Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 71,791 A→T 13.0% A38A (GCT→GCA)  glyS ← glycine‑tRNA ligase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294771,7910AT13.0% 174.5 / 15.4 69A38A (GCT→GCA) glySglycine‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (33/27);  new base T (6/3);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GGCTCGCCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGT  >  NC_002947/71645‑71935
                                                                                                                                                  |                                                                                                                                                
ggCTCGCCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCcagc                                                                                                                                                <  3:51070/149‑1 (MQ=255)
ggCTCGCCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCcag                                                                                                                                                 <  2:165070/148‑1 (MQ=255)
 gCTCGCCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCcagca                                                                                                                                               <  6:69545/149‑1 (MQ=255)
  cTCGCCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCcagcag                                                                                                                                              >  8:23976/1‑149 (MQ=255)
      ccTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGTTGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                          >  1:244808/1‑149 (MQ=255)
       cTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGc                                                                                                                                         <  4:109864/149‑1 (MQ=255)
        ttCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                          >  8:304937/1‑147 (MQ=255)
                  aaGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCttttt                                                                                                                               <  7:304937/148‑1 (MQ=255)
                     gCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGGCTGCTGGCCt                                                                                                                                   <  4:203101/141‑1 (MQ=255)
                     gCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGGCTGCTGGCCt                                                                                                                                   >  3:203101/1‑141 (MQ=255)
                            gCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGc                                                                                                                     >  4:280568/1‑148 (MQ=255)
                               ggggCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAg                                                                                                                  >  8:28812/1‑148 (MQ=255)
                                gggcgggcCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAgg                                                                                                                 >  5:283228/1‑148 (MQ=255)
                                 ggcgggcCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGaa                                                                                                               <  7:216321/149‑1 (MQ=255)
                                     ggcCGTCGATGTAGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGCCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCg                                                                                                           >  1:112195/1‑149 (MQ=255)
                                         gTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGc                                                                                                       >  8:229166/1‑149 (MQ=255)
                                                ttGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCagcctt                                                                                                >  3:30986/1‑144 (MQ=255)
                                                 tGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTg                                                                                               >  1:1911/1‑149 (MQ=255)
                                                      ctgcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                          >  2:216595/1‑101 (MQ=255)
                                                      ctgcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                          <  1:216595/101‑1 (MQ=255)
                                                       tgcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGTCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCATCCTGCAGGCCTTTTTCGATAACGTCCAGGAAGGCGTCTCCAAGGCTGGCGAgg                                                                                         <  1:320641/149‑1 (MQ=255)
                                                        gcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTGTTTCGATat                                                                                                                         >  4:167952/1‑115 (MQ=255)
                                                         cGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAggg                                                                                        <  6:283228/148‑1 (MQ=255)
                                                             cAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTg                                                                                   >  7:111054/1‑149 (MQ=255)
                                                                gTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTgg                                                                                  >  7:312763/1‑147 (MQ=255)
                                                                gTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGt                                                                                <  2:112195/149‑1 (MQ=255)
                                                                gTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGt                                                                                <  1:289490/149‑1 (MQ=255)
                                                                   gctgcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTCTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCACGGCTGGCGAGGGCCTTGGGTgg                                                                              >  7:301710/1‑148 (MQ=255)
                                                                      gcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGc                                                                                                       >  7:257186/1‑120 (MQ=255)
                                                                      gcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGc                                                                                                       <  8:257186/120‑1 (MQ=255)
                                                                      gcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGc                                                                          >  7:65731/1‑149 (MQ=255)
                                                                       cACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGc                                                                          <  7:23976/148‑1 (MQ=255)
                                                                           tCCAGCTGGCGACTCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTc                                                                     <  2:1911/149‑1 (MQ=255)
                                                                             cAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCg                                                                    <  8:111054/148‑1 (MQ=255)
                                                                              aGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGt                                                                  <  2:244808/149‑1 (MQ=255)
                                                                              aGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGt                                                                  >  5:51400/1‑149 (MQ=255)
                                                                              aGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGt                                                                  >  5:27377/1‑149 (MQ=255)
                                                                                   gCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGAGGCAGCTCTTCGGTGccc                                                              <  8:312763/148‑1 (MQ=255)
                                                                                    cGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAg                                                            >  7:238893/1‑149 (MQ=255)
                                                                                     gAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGt                                                           >  4:211033/1‑149 (MQ=255)
                                                                                      aaTCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGcc                                                               <  5:81497/144‑1 (MQ=255)
                                                                                      aaTCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGcc                                                               >  6:81497/1‑144 (MQ=255)
                                                                                        tCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTc                                                         >  4:294637/1‑148 (MQ=255)
                                                                                          aGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTAc                                                      <  6:27377/149‑1 (MQ=255)
                                                                                          aGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTAc                                                      <  7:229166/149‑1 (MQ=255)
                                                                                                    gacgacGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCt                                            <  3:280568/149‑1 (MQ=255)
                                                                                                     acgacgCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCtt                                           >  8:150343/1‑149 (MQ=255)
                                                                                                          gcggcgcggcGTACACCTGCTTGCGGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCa                                      >  5:196917/1‑149 (MQ=255)
                                                                                                              cgcggcgTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCa                                  <  7:28812/149‑1 (MQ=255)
                                                                                                               gcggcgTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAt                                 >  6:236967/1‑149 (MQ=255)
                                                                                                                      acacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAg                          >  7:79532/1‑149 (MQ=255)
                                                                                                                       cacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGc                         <  5:236967/149‑1 (MQ=255)
                                                                                                                       cacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGc                         <  8:238893/149‑1 (MQ=255)
                                                                                                                       cacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGc                         <  3:294637/149‑1 (MQ=255)
                                                                                                                        acCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTgg                                                                                  >  6:100919/1‑91 (MQ=255)
                                                                                                                        acCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTgg                                                                                  <  5:100919/91‑1 (MQ=255)
                                                                                                                        acCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGcc                        >  5:191425/1‑149 (MQ=255)
                                                                                                                          cTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTc                      >  6:276921/1‑149 (MQ=255)
                                                                                                                            gCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGa                                         <  4:212697/128‑1 (MQ=255)
                                                                                                                            gCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGa                                         >  3:212697/1‑128 (MQ=255)
                                                                                                                              ttGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGc                  >  4:258158/1‑149 (MQ=255)
                                                                                                                                gCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGc                  <  3:211033/147‑1 (MQ=255)
                                                                                                                                 ccGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTa               <  6:119969/149‑1 (MQ=255)
                                                                                                                                       tAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCaa          >  5:163964/1‑148 (MQ=255)
                                                                                                                                       tAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAac         <  8:79532/149‑1 (MQ=255)
                                                                                                                                        gaaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                <  4:48989/120‑1 (MQ=255)
                                                                                                                                        gaaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAaca        >  1:44653/6‑149 (MQ=255)
                                                                                                                                        gaaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCGTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                >  3:48989/6‑125 (MQ=255)
                                                                                                                                         aaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAacac       >  4:16572/5‑149 (MQ=255)
                                                                                                                                          ttCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                >  2:99630/1‑123 (MQ=255)
                                                                                                                                          ttCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                <  1:99630/123‑1 (MQ=255)
                                                                                                                                          ttCAGGACAGCAGCCTGCAGGCCTTTTTCGATCCCGGCCAGGAAGGCGTCGCCAACGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTAACAGGAAATCTTGAGCGCTCATTGTGCAGCCTCAAGCTTAGACAaccc       >  2:251609/1‑146 (MQ=255)
                                                                                                                                              ggCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGt  >  4:259213/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGCTCGCCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGT  >  NC_002947/71645‑71935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑