| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,097,250 | G→C | 11.6% | intergenic (+241/+51) | PP_mr66 → / ← acsA‑I | P6/acetyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,097,250 | 0 | G | C | 11.6% | 115.5 / 6.7 | 43 | intergenic (+241/+51) | PP_mr66/acsA‑I | P6/acetyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (23/15); new base C (2/3); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01 | |||||||||||
ATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTAC > NC_002947/5097102‑5097397 | aTGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTg < 3:305026/149‑1 (MQ=255) gCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAAGCTGTGCGGTGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGa < 2:35588/148‑1 (MQ=255) cACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGAc < 6:320731/148‑1 (MQ=255) cGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAGAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgcg < 7:222303/149‑1 (MQ=18) ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TGGGCTGAACTGCCGCATTCGCggg > 4:98277/1‑149 (MQ=255) ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg < 3:212335/149‑1 (MQ=255) ggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGACCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCggg < 4:113354/149‑1 (MQ=255) aTTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGccc < 7:234836/149‑1 (MQ=255) gTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGc < 3:98277/148‑1 (MQ=255) cGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc > 6:160903/1‑112 (MQ=14) cGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTcgc < 5:160903/112‑1 (MQ=14) gggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGGCAGCG‑TAGGCGGAACAGCCGCATTAGCGGGGAATCCCGCACCAGCAGGGGGGTTTGCCGGGAAggg > 4:206392/1‑149 (MQ=255) aGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGGGAGGTGAGGGGACAGCG‑TAGCCTGAACTGCCGCATTAGCGGGCCAGCCCCCGCCGGCAGGGGGTTTTGCCGGGAAGGGTTTTCGCCg > 8:43444/1‑147 (MQ=255) gAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa > 5:130468/1‑134 (MQ=255) cAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAAATGAAGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTAAGCCGAAGc > 5:340414/1‑147 (MQ=255) aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGGGAGGGCAGGGGGCCGGG‑TTGCCGGGACAGGCGCCTTAGCGGGGAAGCCCGCGCCGGGAGGGGGGGTTGGCGGGGTGGGGTTTCGGCGTCGCCAgggtg > 6:194302/1‑146 (MQ=255) aaTCCAAACGCGGGCTGCCCCGCTAAGGCGTCAGGCCGGGGCACACCT‑TCCCCTCACCTGCCGCTTGCGCGGCCAACCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGAt < 7:289557/149‑1 (MQ=255) aaaaGCGGGCTTGCCCGCGAATGCGTGAGGTGAGGGGACAGCG‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTGCTGGAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCgtgt > 3:298107/1‑134 (MQ=39) aacgggggCTTGCCCGCTAAGGGGTGAGTTCAGGGCACCGCT‑TAGCCTAAACTGACGCATTGGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTg < 6:340414/144‑1 (MQ=255) aaGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGc > 3:242275/1‑149 (MQ=255) gCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTg > 1:45323/1‑149 (MQ=255) gggCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTa > 1:96479/1‑149 (MQ=255) ccGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc > 8:7218/1‑149 (MQ=255) cgcgAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCg > 6:20557/1‑149 (MQ=255) gctaaTGCGTGAGGGCAGGGGACTGAT‑TTGCCTTAACTGACGTATTCGGGGGAAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg < 2:77404/146‑1 (MQ=255) gcgAATGCGTCAGTTCAGGCTA‑AGCTGTCCCCTGACCTCACGCATTCGCGGGCAAGCCCGCTTTTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCgg > 6:116818/1‑149 (MQ=18) gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc > 1:43898/1‑148 (MQ=255) gTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc > 1:230204/1‑148 (MQ=255) aGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga < 2:43898/148‑1 (MQ=255) gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgaga > 4:230055/1‑148 (MQ=255) gggACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGat < 5:20557/149‑1 (MQ=255) cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGGAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc > 6:137026/1‑148 (MQ=255) cT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGCTCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGcc > 8:309898/1‑148 (MQ=255) tccccTGACCTCACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAg < 1:212973/146‑1 (MQ=255) gCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCa < 1:213441/101‑1 (MQ=255) gCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCa > 2:213441/1‑101 (MQ=255) ccTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTAt < 5:309431/94‑1 (MQ=255) ccTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTAt > 6:309431/1‑94 (MQ=255) ccTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGc > 1:168508/1‑149 (MQ=255) cTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcg > 5:149097/1‑149 (MQ=255) acatGACGAATTCGCGGGCACGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtcg < 4:242275/146‑1 (MQ=255) aCTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGcgtcg < 3:230055/148‑1 (MQ=255) tGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTAc > 7:258755/1‑149 (MQ=255) tGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTAc > 3:201768/1‑149 (MQ=255) | ATGCACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCT‑TAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAGATATCGCCCAGGGCGTCGTAC > NC_002947/5097102‑5097397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |