| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,100,955 | C→A | 8.0% | M224I (ATG→ATT) ‡ | phhR ← | sigma‑54 dependent transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,100,955 | 0 | C | A | 8.0% | 190.5 / 4.2 | 61 | M224I (ATG→ATT) ‡ | phhR | sigma‑54 dependent transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (33/23); new base A (3/2); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATCGACTCGGGCAGCCCGGCGCAGTTGA‑GCGCCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCGCGCCGGCCAG > NC_002947/5100813‑5101101 | ccATCGACTCGGGCAGCCCGGCGCAGTTGAGGCGCCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATgcg > 1:206568/1‑149 (MQ=255) cATCGACTCGGGCAGCCCGGCGCAGTTGA‑GCGCCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGcg‑cc > 1:174096/1‑149 (MQ=255) tCGACTCGGGCAGCCCGGCGCAGTTGA‑GCGCCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCACCATGcg‑ccgc > 6:224968/1‑149 (MQ=255) cTCGGGCAGCCCGGCGCAGTTGA‑GCGCCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACTGTGCATCCAGCGGCGCATGGcg‑ccgctgga < 7:293057/149‑5 (MQ=255) gggCAGCCCGGCGCAGTTGA‑GCGCCATCAGCGGCTACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTc < 2:256706/147‑1 (MQ=255) cgcCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCAccc < 8:147781/149‑1 (MQ=255) gcCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCcg < 6:104119/149‑1 (MQ=255) cAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGc > 4:89618/1‑149 (MQ=255) cccACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCAGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGc > 1:77611/1‑149 (MQ=255) ccACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCt > 4:34083/1‑149 (MQ=255) ccACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCt > 1:129207/1‑149 (MQ=255) ggggTGGCCAGGGGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTGCGCCCTCGATCAGCAACGGGGCAGCCTGCGGCGCCAGGCG‑CGGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGaa < 6:211091/149‑1 (MQ=255) cTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGcc < 2:206568/149‑1 (MQ=255) cAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACg > 7:325186/1‑147 (MQ=255) ggTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGaa > 3:17718/1‑127 (MQ=255) gcaCGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGCCCCCATGCG‑CCGCGCTACACGCACCACGGCAGCCA‑TCACCCGCGACCACTGGAAGATGCTGACGAAGCCACGCAGCActtcctt > 2:303852/1‑149 (MQ=255) cgcgCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTgcg < 3:89618/149‑1 (MQ=255) gcCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCt > 7:245894/1‑119 (MQ=255) gcCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCt < 8:245894/119‑1 (MQ=255) gCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCCCCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTg > 6:210500/1‑149 (MQ=255) cAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGt < 1:872/149‑1 (MQ=255) aaCTCCTTGCTGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTg > 2:265699/1‑147 (MQ=255) aaCTCCTTGCTGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTa > 8:176178/1‑149 (MQ=255) cTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGAt < 8:325186/147‑1 (MQ=255) cTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATAc > 6:108999/1‑149 (MQ=255) cTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATAc > 5:266922/1‑149 (MQ=255) ttGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACg > 5:321129/1‑149 (MQ=255) gCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCt > 2:76325/1‑149 (MQ=255) gttccggttTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGt < 8:24968/103‑1 (MQ=255) gttccggttTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGt > 7:24968/1‑103 (MQ=255) tttCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGa < 5:316129/79‑1 (MQ=255) tttCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGa > 6:316129/1‑79 (MQ=255) cGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTc < 1:265699/148‑1 (MQ=255) cGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTc < 7:176178/148‑1 (MQ=255) gCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCgg < 2:77611/149‑1 (MQ=255) cccTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGAt < 8:40217/140‑1 (MQ=255) cccTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGAt > 7:40217/1‑140 (MQ=255) ccTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCGTCCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCgg > 1:62070/1‑148 (MQ=255) ccTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGa < 5:114595/84‑1 (MQ=255) ccTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGa > 6:114595/1‑84 (MQ=255) ccTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCgg < 2:62070/147‑1 (MQ=255) tCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACg > 4:295958/1‑149 (MQ=255) aTCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTg > 1:311575/1‑149 (MQ=255) aGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGctct > 8:209905/1‑99 (MQ=255) aGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGctct < 7:209905/99‑1 (MQ=255) aGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCa < 2:311575/148‑1 (MQ=255) gCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCATTCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAg > 1:90699/1‑149 (MQ=255) cAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCgg < 1:327823/137‑1 (MQ=255) cAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCgg > 2:327823/1‑137 (MQ=255) cATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGt > 5:262699/1‑126 (MQ=255) cATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGt < 6:262699/126‑1 (MQ=255) aagcgcggcgCATGGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCCGTCGGCACCGTGCAGGGTAAGCACcgc > 8:318438/5‑149 (MQ=255) ccAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACcgcg > 8:146894/1‑149 (MQ=255) ccAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACCCAGCTCTTGCTTGCGCACATTGTAGATACCCTCGCCGATCCGGTCGGCACGGTGCAGGCTAAGCACcgcg > 3:185458/1‑149 (MQ=255) gcgcggcgCATGGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCgcgc > 1:294578/3‑149 (MQ=255) gcggcgCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCGCGCCg < 5:108999/149‑1 (MQ=255) gcggcgCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCGCGCCg > 7:218170/1‑149 (MQ=255) gcggcgCATGGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGc > 5:40546/1‑131 (MQ=255) gcggcgCATGGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGc < 6:40546/131‑1 (MQ=255) cgcCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCGCGCCGGCCa < 5:210500/149‑1 (MQ=255) gcCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCGCGCCGGCCAg > 6:122416/1‑149 (MQ=255) | CCATCGACTCGGGCAGCCCGGCGCAGTTGA‑GCGCCATCAGCGGCGACTGCCCACGCGGGCTGGCCAGGTGGCAGGCACGCGCCAGCAACTCCTTGCCGGTTCCGGTTTCGCCCTCGATCAGCAACGGTGCATCCAGCGGCGCCATGCG‑CCGCGCTTCACGCACCACGGCAGCCA‑TCACCCGCGAGCTCTGGAAGATGCTGTCGAAGCCACGCAGCTCTTGCTTGCGCACATTGTAGATACGCTCGCCGATCCGGTCGGCACGGTGCAGGGTAAGCACCGCGCCGGCCAG > NC_002947/5100813‑5101101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |