 (MQ=255) cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGaa > 7:24269/1‑148 (MQ=255) ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAg > 7:319039/1‑134 (MQ=255) ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAg < 8:319039/134‑1 (MQ=255) gTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGaaaaaa < 5:137232/149‑1 (MQ=255) aGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAACTCtt < 8:99371/149‑1 (MQ=255) | ACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAACCCGTATAAACCCACCCGTTATAGTCGCTGGCAATCTGCCCCAACACCCTTCAAGAACAAGGACGCAACGTGAAAAAACTCTT > NC_002947/4896297‑4896588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |