Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,180,012 C→G 9.0% intergenic (‑240/‑43) PP_4557 ← / → PP_4558 hypothetical protein/GNAT family acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,180,0120CG 9.0% 151.3 / 4.9 53intergenic (‑240/‑43)PP_4557/PP_4558hypothetical protein/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base G (2/3);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

GTACCGATCCCGCTTCGTACTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTA  >  NC_002947/5179868‑5180159
                                                                                                                                                |                                                                                                                                                    
gTACCGATCCCGCTTCGTACTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCctct                                                                                                                                                  <  5:132169/149‑1 (MQ=255)
       tCCCGCGTCGTACTGCCAGGGGGGATGAACAAACTTCTACTCCCGGCCAGCAATAGTTGAACCCCGTCGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGGCTCTGGCCtga                                                                                                                                           <  5:241774/149‑3 (MQ=255)
          cGCTTCGTACTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGtt                                                                                                                                         >  1:2490/1‑148 (MQ=255)
            cTTCGTACTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTACGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCa                                                                                                                                      <  1:253740/149‑1 (MQ=255)
               cGTACTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAgg                                                                                                                                    <  4:12300/148‑1 (MQ=255)
                 tACTGCCTGGGGGTATGAACAAAATTCTACTCCCGGCAAGCAATAGTTTAACCCCGTCGTCGGATATTCACCCTTGCCGGTTTTGCAAAAATTTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTa                                                                                                                                 <  1:93952/149‑1 (MQ=255)
                  aCTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACACTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCTCAAATCATCAGGCCACAGCCTATGGCCTCGTTTCAGCGTAg                                                                                                                                >  6:101701/1‑149 (MQ=255)
                      ccAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGt                                                                                                                                          <  4:111575/135‑1 (MQ=255)
                      ccAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGt                                                                                                                                          >  3:111575/1‑135 (MQ=255)
                        aGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCa                                                                                                                         >  8:165366/1‑149 (MQ=255)
                                  aaCAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTg                                                                                                                <  8:136594/148‑1 (MQ=255)
                                    cAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGAGACAAAGTTGCCGCAAATCATCAGGCCAGACCCTCTTGCCACCTTTCAGGGTAGGGC‑TCACCAATTCTGaa                                                                                                              >  3:115887/1‑148 (MQ=255)
                                     aaaaTTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGCCCAGAGCCTCTGGCCACGTTTCAGGGAAGGGC‑TCACCATTTCTGAAc                                                                                                             >  7:44065/1‑148 (MQ=255)
                                         ttCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAg                                                                                                                                     <  8:168111/121‑1 (MQ=255)
                                         ttCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAg                                                                                                                                     >  7:168111/1‑121 (MQ=255)
                                            tACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGGCTCTGGCCtga                                                                                                                                           <  1:214605/112‑3 (MQ=255)
                                            tACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGGCTCTGGCCtga                                                                                                                                           >  2:214605/1‑110 (MQ=255)
                                              cTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCAGCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGGCGCAAATGATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTa                                                                                                      <  8:139848/146‑1 (MQ=255)
                                              cTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTa                                                                                                      <  2:225092/146‑1 (MQ=255)
                                                 ccGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGtt                                                                                                 <  4:174562/148‑1 (MQ=255)
                                                 ccGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTc                                                                                                >  6:295845/1‑149 (MQ=255)
                                                 ccGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTc                                                                                                >  2:247822/1‑149 (MQ=255)
                                                    gCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGAc                                                                                             >  7:235198/1‑149 (MQ=255)
                                                     ccAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGAc                                                                                             <  8:235198/148‑1 (MQ=255)
                                                          aaTAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTc                                                                                       <  2:37339/149‑1 (MQ=255)
                                                          aaTAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTc                                                                                       >  7:43947/1‑149 (MQ=255)
                                                              gTTGAACCGCTTAGTGGGATTTTCACCCTTGGCGGTTTTGCAAGAATCTGCGACTAAGTGGCCGCAAATCATCAGGCCAGAGGCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTTGTGTTCGACGCTTTCGGa                                                                                    <  4:115887/148‑1 (MQ=255)
                                                               ttGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGaca                                                                                  <  5:295845/149‑1 (MQ=255)
                                                                    ccccGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGtt                                                                                                 >  2:268123/1‑129 (MQ=255)
                                                                     cccGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAAc                                                                            >  1:95061/1‑149 (MQ=255)
                                                                     cccGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAAc                                                                            <  8:43947/149‑1 (MQ=255)
                                                                       cGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGc                                                                          >  1:319940/1‑149 (MQ=255)
                                                                           gTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGtt                                                                      <  1:136908/149‑1 (MQ=255)
                                                                            tCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGttt                                                                     >  1:222235/1‑149 (MQ=255)
                                                                                       cccTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACt                                                          >  5:9118/1‑149 (MQ=255)
                                                                                        ccTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCTCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACtt                                                         <  2:95061/149‑1 (MQ=255)
                                                                                             ccGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTAc                                                     <  2:222235/148‑1 (MQ=255)
                                                                                              cGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCg                                                   >  5:87034/1‑149 (MQ=255)
                                                                                                gTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGc                                                  >  4:222616/1‑148 (MQ=255)
                                                                                                   ttGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAg                                               <  3:222616/148‑1 (MQ=255)
                                                                                                   ttGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAg                                               <  2:319940/148‑1 (MQ=255)
                                                                                                        acaaTCTTCGACAAAGTGGCCGCAAATCATGTGGCGAGAGCCTCTGGCATCGTTTCAGGTTAGGGC‑TCAGCATTTCTGAACTTTTGTTCTGTTCGACGCTTTCGGACAGAGATCGCCGTTTGCTGGAGCACTTGTACCGCCAGCACAgg                                         <  8:44065/149‑2 (MQ=255)
                                                                                                         caaTCTGCGACAAATTGTCCGCAAATCATCAGGCCAGAGCCTCTTGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGATCAATTGTACTGTTCGACGCTTTCGGACACAGAACTCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCt                                        <  5:101701/149‑1 (MQ=255)
                                                                                                                  aCAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATgc                               <  6:87034/149‑1 (MQ=255)
                                                                                                                                  tcatcaGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGc               >  5:147429/1‑149 (MQ=255)
                                                                                                                                  aaacgaGGCCAGAGGCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGc               >  2:173190/6‑149 (MQ=255)
                                                                                                                                       aGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAAc          >  1:293494/1‑149 (MQ=255)
                                                                                                                                        ggCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGCGTCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAAc          >  1:220618/1‑149 (MQ=255)
                                                                                                                                        ggCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACt         >  3:318995/1‑149 (MQ=255)
                                                                                                                                           cAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACtgtg      <  2:293494/149‑1 (MQ=255)
                                                                                                                                            agagCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTgg     <  4:318995/149‑1 (MQ=255)
                                                                                                                                            agagCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTgg     <  2:2490/149‑1 (MQ=255)
                                                                                                                                               gCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTa  <  1:247822/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GTACCGATCCCGCTTCGTACTGCCAGGGGGGATGAACAAAATTCTACTCCCGGCCAGCAATAGTTGAACCCCGTAGTCGGATATTCACCCTTGCCGGTTTTGCAACAATCTGCGACAAAGTGGCCGCAAATCATCAGGCCAGAGCCTCTGGCCTCGTTTCAGGGTAGGGC‑TCACCATTTCTGAACAATTGTACTGTTCGACGCTTTCGGACACAGAACGCCGTTTGCTGGACCACTTCTACCGCCAGCACAGCTCACGCATGCGCGCTGCAAGCGACGCCGAACTGTGGGTA  >  NC_002947/5179868‑5180159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑