| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,173,244 | T→C | 9.2% | intergenic (‑285/+47) | fadD‑I ← / ← fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,173,244 | 0 | T | C | 9.2% | 145.2 / 4.1 | 54 | intergenic (‑285/+47) | fadD‑I/fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (26/23); new base C (3/2); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GAGGCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTG > NC_002947/5173097‑5173390 | gagGCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACAtt > 1:187622/1‑149 (MQ=255) ggCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGCGCATTCGCGGGTAAACCCGCTCCTACAtt < 1:25882/148‑1 (MQ=255) ggCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTg‑g < 2:284759/149‑1 (MQ=255) cAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTg‑gg > 8:86152/1‑147 (MQ=255) aaaaCAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAg > 5:172591/1‑149 (MQ=255) cTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGc < 1:274984/149‑1 (MQ=255) cTTATGGTCACAAAAATGACTCAATGAACAATACAGCTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACACTG‑CCCAATGTAGGAGc < 2:43492/149‑1 (MQ=39) ttATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGATAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCg > 5:132368/1‑149 (MQ=255) tCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTTAAACCCCAACTAAATTG‑TGCAGTGTATGACGGGG‑ttt > 6:53406/1‑148 (MQ=255) tCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTa > 2:178504/1‑149 (MQ=255) tCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTa < 6:172591/149‑1 (MQ=255) cacaAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTAc > 1:73158/1‑149 (MQ=255) acaAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTAcc < 2:73158/149‑1 (MQ=255) aaTGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑GTTACCCGCGAAGAAGcc > 3:180707/1‑148 (MQ=255) gAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATcc > 2:111920/1‑149 (MQ=255) aaTACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGt < 7:86152/149‑1 (MQ=255) aGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCAc > 7:134448/1‑149 (MQ=255) gTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGc > 6:332906/1‑149 (MQ=255) gagTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGATAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg < 6:132368/148‑1 (MQ=255) tCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCcgcg < 8:81622/107‑1 (MQ=255) tCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCcgcg > 7:81622/1‑107 (MQ=255) tCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCccgc > 7:202496/1‑149 (MQ=255) aaaaTGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GAATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCccgccg > 1:133100/1‑148 (MQ=255) tggCATATAAGGCTGTATAGAGCGTGTTAGAGCGGTTTACG‑GCATGCGGGGTTAAACCCGCTCGTACATTG‑GGCCGTGTCGTAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAAt < 1:178504/146‑1 (MQ=255) gCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCtt < 8:134448/149‑1 (MQ=255) gCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCtt > 1:266203/1‑149 (MQ=255) aGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGGTTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTg < 1:271248/149‑1 (MQ=255) aGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGc < 8:1196/149‑1 (MQ=255) aGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGc < 2:133100/149‑1 (MQ=255) ggCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGGGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg < 1:246272/120‑1 (MQ=255) ggCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATcgcg > 2:246272/1‑120 (MQ=255) ggCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATc < 3:34807/139‑1 (MQ=255) ggCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATc > 4:34807/1‑139 (MQ=255) gTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTg > 7:80310/1‑138 (MQ=255) gTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTg < 8:80310/138‑1 (MQ=255) gagCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTGCGTCCGGGATACCCCCAC‑AATACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATg < 1:111920/149‑1 (MQ=255) tGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACTGGCGTCATGGGCAAc > 1:217325/1‑149 (MQ=255) gCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgc > 3:88731/1‑149 (MQ=255) tttAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCgcag > 4:27280/1‑149 (MQ=255) aaG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATgg < 2:264225/130‑1 (MQ=255) aaG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATgg > 1:264225/1‑130 (MQ=255) aG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGAATCGCGCAGCAc > 5:348811/1‑148 (MQ=255) gcgGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCtt < 2:266203/149‑1 (MQ=255) ggggAAACCCGCTCCTACTTTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTgg < 3:83698/149‑1 (MQ=255) gggTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTgg < 8:202496/149‑1 (MQ=255) gTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTggg > 5:280795/1‑148 (MQ=255) tAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTa > 1:133097/1‑149 (MQ=255) aaaCCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgcg < 4:67560/128‑1 (MQ=255) aaaCCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgcg > 3:67560/1‑128 (MQ=255) cacGCTCCTACACTG‑CCCAATGTAGGAGCGGG‑TTTACACGCGAAGATGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACttt > 3:120473/3‑149 (MQ=38) ccGCTCCTACACTG‑CCCAATGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTg > 8:210707/1‑149 (MQ=255) gCTCCTACACTG‑CCCAATGTAGGAGCGGG‑TTTACCCGCGAATAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCAGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTg > 3:266300/1‑147 (MQ=38) cTACACTG‑CCCAATGTAGGAGCGGG‑TTTACCCGCGAATAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGcc < 4:266300/148‑1 (MQ=39) cATTG‑GTCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCgg < 4:88731/147‑1 (MQ=255) cATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTg < 5:332906/149‑1 (MQ=255) aTTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGACGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAAACTTGCCCACCCGCGTCATGGGCACCGATTCGCGCAGCACGATGTGCTTGGGTACATTGAAGCCGGtc > 2:34990/1‑147 (MQ=255) | GAGGCCAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAG‑GCATTCGCGGGTAAACCCGCTCCTACATTG‑GGCAGTGTAGGAGCGGG‑TTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTG > NC_002947/5173097‑5173390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |