Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 407,765 A→T 5.3% A80A (GCT→GCA)  aceF ← AceF‑S‑acetyldihydrolipoate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947407,7650AT 5.3% 233.9 / 2.5 76A80A (GCT→GCA) aceFAceF‑S‑acetyldihydrolipoate
Reads supporting (aligned to +/- strand):  ref base A (32/40);  new base T (2/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

CGACGAACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTCAGCAGGCTCT  >  NC_002947/407621‑407910
                                                                                                                                                |                                                                                                                                                  
cgacgaACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAgcgg                                                                                                                                                >  6:62247/1‑149 (MQ=255)
 gacgaACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAgcggc                                                                                                                                               <  7:237073/149‑1 (MQ=255)
    gaACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGcc                                                                                                                                            >  6:123952/1‑149 (MQ=255)
      aCCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCt                                                                                                                                          >  8:161712/1‑149 (MQ=255)
       ccGATGTCCGGCACTTGGATGTCCTGCAGGCTGGCAGCAGGCGGGGCTGCGGCGGGAGGAGCAGGAGCGGCTGCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCATCAGCCGGCGCCTCAGGCGCCGCTGCTGCGGcccctga                                                                                                                                         <  2:174312/149‑4 (MQ=255)
        cGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCg                                                                                                                                        >  7:210157/1‑149 (MQ=255)
          aTGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGc                                                                                                                                      <  6:296879/149‑1 (MQ=255)
              ccGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTcc                                                                                                                                  <  5:351963/149‑1 (MQ=255)
              ccGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTc                                                                                                                                   >  3:201158/1‑148 (MQ=255)
               cGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 <  4:201158/149‑1 (MQ=255)
               cGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 <  7:54985/149‑1 (MQ=255)
                  cACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGa                                                                                                                              >  5:248117/1‑149 (MQ=255)
                   aCGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGAc                                                                                                                             <  7:139332/149‑1 (MQ=255)
                         aTGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGACGCCTCAGGGGCCGCAGCAGCTGCGCCCTCGGCTTCCAGGACcagcag                                                                                                                       <  5:327750/149‑1 (MQ=255)
                              cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 >  2:139096/1‑134 (MQ=255)
                              cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCa                                                                                                                                 <  1:139096/134‑1 (MQ=255)
                              cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgt                                                                                                                  >  5:276278/1‑149 (MQ=255)
                              cTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgt                                                                                                                  >  3:37560/1‑149 (MQ=255)
                              cTGCACGCTGGCAGCAGCCGGGGCTGCGCCCGGAGCAGCCGGAGCGCCTTCAGCGGCTGCCGCTCGCGCCGCAGCCGGAGCAGCGGCCGCAGCCGGCGCCTCAGGGGCCGCACCAGCGGCGCCCTCCGCATCCAGGACCACCAGTacga                                                                                                                  >  7:225578/1‑148 (MQ=255)
                                 cACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgtc                                                                                                                 <  5:2973/147‑1 (MQ=255)
                                  aCGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGcc                                                                                                              >  6:36806/1‑149 (MQ=255)
                                   ctccggCCGCAGCCCCGGCTGCTGCCAGCG‑TGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGcc                                                                                                              <  2:121392/143‑1 (MQ=38)
                                       ggCCGCAGCCCCGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCcttctt                                                                                                         >  6:234958/1‑149 (MQ=255)
                                        gcagcaGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCcttct                                                                                                          <  7:161712/147‑1 (MQ=255)
                                        gcagcaGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCttt                                                                                                        <  6:276278/149‑1 (MQ=255)
                                         cagcagCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTc                                                                                                       <  5:62247/149‑1 (MQ=255)
                                          agcagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCagcagc                                                                                                                                                  <  2:149521/105‑1 (MQ=255)
                                          agcagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGAAGCAGCCGGCGCCTCAGGGGCCGCagcagc                                                                                                                                                  >  1:149521/1‑105 (MQ=255)
                                           gcagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAg                                                                                                     >  4:308891/1‑149 (MQ=255)
                                            cagcCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAgg                                                                                                    <  1:75275/149‑1 (MQ=255)
                                               ccGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGCGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑gg                                                                                                >  5:154551/1‑149 (MQ=255)
                                                 ggggCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTc                                                                                              >  8:210926/1‑149 (MQ=255)
                                                   ggCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGc                                                                                            <  5:36806/149‑1 (MQ=255)
                                                      tGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTc                                                                                                       <  3:202973/136‑1 (MQ=255)
                                                          gCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCtt                                                                                     >  7:140943/1‑149 (MQ=255)
                                                             ggAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGAc                                                                                  <  7:183757/149‑1 (MQ=255)
                                                              gAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGAcc                                                                                 <  4:224267/149‑1 (MQ=255)
                                                               agcagcAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GATCGCCCAGCTTGACCt                                                                                <  4:37560/149‑1 (MQ=255)
                                                                gcagcaGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCtt                                                                               <  6:258932/149‑1 (MQ=255)
                                                                gcagcaGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCtt                                                                               >  5:258932/1‑149 (MQ=255)
                                                                   gcagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑gg                                                                                                <  5:1400/129‑1 (MQ=255)
                                                                   gcagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑gg                                                                                                >  6:1400/1‑129 (MQ=255)
                                                                    cagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGt                                                                           <  6:248117/149‑1 (MQ=255)
                                                                    cagGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCCCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGt                                                                           >  2:101092/1‑149 (MQ=255)
                                                                     agGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGtt                                                                          >  2:194883/1‑149 (MQ=255)
                                                                        aGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCt                                                                       <  4:115097/149‑1 (MQ=255)
                                                                         gCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCtt                                                                      >  8:158433/1‑149 (MQ=255)
                                                                           ggCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGa                                                                    <  7:158433/149‑1 (MQ=255)
                                                                            gCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGAt                                                                   <  8:140943/149‑1 (MQ=255)
                                                                              ttCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgtc                                                                                                                 >  3:45504/1‑102 (MQ=255)
                                                                              ttCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTtcgtc                                                                                                                 <  4:45504/102‑1 (MQ=255)
                                                                              ttCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCa                                                                 >  3:23673/1‑149 (MQ=255)
                                                                                 aGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGc                                                              <  3:308891/149‑1 (MQ=255)
                                                                                  gCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAg                                                                                        >  7:274365/1‑122 (MQ=255)
                                                                                  gCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAg                                                                                        <  8:274365/122‑1 (MQ=255)
                                                                                   cGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGACGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCa                                                            <  5:223886/149‑1 (MQ=255)
                                                                                   cGGCTGGCGCGGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCa                                                            <  5:228794/149‑1 (MQ=255)
                                                                                    ggCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAg                                                           >  2:271445/1‑149 (MQ=255)
                                                                                     gctggcgctggcGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCGAGCTTGACCTTCAGTTCGTTGATCACGCCAGc                                                          >  8:181158/1‑149 (MQ=255)
                                                                                     gctggcgctggcGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGc                                                          <  1:101092/149‑1 (MQ=255)
                                                                                          cgctggcgcCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGACCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTgg                                                     >  1:77305/1‑149 (MQ=255)
                                                                                           gctggcgcCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCtt                                                                      <  3:64575/131‑1 (MQ=255)
                                                                                           gctggcgcCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCtt                                                                      >  4:64575/1‑131 (MQ=255)
                                                                                                 gccgcAGCCGGAGCAGCGGCAGCAGCGGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCg                                               >  1:9502/1‑148 (MQ=255)
                                                                                                       gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAAttag                                        >  7:227095/1‑147 (MQ=255)
                                                                                                       gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAAt                                           <  8:227095/146‑1 (MQ=255)
                                                                                                       gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTc                                        >  2:44205/1‑149 (MQ=255)
                                                                                                       gCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTc                                        <  7:210926/149‑1 (MQ=255)
                                                                                                        ccGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGCAGGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTc                                        <  1:271445/149‑1 (MQ=255)
                                                                                                              cagcGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAg                                 <  8:210157/149‑1 (MQ=255)
                                                                                                                 cGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGcc                              >  6:89973/1‑149 (MQ=255)
                                                                                                                       cagcCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCg                        <  5:89973/149‑1 (MQ=255)
                                                                                                                            ggCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTc                   <  6:154551/149‑1 (MQ=255)
                                                                                                                                  gagggcgCCGCTGCTGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGt             >  3:87765/7‑149 (MQ=255)
                                                                                                                                  gagggcgCCGCTGCTGCGGC‑CCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTc                         <  6:13902/130‑1 (MQ=255)
                                                                                                                                  gagggcgCCGCTGCTGCGGC‑CCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTc                         >  5:13902/7‑136 (MQ=255)
                                                                                                                                       ggCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        <  5:123952/149‑1 (MQ=255)
                                                                                                                                       ggCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        <  5:249171/149‑1 (MQ=255)
                                                                                                                                           gcagcaGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        <  1:299423/145‑1 (MQ=255)
                                                                                                                                           gcagcaGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTcagca        >  2:299423/1‑145 (MQ=255)
                                                                                                                                            cagcagCGGCGCCCTCGGCTTCCAGGAGCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCtt                            <  8:39503/124‑1 (MQ=255)
                                                                                                                                            cagcagCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCtt                            >  7:39503/1‑124 (MQ=255)
                                                                                                                                             agcagcGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTCAGCAGGctct  >  4:242117/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGACGAACCGATGTCCGGCACGTGGATGTCCTGCACGCTGGCAGCAGCCGGGGCTGCGGCCGGAGCAGCAGGAGCGGCTTCAGCGGCTGGCGCTGGCGCCGCAGCCGGAGCAGCGGCAGCAGCCGGCGCCTCAGGGGCCGCAGCAGCGGCGCCCTCGGCTTCCAGGACCAGCAGTTCGTCGCCTTCTTTCAGGC‑GGTCGCCCAGCTTGACCTTCAGTTCCTTGATCACGCCAGCCTTGGGCGCCGGAATTTCCATGGAGGCCTTGTCGGACTCCAGGGTCAGCAGGCTCT  >  NC_002947/407621‑407910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑