Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,961,593 C→T 6.0% R282H (CGC→CAC)  atoC ← two‑component system DNA‑binding transcriptional activator AtoC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,961,5930CT 6.0% 199.8 / 2.0 67R282H (CGC→CAC) atoCtwo‑component system DNA‑binding transcriptional activator AtoC
Reads supporting (aligned to +/- strand):  ref base C (31/31);  new base T (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

CGCTGGCGAGAGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGCCA  >  NC_002947/4961445‑4961737
                                                                                                                                                     |                                                                                                                                                 
cGCTGGCGAGAGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTcgc                                                                                                                                                   <  4:195744/149‑1 (MQ=255)
     gCGAGAGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGaa                                                                                                                                               <  3:39220/148‑1 (MQ=255)
     gCGAGAGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGa                                                                                                                                                <  8:125568/147‑1 (MQ=255)
     gCGAGAGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAg                                                                                                                                              <  4:134655/149‑1 (MQ=255)
       gagaGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGTGGAAgcg                                                                                                                                            >  4:198889/1‑149 (MQ=255)
       gagaGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAgcg                                                                                                                                            <  7:7605/149‑1 (MQ=255)
            gcgcACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAg                                                                                                                                      >  1:46288/1‑149 (MQ=255)
              gcACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGTGGAAGCGC‑CCAgcc                                                                                                                                    <  3:198889/149‑1 (MQ=255)
                  cGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCa                                                                                                                                <  5:108691/149‑1 (MQ=255)
                   gAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCAc                                                                                                                               <  5:285993/149‑1 (MQ=255)
                     ggCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACtt                                                                                                                             <  1:136315/149‑1 (MQ=255)
                           cTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCg                                                                                                                        <  3:207156/148‑1 (MQ=255)
                           cTTCATCTTGGCAACATGGCGCGCCAGCAGGAGCTCGGCCAACTGCAGGATATCGCCCGGCAGCACACGCCGCGGGCGCCAGGCCAGCGGGAACAACGCCAGGCGTTAGTACA‑TGTCTGCGAGGCAGCGC‑CCAGCCGCCACTTCGCCgg                                                                                                                       >  3:32298/1‑149 (MQ=255)
                           cTTCATCTTGGCAACAGGGCGCGCATGCAGGCGCTCGGCAAAGTGCAGTATATCGCCCGGCCGCTCACGCTGCGGGCGCCAGGCCAGCGGCAACACTGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCg                                                                                                                        <  1:86133/148‑1 (MQ=255)
                                tCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAAGTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAgg                                                                                                                  >  7:182247/1‑149 (MQ=255)
                                  ttGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTc                                                                                                                >  5:284161/1‑149 (MQ=255)
                                    ggCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAgg                                                                                                                  <  3:238403/145‑1 (MQ=255)
                                    ggCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGt                                                                                                                 <  7:179055/146‑1 (MQ=255)
                                       cacaTGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGTGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGt                                                                                                           >  4:233724/1‑149 (MQ=255)
                                       cacaTGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGt                                                                                                           >  4:248954/1‑149 (MQ=255)
                                                 gcCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTtggtg                                                                                                      <  4:140611/144‑1 (MQ=255)
                                                 gcCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTtggtg                                                                                                      >  3:140611/1‑144 (MQ=255)
                                                 gcCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGc                                                                                                 <  1:43730/149‑1 (MQ=255)
                                                  ccAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGcc                                                                                                <  6:93332/149‑1 (MQ=255)
                                                   cagcagGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGc                                                                                                 >  1:255056/1‑147 (MQ=255)
                                                   cagcagGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCa                                                                                               <  8:182247/149‑1 (MQ=255)
                                                           gcTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCt                                                                                        >  3:58588/1‑148 (MQ=255)
                                                             tCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACCGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTCGTGGCCAGCACCCTAAt                                                                                     >  6:243578/1‑149 (MQ=255)
                                                                 ccAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCAAGGCCAGAGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTc                                                                                  >  1:47768/1‑148 (MQ=255)
                                                                  cAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCa                                                                                <  4:58588/149‑1 (MQ=255)
                                                                  cAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCa                                                                                <  4:248794/149‑1 (MQ=255)
                                                                    aCTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAgg                                                                              <  8:314378/149‑1 (MQ=255)
                                                                           gATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCg                                                                       >  4:293405/1‑149 (MQ=255)
                                                                           gATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCg                                                                       >  3:300413/1‑149 (MQ=255)
                                                                             tatCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGTGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGc                                                                     <  3:233724/149‑1 (MQ=255)
                                                                             tatCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGc                                                                     >  2:206041/1‑149 (MQ=255)
                                                                               tCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCtt                                                                   <  2:46288/149‑1 (MQ=255)
                                                                                cGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTg                                                                  >  8:187578/1‑149 (MQ=255)
                                                                                 gCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTgc                                                                 >  2:147688/1‑149 (MQ=255)
                                                                                  cccGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTgcg                                                                >  4:179048/1‑149 (MQ=255)
                                                                                         gCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAgg                                                                              >  2:291331/1‑128 (MQ=255)
                                                                                         gCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGcgcccgc                                                           <  8:154904/147‑1 (MQ=255)
                                                                                         gCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGcgcccgc                                                           >  7:154904/1‑147 (MQ=255)
                                                                                              cGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCg                                                    >  2:308675/1‑149 (MQ=255)
                                                                                               gCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACATGGTCTTCCCGCAAGCGC‑CCCGCCGCCCCTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCCCCCTAATGTCCCGGCGGATCGGCTTGCGCCCGCCTCCCCg                                                    >  1:245182/1‑149 (MQ=255)
                                                                                                  gCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCATCTCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGGGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTcc                                                <  8:137325/149‑1 (MQ=255)
                                                                                                           aggccagCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGCGCCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTcgcg                                        >  1:258651/1‑149 (MQ=255)
                                                                                                               cagCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCt                                   >  8:129913/1‑149 (MQ=255)
                                                                                                               cagCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCt                                   >  7:212452/1‑149 (MQ=255)
                                                                                                                agCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTg                                  <  1:206041/149‑1 (MQ=255)
                                                                                                                agCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTg                                  <  3:179048/149‑1 (MQ=255)
                                                                                                                agCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTg                                  >  4:115077/1‑149 (MQ=255)
                                                                                                                 gCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTgc                                 <  3:248954/149‑1 (MQ=255)
                                                                                                                 gCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTgc                                 <  7:187578/149‑1 (MQ=255)
                                                                                                                    ggAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTgcagc                              <  3:255288/149‑1 (MQ=255)
                                                                                                                    ggAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTgcag                               >  6:290952/1‑148 (MQ=255)
                                                                                                                     gAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTgcagca                             >  5:146475/1‑149 (MQ=255)
                                                                                                                           cGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACg                         >  6:155465/1‑147 (MQ=255)
                                                                                                                             aCAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACgcagc                     <  2:255056/149‑1 (MQ=255)
                                                                                                                                   ggTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTg               <  6:146475/149‑1 (MQ=255)
                                                                                                                                     tagtaCA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGc             <  5:155465/149‑1 (MQ=255)
                                                                                                                                          cA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGc         >  2:346526/1‑148 (MQ=255)
                                                                                                                                           a‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCaa       <  3:324574/149‑1 (MQ=255)
                                                                                                                                               tCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGc    <  1:346526/149‑1 (MQ=255)
                                                                                                                                                cTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGc    >  5:321279/1‑148 (MQ=255)
                                                                                                                                                 ttCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGCCa  >  2:334367/1‑149 (MQ=255)
                                                                                                                                                 ttCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGCCa  >  8:29092/1‑149 (MQ=255)
                                                                                                                                                 ttCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGCCa  >  1:108815/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CGCTGGCGAGAGGCGCACCGAGGCATGCTTCATCTTGGCCACATGGCGCGCCAGCAGGCGCTCGGCCAACTGCAGGATATCGCCCGGCCGCTCACGCAGCGGGCGCCAGGCCAGCGGGAACACCGACAGGCGGTAGTACA‑GGTCTTCGCGGAAGCGC‑CCAGCCGCCACTTCGCCGGCCAGGTCGCGGTTGGTGGTGGCCAGCACCCTAATGTCCAGGCTGATCGGCTTGCGCCCGCCTACCCGCTCCACCTCGCGTTCCTGCAGCACACGCAGCAGCTTGGCCTGCAAGGCCA  >  NC_002947/4961445‑4961737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑