| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,030,748 | A→G | 7.0% | intergenic (+34/‑461) | PP_4433 → / → dadA‑I | amino acid MFS transporter/D‑amino acid dehydrogenase small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,030,748 | 0 | A | G | 7.0% | 163.8 / 2.2 | 57 | intergenic (+34/‑461) | PP_4433/dadA‑I | amino acid MFS transporter/D‑amino acid dehydrogenase small subunit |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base G (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GCACCTGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCTTG > NC_002947/5030601‑5030896 | gCACCTGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGC‑AAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAAt > 2:71925/1‑149 (MQ=255) ccTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCAc > 6:65705/1‑149 (MQ=255) tactacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAAGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCAcc > 2:76016/1‑148 (MQ=255) ctacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTTATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCAccc < 7:19565/147‑1 (MQ=255) ctacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTGATTA‑GCACCCAg < 8:32299/149‑1 (MQ=255) tacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAgg > 7:231902/1‑149 (MQ=255) tacATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAg > 1:69937/1‑148 (MQ=255) tGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGc > 8:54765/1‑149 (MQ=255) ggTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAAt > 7:130578/1‑130 (MQ=255) ggTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAAt < 8:130578/130‑1 (MQ=255) gTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCg > 7:108915/1‑148 (MQ=255) aTCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATc > 6:120163/1‑125 (MQ=255) aTCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATc < 5:120163/125‑1 (MQ=255) aTCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTc < 5:65705/149‑1 (MQ=255) tCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTc > 1:310010/1‑148 (MQ=255) gtattattGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGTTTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCg < 6:19/146‑1 (MQ=255) gtttattGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTATTAGGCAGATGGTATATAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCgg < 1:218983/146‑1 (MQ=255) gttgttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGAc > 5:54993/1‑149 (MQ=255) gttgttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGC‑AAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCACGCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGAc < 1:71925/149‑1 (MQ=255) ttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATtcctc < 2:316400/132‑1 (MQ=255) ttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATtcctc > 1:316400/1‑132 (MQ=255) ttCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCgg < 6:250558/148‑1 (MQ=255) cTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCt < 8:108915/148‑1 (MQ=255) gtgATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTg < 5:90129/110‑1 (MQ=255) gtgATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTg > 6:90129/1‑110 (MQ=255) gtgATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTc > 8:314901/1‑149 (MQ=255) tCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGTCCGGCTACGTCGat < 1:130402/148‑1 (MQ=255) agaCTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATaa < 8:231902/149‑1 (MQ=255) aaGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAagcag > 7:144546/1‑147 (MQ=255) gTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAAAAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCAATCCTCCGGGCAAGCCGACCGCCTCCGGCGATATAAGCAGCAgcg > 2:242135/1‑149 (MQ=255) gCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTc > 8:288446/1‑149 (MQ=255) tCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAagcagca < 4:7624/141‑1 (MQ=255) tCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAagcagca > 3:7624/1‑141 (MQ=255) gATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCAAAGCTCCATTCCTCCGGGCaa > 2:179832/1‑110 (MQ=255) gATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCAAAGCTCCATTCCTCCGGGCaa < 1:179832/110‑1 (MQ=255) gggATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCttt < 2:310010/149‑1 (MQ=255) aaTACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCt > 4:60392/1‑149 (MQ=255) aTACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCtt < 4:296031/149‑1 (MQ=255) cTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTAtct < 7:314901/149‑1 (MQ=255) tGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTAtctc > 5:131532/1‑149 (MQ=255) gCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCC‑‑TTTATTCTTATCTCACctct > 3:297313/1‑149 (MQ=255) aTAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTgg < 8:144546/149‑1 (MQ=255) ggCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGccc < 7:288446/148‑1 (MQ=255) gCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCt > 6:116769/1‑148 (MQ=255) cAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGa < 3:60392/149‑1 (MQ=255) aGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGAt < 2:18811/149‑1 (MQ=255) aTGGTTTAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATACGCAGCTGCGCTCCAGCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGAt < 1:242135/147‑1 (MQ=255) tGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCC‑‑TTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAg < 4:297313/149‑1 (MQ=255) gTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACa < 5:116769/148‑1 (MQ=255) gTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACa < 7:54765/148‑1 (MQ=255) tataGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATGTCACCTCTTGGCATCTGGCCCTGATAACAgt > 8:215972/1‑149 (MQ=255) gCCTGGGTCCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTAATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCtt < 7:215972/148‑1 (MQ=255) cTGGGTGCTGATTA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGTGCTCCACCCTTCCTTTTTAtt < 2:28501/104‑1 (MQ=255) cTGGGTGCTGATTA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGTGCTCCACCCTTCCTTTTTAtt > 1:28501/1‑104 (MQ=255) cTGGGTGCTGATTA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGc > 6:198202/1‑149 (MQ=255) cTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGc > 6:110433/1‑149 (MQ=255) tAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCtt > 5:107633/1‑149 (MQ=255) aaTCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCTTg > 2:47175/1‑149 (MQ=255) | GCACCTGCCTACTACATCATGGTTGCATCAGCACTTGGGATTATTGCGTTGTTCTTTGTGATCGAGACTAAAGGTTGCTCGATTCGCGGGGATGGAATACCTGGGGCAAAATAACCTAATAGGCAGATGGTATAGAGCCTGGGTGCTAATCA‑GCACCCAGGCTGTTGCGAAGCTCCATTCCTCCGGGCAAGCCGACCGGCTACGTCGATATAAGCAGCAGCGCTCCACCCTTCCTTTTTATTCTTATCTCACCTCTTGGCATCTGGCCCTGATAACAGTGTCTTCGCTATCTCTTG > NC_002947/5030601‑5030896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |