Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,028,905 G→A 6.8% K391K (AAG→AAA)  PP_4432 → Xaa‑Pro aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,028,9050GA 6.8% 260.5 / 6.2 88K391K (AAG→AAA) PP_4432Xaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand):  ref base G (41/41);  new base A (3/3);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

GCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAG  >  NC_002947/5028760‑5029051
                                                                                                                                                 |                                                                                                                                                  
gcCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCtt                                                                                                                                                 >  5:87315/1‑149 (MQ=255)
 cccGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCttt                                                                                                                                                >  3:286910/1‑149 (MQ=255)
   cGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTTGCAAACTTTTc                                                                                                                                              <  5:7152/149‑1 (MQ=255)
   cGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGGCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTc                                                                                                                                              <  2:148377/149‑1 (MQ=255)
    gAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCg                                                                                                                                             >  6:73949/1‑149 (MQ=255)
       cGACAACAGAGTTCTCAATCATTGTATGACCTTCCACTTCATGCCTGTCATATTGATGGATGATTGGGGGCTCGATATTACCGAGAGCATGCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTc                                                                                                                                           <  7:287082/148‑1 (MQ=255)
        gACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                                                                                                                                         <  2:79286/149‑1 (MQ=255)
         acaacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAg                                                                                                                                        >  6:94455/1‑149 (MQ=255)
           aacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAgg                                                                                                                                       >  4:324048/1‑148 (MQ=255)
           aacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAACTTTTCGTCagtcc                                                                                                                                      >  8:98494/1‑145 (MQ=255)
           aacaGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAACTTTTCGTCagtcc                                                                                                                                      >  8:93716/1‑145 (MQ=255)
               gCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGCCGTTCTGGTGGACTCATCGCAAGATTTACGTCAAGGAGTgc                                                                                                                                  >  3:250698/1‑148 (MQ=255)
                 gTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGAc                                                                                                                                 >  7:309964/1‑148 (MQ=255)
                     tCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGaaaa                                                                                                                            >  3:241312/1‑149 (MQ=255)
                     tCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAACATCCTGATCACTGATACTGGGGTCGAGCCGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                                                                                                                                         >  6:269791/1‑136 (MQ=255)
                     tCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAACATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCaa                                                                                                                                         <  5:269791/136‑1 (MQ=255)
                         aCCTTGTATGCCCTTCCACTTTATGGCTTGCCTTTGGTTGGTTGTTTGGGGGCTCGTTATTACCGATTTCATCCTGTTCAATGTTAATGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGttt                                                                                                                        <  4:250698/149‑1 (MQ=255)
                         aCCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGttt                                                                                                                        <  7:193262/149‑1 (MQ=255)
                         aCCTGGGATGACCTTCCACTTCATGCCTGTCCTTTGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGttt                                                                                                                        <  8:311426/149‑1 (MQ=255)
                          ccTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGttt                                                                                                                        >  7:72434/1‑148 (MQ=255)
                           cTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAGTCCTTGACGAAAAGCTTGc                                                                                                                      <  6:87315/149‑1 (MQ=255)
                           cTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTg                                                                                                                       >  6:295555/1‑148 (MQ=255)
                           cTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGc                                                                                                                      <  3:337111/149‑1 (MQ=255)
                           cTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAACTTTTCGTCAGTCCTTGACGAAAAGCTTGc                                                                                                                      <  7:98494/149‑1 (MQ=39)
                           cTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAACTTTTCGTCAGTCCTTGACGAAAAGCTTGc                                                                                                                      <  7:93716/149‑1 (MQ=39)
                              gTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGTGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCaa                                                                                                                    <  8:100684/148‑1 (MQ=255)
                                  gACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAg                                                                                                                           >  6:347935/1‑137 (MQ=255)
                                  gACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAg                                                                                                                           <  5:347935/137‑1 (MQ=255)
                                    ccTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTg                                                                                                             <  5:126211/149‑1 (MQ=255)
                                     cTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTg                                                                                                             >  1:157345/1‑148 (MQ=255)
                                      ttCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATGACTGATTCTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTg                                                                                                             >  8:132056/1‑147 (MQ=255)
                                         cACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGtt                                                                                                        <  5:191661/149‑1 (MQ=255)
                                          aCTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTta                                                                                                       >  6:339160/1‑149 (MQ=255)
                                          aCTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTta                                                                                                       <  1:135019/149‑1 (MQ=255)
                                           cTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTtat                                                                                                      <  4:286910/149‑1 (MQ=255)
                                           cTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTCACGAAAAGTTTGCAAGTCGCTGGAGTta                                                                                                       >  2:204046/1‑148 (MQ=255)
                                               aTGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTAGCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGt                                                                                                   <  5:295555/148‑1 (MQ=255)
                                               aTGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGt                                                                                                   <  4:241312/148‑1 (MQ=255)
                                                  ccTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCa                                                                                               >  3:227560/1‑149 (MQ=255)
                                                        cTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGc                                                                                                 <  1:346225/141‑1 (MQ=255)
                                                        cTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGc                                                                                                 >  2:346225/1‑141 (MQ=255)
                                                           tggatggaTGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCt                                                                                      <  3:324048/149‑1 (MQ=255)
                                                                 gatgatTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGc                                                                                 >  8:32765/1‑148 (MQ=255)
                                                                 gatgatTGGGGGCTCGAGATTACCAAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGc                                                                                 <  6:302912/148‑1 (MQ=255)
                                                                                gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGc                                                                 >  3:227817/1‑149 (MQ=255)
                                                                                gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGc                                                                 >  5:212426/1‑149 (MQ=255)
                                                                                gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGc                                                                 >  5:168490/1‑149 (MQ=255)
                                                                                gagaTTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTCTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGt                                                                   <  4:227560/147‑1 (MQ=255)
                                                                                         gagaGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCa                                                        <  8:72434/149‑1 (MQ=255)
                                                                                          agagCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCa                                                        >  7:87321/1‑148 (MQ=255)
                                                                                           gagCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCAcc                                                      <  8:309964/149‑1 (MQ=255)
                                                                                           gagCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCAcc                                                      <  5:339160/149‑1 (MQ=255)
                                                                                            agCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCAcc                                                      <  2:157345/148‑1 (MQ=255)
                                                                                              cATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATc                                                   <  4:227817/149‑1 (MQ=255)
                                                                                              cATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCATc                                                   <  5:94455/149‑1 (MQ=255)
                                                                                                   tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCAt                                                    >  7:175541/1‑143 (MQ=255)
                                                                                                   tGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCTTTAAGGCACCAt                                                    <  8:175541/143‑1 (MQ=255)
                                                                                                     aTCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAg                                            <  5:73949/149‑1 (MQ=255)
                                                                                                      tCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGc                                           <  7:32765/149‑1 (MQ=255)
                                                                                                       cACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTtata                                                                                                     >  2:148139/1‑90 (MQ=255)
                                                                                                       cACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTtata                                                                                                     <  1:148139/90‑1 (MQ=255)
                                                                                                          tGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACt                                       >  3:294640/1‑149 (MQ=255)
                                                                                                            aTACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTc                                      <  1:314433/148‑1 (MQ=255)
                                                                                                            aTACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTc                                      >  2:314433/1‑148 (MQ=255)
                                                                                                              aCTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCtt                                   >  4:294485/1‑149 (MQ=255)
                                                                                                              aCTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCtt                                   >  6:168208/1‑149 (MQ=255)
                                                                                                                tGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTg                                 >  1:77595/1‑149 (MQ=255)
                                                                                                                   tgTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAATGAATGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGttt                              <  1:204046/148‑1 (MQ=255)
                                                                                                                     tCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTa                            <  7:132056/149‑1 (MQ=255)
                                                                                                                       gagaCGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTAc                           >  6:233068/1‑148 (MQ=255)
                                                                                                                         gaCGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCCCACCAGCCACTCCTTTGTTTTACaa                         >  6:155525/1‑148 (MQ=255)
                                                                                                                           cGTTCTGCTCGACTCCTTGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATTGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACaaaa                       <  5:155525/148‑1 (MQ=255)
                                                                                                                            gTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACaaaaa                      >  7:299440/1‑148 (MQ=255)
                                                                                                                                 gCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCAc                >  6:83499/1‑149 (MQ=255)
                                                                                                                                  cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACg               <  2:44085/149‑1 (MQ=255)
                                                                                                                                  cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACg               <  6:168490/149‑1 (MQ=255)
                                                                                                                                  cTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACg               >  5:120087/1‑149 (MQ=255)
                                                                                                                                   tCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGtt                               <  2:265532/132‑1 (MQ=255)
                                                                                                                                   tCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGtt                               >  1:265532/1‑132 (MQ=255)
                                                                                                                                    cGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGGACg               >  5:49294/1‑147 (MQ=255)
                                                                                                                                        gacttgCAAACTTTTCGTCAGTCCTTGACGAAAAGCTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCt         >  7:5388/6‑149 (MQ=38)
                                                                                                                                          ctcGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCtt        >  7:96221/1‑148 (MQ=255)
                                                                                                                                             gCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTg       <  2:105787/146‑1 (MQ=255)
                                                                                                                                             gCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTg       >  1:105787/1‑146 (MQ=255)
                                                                                                                                             gCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGa      <  8:96221/147‑1 (MQ=255)
                                                                                                                                             gCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAag    >  7:14640/1‑149 (MQ=255)
                                                                                                                                             gCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCGGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTg       >  7:105001/1‑146 (MQ=255)
                                                                                                                                               aaGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAagag  <  5:83499/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCCCGAGCGACAACAGCGTGCTCAAACCTGGTATGACCTTCCACTTCATGCCTGGCCTATGGATGGATGATTGGGGGCTCGAGATTACCGAGAGCATCCTGATCACTGATACTGGGGTCGAGACGTTCTGCTCGACTCCTCGCAAGCTTTTCGTCAAGGACTGACGAAAAGTTTGCAAGTCGCTGGAGTTATAGTGCCACGGCTATCTTCAGCGACAATGTGATGGTGCCTTAAAGCACCATCACTCCAGCCACTCCTTTGTTTTACAAAAAAAGCACGCAGCCTTGAAGAG  >  NC_002947/5028760‑5029051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑