Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	427,376	G→T	6.1%	A141A (GCC→GCA)	PP_0350 ←	ferrichrome‑iron receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	427,376	0	G	T	6.1%	212.5 / 3.7	66	A141A (GCC→GCA)	PP_0350	ferrichrome‑iron receptor
Reads supporting (aligned to +/- strand):  ref base G (36/26);  new base T (2/2);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

GCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCGGTACCG  >  NC_002947/427230‑427523                                                                                                                                                   |                                                                                                                                                    gCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTgg                                                                                                                                                    <  1:14495/148‑1 (MQ=255) gCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGc                                                                                                                                                   >  5:83457/1‑149 (MQ=255)  cAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGcc                                                                                                                                                  <  2:342072/149‑1 (MQ=255)  cAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGc                                                                                                                                                   >  1:161199/1‑148 (MQ=255)           tGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCt                                                                                                                                         <  5:227153/149‑1 (MQ=255)             cccGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGCGCGGGGGACTTCCACAAGTGCCGTGTCAGGCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCtt                                                                                                                                        <  1:58110/148‑1 (MQ=255)               cGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCaa                                                                                                                                     >  5:103353/1‑149 (MQ=255)                   atatAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCgg                                                                                                                                 >  1:274770/1‑149 (MQ=255)                    tataGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCggg                                                                                                                                <  2:109669/149‑1 (MQ=255)                      taGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGcc                                                                                                                              >  1:44694/1‑149 (MQ=255)                                catcatCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCtt                                                                                                                    >  7:155795/1‑149 (MQ=255)                                catcatCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCtt                                                                                                                    >  6:217120/1‑149 (MQ=255)                                  tcatcCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCAACGTAGCCTTCAACCGGGc                                                                                                                               >  1:136987/1‑136 (MQ=255)                                    atcCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTTGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTcgc                                                                                                                >  5:233535/1‑149 (MQ=255)                                    atcCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTTGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTcgc                                                                                                                >  5:228454/1‑149 (MQ=255)                                     tcCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTcgct                                                                                                               >  6:125686/1‑149 (MQ=255)                                           cTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTcgctg                                                                                                              <  6:223359/144‑1 (MQ=255)                                           cTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTcgctg                                                                                                              >  5:223359/1‑144 (MQ=255)                                              tgtACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCtggt                                                                                                      <  5:125686/149‑1 (MQ=255)                                                 acacCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCtggtgg                                                                                                    >  6:306751/1‑148 (MQ=255)                                                   acCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCAt                                                                                                 >  3:64747/1‑149 (MQ=255)                                                   acCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCAt                                                                                                 <  6:83457/149‑1 (MQ=255)                                                   acCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTAGCTGGCGCTGGTGGCAt                                                                                                 >  3:69389/1‑149 (MQ=255)                                                      gcgGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCa                                                                                              <  2:44694/149‑1 (MQ=255)                                                      gcgGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCa                                                                                              >  4:31029/1‑149 (MQ=255)                                                         ggcTTGCAGTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGAGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACgg                                                                                           <  4:263592/147‑1 (MQ=255)                                                         gTCTTTCATTTGCTCGCGGTTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTTGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACgg                                                                                           <  6:228454/149‑1 (MQ=255)                                                         gTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTTGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACgg                                                                                           <  6:233535/149‑1 (MQ=255)                                                         gTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACgg                                                                                           <  5:12021/149‑1 (MQ=255)                                                         gTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACgg                                                                                           <  5:306751/149‑1 (MQ=255)                                                            tttCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTACGCTGGCGCTGGTGGCATTCACggtgg                                                                                        >  8:212061/1‑149 (MQ=255)                                                              tCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACggtg                                                                                         <  8:155795/146‑1 (MQ=255)                                                              tCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACg                                                                                            >  5:52928/1‑143 (MQ=255)                                                              tCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACg                                                                                            <  6:52928/143‑1 (MQ=255)                                                                aTTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTc                                                                                    >  4:139516/1‑149 (MQ=255)                                                                 tttGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCtggtg                                                                                                     <  6:18213/131‑1 (MQ=255)                                                                      tCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGc                                                                                >  8:57360/1‑147 (MQ=255)                                                                        gcgGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCGACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAgg                                                                            >  5:38972/1‑149 (MQ=255)                                                                           ggTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGc                                                                           >  8:86519/1‑147 (MQ=255)                                                                             tGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAgt                                                                       >  1:334052/1‑149 (MQ=255)                                                                                  aCAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCATCCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGcc                                                                  <  5:217120/149‑1 (MQ=255)                                                                                    agagACGGAGCGGGGGGCTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCAt                                                                >  7:117576/1‑149 (MQ=255)                                                                                      agaCGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGtgtg                                                                    <  3:232099/143‑1 (MQ=255)                                                                                      agaCGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGtgtg                                                                    >  4:232099/1‑143 (MQ=255)                                                                                      agaCGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATcc                                                              >  5:186578/1‑149 (MQ=255)                                                                                         cGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGc                                                           <  6:103353/149‑1 (MQ=255)                                                                                            aGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACg                                                        <  2:161199/149‑1 (MQ=255)                                                                                                      ttCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGc                                              >  1:205256/1‑149 (MQ=255)                                                                                                      ttCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGc                                              >  1:277254/1‑149 (MQ=255)                                                                                                      ttCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGc                                              >  6:182631/1‑149 (MQ=255)                                                                                                       tCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCt                                             >  8:29741/1‑149 (MQ=255)                                                                                                                     gtCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGAGGCATTCACGGTGGAGACCGGAAAGGCCAGTGTGCCATCCGGCACGGCCACCAGTCTGTAGCTGCCCGCAc                               >  4:215741/1‑149 (MQ=255)                                                                                                                      tCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCATGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCAct                              >  8:12016/1‑149 (MQ=255)                                                                                                                      tCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCa                                                          >  5:174507/1‑121 (MQ=255)                                                                                                                      tCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCa                                                          <  6:174507/121‑1 (MQ=255)                                                                                                                         gTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCACGCGCTTTCGCTGGCGCTGGTGGCAATCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCActgct                           <  2:274770/149‑1 (MQ=255)                                                                                                                            ttGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTAACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCActgctctg                        >  5:261581/1‑149 (MQ=255)                                                                                                                              ggTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAgg                                               <  2:56756/124‑1 (MQ=255)                                                                                                                              ggTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAgg                                               >  1:56756/1‑124 (MQ=255)                                                                                                                                tGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTctgctg                     >  2:208382/1‑148 (MQ=255)                                                                                                                                 gCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCtgctgc                    >  7:134435/1‑148 (MQ=255)                                                                                                                                        gcGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAg            <  5:182631/149‑1 (MQ=255)                                                                                                                                          ggTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGc          <  3:31029/149‑1 (MQ=255)                                                                                                                                            tGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCg        <  3:139516/149‑1 (MQ=255)                                                                                                                                                cTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCGGTAc    <  4:69389/149‑1 (MQ=255)                                                                                                                                                cTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCGGTAc    <  4:64747/149‑1 (MQ=255)                                                                                                                                                 ttGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCGGTAcc   >  4:297178/1‑149 (MQ=255)                                                                                                                                                   ggCGACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCGGTACCg  <  6:38972/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCAGAGACGATGCCCGGCATATAGCGCACTGCATCATCCAGGCTGTGTACACCGCGGTCTTTCATTTGCTCGCGGGTGGCGACAGAGACGGAGCGGGGGACTTCCACAAGTGCCGTGTCAGTCTTGGTGCCAGCAGCGGTGCGCTTGGCCACGTAGCCTTCAACCGGGCCCCAGGCGCTTTCGCTGGCGCTGGTGGCATTCACGGTGGTTTCCGGCAAGGCCAGTGTGCCATCCGGCACGGCCACCAGGCTGTAGCTGCCCGCACTGCTCTGCTGCAACTGCAGGCCGGTACCG  >  NC_002947/427230‑427523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑