Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,904,892 T→A 7.1% intergenic (‑59/+176) PP_4312 ← / ← PP_4313 hypothetical protein/peptidylprolyl isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,904,8920TA 7.1% 160.3 / 2.6 56intergenic (‑59/+176)PP_4312/PP_4313hypothetical protein/peptidylprolyl isomerase
Reads supporting (aligned to +/- strand):  ref base T (23/29);  new base A (2/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

AGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTG  >  NC_002947/4904744‑4905039
                                                                                                                                                    |                                                                                                                                                   
aGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTCACCCGCCACATCGCCCGGCTAGtt                                                                                                                                                     <  2:40763/149‑1 (MQ=255)
     ccAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAgg                                                                                                                                                >  4:246207/1‑149 (MQ=255)
      cAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAggg                                                                                                                                               >  3:105653/1‑149 (MQ=255)
          gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGcc                                                                                                                                           >  5:189232/1‑149 (MQ=255)
           ccAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGccc                                                                                                                                          <  6:292789/149‑1 (MQ=255)
              aCGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTg                                                                                                                                       >  4:145359/1‑149 (MQ=255)
                     ggcgATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGaa                                                                                                                                <  4:52945/149‑1 (MQ=255)
                      gcgATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAAt                                                                                                                               <  6:189232/149‑1 (MQ=255)
                             cAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTCCCCGCCACATCGCCCTGCAACTAGCCGGgctatg                                                                                                                                          <  5:113105/131‑5 (MQ=255)
                                tGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTg                                                                                                                     <  1:13936/149‑1 (MQ=255)
                                 gAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTg                                                                                                                     <  3:124415/148‑1 (MQ=255)
                                  aGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTgg                                                                                                                    <  3:246207/148‑1 (MQ=255)
                                  aGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGCCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTATTTGCATGGCGCCCGTGCAGTGAATGCCATCCTTGGc                                                                                                                   <  5:200881/149‑1 (MQ=255)
                                   gCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGc                                                                                                                   <  8:116007/148‑1 (MQ=255)
                                    cACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCAAATCGCCCGGCTAGTTGCa                                                                                                                                                  >  6:314064/1‑116 (MQ=255)
                                    cACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCAAATCGCCCGGCTAGTTGCa                                                                                                                                                  <  5:314064/116‑1 (MQ=255)
                                     aCCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAAc                                                                                                                >  8:314494/1‑149 (MQ=255)
                                       cAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCTCATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAAcgc                                                                                                              >  6:348218/1‑149 (MQ=255)
                                           cTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGa                                                                                                          <  7:314494/149‑1 (MQ=255)
                                           cTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGa                                                                                                          <  3:97045/149‑1 (MQ=255)
                                            tCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAg                                                                                                         >  1:209522/1‑149 (MQ=255)
                                                 agcaAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCa                                                                                                    <  2:209522/149‑1 (MQ=255)
                                                    aaGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCagag                                                                                                 <  5:161209/149‑1 (MQ=255)
                                                                   cGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGtt                                                                                  >  2:118127/1‑149 (MQ=255)
                                                                   cGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGtt                                                                                  <  3:145359/149‑1 (MQ=255)
                                                                       aGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATTCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGt                                                                              <  1:67318/149‑1 (MQ=255)
                                                                                  aaCTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGtt                                                                                  >  3:174945/1‑134 (MQ=255)
                                                                                  aaCTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGtt                                                                                  <  4:174945/134‑1 (MQ=255)
                                                                                        atcaAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTgg                                                                               >  3:219812/1‑131 (MQ=255)
                                                                                        atcaAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTgg                                                                               <  4:219812/131‑1 (MQ=255)
                                                                                        atcaAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGaa                                                             >  8:92753/1‑149 (MQ=255)
                                                                                        aaaaaGGCAGGATCGTGTGGCAGAGAAAATCGGGATTACCCAACACATCGCCCGGCTACTTGCAGGGCGCCAGTGCAGTGAATGCCATCCTTGTAAACCCGTGAGGGTCAGAGTGCCCCGAACCCTTTTGGATCCTACCTGGAGGTGaa                                                             >  8:228758/4‑149 (MQ=255)
                                                                                         tcaAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAAt                                                            >  1:134022/1‑149 (MQ=255)
                                                                                           aaGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATg                                                           <  2:134022/148‑1 (MQ=255)
                                                                                                 ggcTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTcc                                                    <  4:235303/149‑1 (MQ=255)
                                                                                                             agagAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGcc                                        <  4:105653/149‑1 (MQ=255)
                                                                                                              gagaATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGACCCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGcc                                        >  2:178461/1‑148 (MQ=255)
                                                                                                                        ggTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAgggg                             >  2:27151/1‑149 (MQ=255)
                                                                                                                         gTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGc                            >  3:85883/1‑149 (MQ=255)
                                                                                                                         gTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGc                            >  8:215577/1‑149 (MQ=255)
                                                                                                                          tttACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCt                           <  1:27151/149‑1 (MQ=255)
                                                                                                                          tttACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCt                           <  7:92753/149‑1 (MQ=255)
                                                                                                                             aCCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTgg                         <  4:85883/148‑1 (MQ=255)
                                                                                                                              cccGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTc                                                     >  6:119490/1‑119 (MQ=255)
                                                                                                                              cccGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTc                                                     <  5:119490/119‑1 (MQ=255)
                                                                                                                                 gcacgggcGCCCTGCAACTAGCCGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGtt                    >  5:348697/8‑149 (MQ=255)
                                                                                                                                 gCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTa                                      >  3:65842/1‑131 (MQ=255)
                                                                                                                                 gCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTa                                      <  4:65842/131‑1 (MQ=255)
                                                                                                                                    acaTCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTgcg                 >  1:328964/1‑149 (MQ=255)
                                                                                                                                     gggcGCCCTGCAACTAGCCGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTgg                         >  2:60580/4‑140 (MQ=255)
                                                                                                                                     gggcGCCCTGCAACTAGCCGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTgg                         <  1:60580/137‑1 (MQ=255)
                                                                                                                                       tCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGCTTAGGGGCTGGGTGTTGCGCaa              <  7:215577/149‑1 (MQ=255)
                                                                                                                                         gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAAtt            >  2:165390/1‑149 (MQ=255)
                                                                                                                                         gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAAtt            <  1:118127/149‑1 (MQ=255)
                                                                                                                                               cTAGTTGCAGGGCGCCCGTGCAGTTAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTaa      <  7:228758/149‑1 (MQ=255)
                                                                                                                                                 aGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAAtt            >  4:214515/1‑141 (MQ=255)
                                                                                                                                                 aGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAAtt            <  3:214515/141‑1 (MQ=255)
                                                                                                                                                   ttGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTg  >  5:115879/1‑149 (MQ=255)
                                                                                                                                                    tGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTg  >  1:62850/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCAGGCTCGTGTGGCAGAGAATATCGGGTTTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTGAGGGTCAGAGTGCCCCGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTG  >  NC_002947/4904744‑4905039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑