Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,182,997	T→A	9.2%	K246M (AAG→ATG) ‡	PP_4563 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,182,997	0	T	A	9.2%	182.0 / 8.4	65	K246M (AAG→ATG) ‡	PP_4563	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/30);  new base A (3/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGG  >  NC_002947/5182851‑5183140                                                                                                                                                   |                                                                                                                                                  gTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTg                                                                                                                                                 >  1:132602/1‑149 (MQ=255)  tGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGc                                                                                                                                                <  7:56992/149‑1 (MQ=255)   ggCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAAGAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCt                                                                                                                                               >  1:282435/1‑149 (MQ=255)     cGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                                                                                                             <  5:345910/149‑1 (MQ=255)     cGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGa                                                                                                                                             <  3:252362/149‑1 (MQ=255)          agcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCaa                                                                                                                                         <  7:57472/148‑1 (MQ=255)          agcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAAc                                                                                                                                        <  2:272370/149‑1 (MQ=255)           gcTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACg                                                                                                                                       >  7:21781/1‑149 (MQ=255)                 cTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATTGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGt                                                                                                                                 <  5:2517/149‑1 (MQ=255)                    ccGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAgg                                                                                                                                   <  8:169151/144‑1 (MQ=255)                    ccGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAgg                                                                                                                                   >  7:169151/1‑144 (MQ=255)                    ccGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCAc                                                                                                                              >  1:12718/1‑149 (MQ=255)                      gCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCActc                                                                                                                            <  6:332457/149‑1 (MQ=255)                        aaCTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCtt                                                                                                                          <  2:282435/149‑1 (MQ=255)                             cGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTc                                                                                                                       <  1:4652/147‑1 (MQ=255)                                  aGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACAcc                                                                                                               >  6:17491/1‑149 (MQ=255)                                  aGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACAcc                                                                                                               <  8:21781/149‑1 (MQ=255)                                       tAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCaa                                                                                                          <  7:135729/149‑1 (MQ=255)                                            ccGTCAGGCTTTCTCGATGCGATCGTCATGGATTACGATCAGGCCCTGCTTGAACAACCAGCCAATGGCCTTCTTGAAGTTGCACTTGCTGACGTTGAACAGCTTGCTGATCAACGAAGGGTCACTCTTGTCGC‑ACAACCCCAacacac                                                                                                     >  3:157906/1‑147 (MQ=255)                                              gTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCg                                                                                                   >  8:35066/1‑149 (MQ=255)                                                 aGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCt                                                                                                >  7:313002/1‑149 (MQ=255)                                                    cTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCat                                                                                                          >  7:1188/1‑135 (MQ=255)                                                    cTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCat                                                                                                          <  8:1188/136‑2 (MQ=255)                                                           gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCtt                                                                                         <  5:17491/146‑1 (MQ=255)                                                           gATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCtt                                                                                         <  8:313002/146‑1 (MQ=255)                                                            aTGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑Ag                                                                                    <  2:132602/149‑1 (MQ=255)                                                            aTGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑Ag                                                                                    <  5:45611/149‑1 (MQ=255)                                                              gCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCACCACCCCGCCTTCGGCTTCC‑AGgc                                                                                  >  8:41317/1‑149 (MQ=255)                                                                gATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGgcgc                                                                                <  3:313289/149‑1 (MQ=255)                                                                    gTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGcc                                                                             <  5:81872/148‑1 (MQ=255)                                                                     tCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGc                                                                                                                                                >  2:70071/1‑82 (MQ=255)                                                                     tCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGc                                                                                                                                                <  1:70071/82‑1 (MQ=255)                                                                     tCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCAt                                                                           >  7:22412/1‑149 (MQ=255)                                                                          gATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATc                                                                       <  8:22412/148‑1 (MQ=255)                                                                          gATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCt                                                                      >  4:35744/1‑149 (MQ=255)                                                                                gATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                                                                                                        >  8:227845/1‑79 (MQ=38)                                                                                gATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGATCAGCAAGCTGTTCAAc                                                                                                                                        <  7:227845/79‑1 (MQ=38)                                                                                        ccTGCTTGAACAACCCGCCAATGGCCTTCTTGATGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGAAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGgtg                                                        >  4:53250/1‑149 (MQ=255)                                                                                        ccTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAgg                                                           >  4:290153/1‑146 (MQ=255)                                                                                        ccTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAgg                                                           <  3:290153/146‑1 (MQ=255)                                                                                        ccTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGgtg                                                        >  3:163669/1‑149 (MQ=255)                                                                                               gAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGTTCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGGCTTCGGCTTCCAAGGCGCGCCATGATCTGCTCTTGCAGGGTGTcc                                                     <  1:41846/146‑1 (MQ=255)                                                                                               gAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTcc                                                     >  2:41846/1‑145 (MQ=255)                                                                                               gAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTcc                                                     >  4:164412/1‑145 (MQ=255)                                                                                               gAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTcc                                                     <  3:164412/145‑1 (MQ=255)                                                                                                  caacCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAggg                                              <  7:152127/149‑1 (MQ=255)                                                                                                      ccGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGc                                                   >  4:37135/1‑140 (MQ=255)                                                                                                      ccGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGc                                                   <  3:37135/140‑1 (MQ=255)                                                                                                                tcttcttGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGc                                >  3:115663/2‑149 (MQ=255)                                                                                                                  ttcttGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTg                              >  1:323281/1‑149 (MQ=255)                                                                                                                  ttcttGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACAACCCGCCTTCGGATTAC‑AGGCTCGCCATGATATGCTCTTGCAGGGTGTCCGCATCGGCTGCGCCTACAGGCt                               >  1:227391/1‑148 (MQ=255)                                                                                                                      tGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTAc                                    <  2:54764/139‑1 (MQ=255)                                                                                                                      tGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTAc                                    >  1:54764/1‑139 (MQ=255)                                                                                                                          gTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAgg                                                           <  2:264821/112‑1 (MQ=255)                                                                                                                          gTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAgg                                                           >  1:264821/1‑112 (MQ=255)                                                                                                                           ttGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAg                     >  5:278332/1‑149 (MQ=255)                                                                                                                                    cctgcGTTGATCAGCAAGCTGTTCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCtt            >  4:239915/5‑149 (MQ=255)                                                                                                                                     tGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTg           >  7:122502/1‑149 (MQ=255)                                                                                                                                     ctgcGTTGATCAGCAAGCTGTTCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAgg                          >  3:210065/4‑134 (MQ=255)                                                                                                                                     ctgcGTTGATCAGCAAGCTGTTCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAgg                          <  4:210065/131‑1 (MQ=255)                                                                                                                                       aCGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGcc         <  3:35744/149‑1 (MQ=255)                                                                                                                                          ttGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTc      <  2:12718/149‑1 (MQ=255)                                                                                                                                             aaCAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAgg                                               <  7:6805/105‑1 (MQ=255)                                                                                                                                             aaCAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAgg                                               >  8:6805/1‑105 (MQ=255)                                                                                                                                               cAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGCAACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTATTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTgg  >  1:51125/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GTGGCGAGCAGCTGGACTTCCGCAACTTCGTACAGGCGTAAGGCCGTCAGGCTTTCTCGATGCGATCGTCATGGATGACGATCAGGCCCTGCTTGAACAACCCGCCAATGGCCTTCTTGAAGTTGCCCTTGCTGACGTTGAACAGCTTGCTGATCAACGCAGGGTCACTCTTGTCGC‑ACACCCCCAACACCCCGCCTTCGGCTTCC‑AGGCGCGCCATGATCTGCTCTTGCAGGGTGTCCGCCAGGGCGGCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGG  >  NC_002947/5182851‑5183140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑