Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,019 C→T 7.5% E99K (GAG→AAG)  PP_0354 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,0190CT 7.5% 155.3 / 2.5 53E99K (GAG→AAG) PP_0354CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (27/22);  new base T (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAGAAGC  >  NC_002947/431875‑432165
                                                                                                                                                 |                                                                                                                                                  
gggCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCg                                                                                                                                                >  8:95248/1‑149 (MQ=255)
 ggCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGc                                                                                                                                               >  2:199928/1‑149 (MQ=255)
 ggCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGc                                                                                                                                               >  6:304925/1‑149 (MQ=255)
   cAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTg                                                                                                                                             >  4:230645/1‑149 (MQ=255)
              aaCACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGaa                                                                                                                                  <  3:21277/149‑1 (MQ=255)
                  caTCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCTGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTg                                                                                                                              <  7:45546/149‑1 (MQ=255)
                         cATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGa                                                                                                                       <  7:187638/149‑1 (MQ=255)
                                       ggATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGc                                                                                                          >  2:252548/1‑148 (MQ=255)
                                            aaTGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCTAGGTg                                                                                                    <  7:141905/149‑1 (MQ=255)
                                                  gCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGgcg                                                                                              >  8:173382/1‑149 (MQ=255)
                                                           aGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCGTGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCaa                                                                                      >  1:72164/1‑149 (MQ=255)
                                                             ttGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCa                                                                                   >  1:137504/1‑149 (MQ=255)
                                                                caggcagAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCAcgc                                                                                >  1:150163/1‑149 (MQ=255)
                                                                   gcagAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGc                                                                             <  3:40161/149‑1 (MQ=255)
                                                                          gtgtCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCtt                                                                      >  4:190521/1‑149 (MQ=255)
                                                                                 ccccGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTg                                                                                                            <  5:266623/104‑1 (MQ=255)
                                                                                 ccccGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTg                                                                                                            >  6:266623/1‑104 (MQ=255)
                                                                                   ccGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATg                                                               >  3:252599/1‑147 (MQ=255)
                                                                                   ccGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTa                                                             <  1:199928/149‑1 (MQ=255)
                                                                                      gCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGaa                                                          <  5:304925/149‑1 (MQ=255)
                                                                                      gCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGaa                                                          <  3:230645/149‑1 (MQ=255)
                                                                                       ccAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAg                                                         >  2:155291/1‑149 (MQ=255)
                                                                                            tGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTc                                                    <  7:95248/149‑1 (MQ=255)
                                                                                                 ggTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCg                                               <  2:72164/149‑1 (MQ=255)
                                                                                                            gCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCgg                                      >  8:13376/1‑148 (MQ=255)
                                                                                                              ccGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAg                                   >  4:303118/1‑149 (MQ=255)
                                                                                                              ccGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAg                                   >  2:37054/1‑149 (MQ=255)
                                                                                                              ccGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAg                                   <  7:173382/149‑1 (MQ=255)
                                                                                                               cGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAgg                                  <  2:150163/149‑1 (MQ=255)
                                                                                                                    aCCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGttt                               <  7:13376/147‑1 (MQ=255)
                                                                                                                    aCCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGa                             >  6:166052/1‑149 (MQ=255)
                                                                                                                     cccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGAc                            <  1:155291/149‑1 (MQ=255)
                                                                                                                      ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGccc                                           <  8:91531/133‑1 (MQ=255)
                                                                                                                      ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGccc                                           >  7:91531/1‑133 (MQ=255)
                                                                                                                      ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGAc                            >  5:156617/1‑148 (MQ=255)
                                                                                                                      ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGAc                            >  6:177599/1‑148 (MQ=255)
                                                                                                                      ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGAc                            >  5:114664/1‑148 (MQ=255)
                                                                                                                       cagcagGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGa                          >  3:182360/1‑149 (MQ=255)
                                                                                                                        agcagGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGAt                         >  4:70072/1‑149 (MQ=255)
                                                                                                                               cTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCa                  >  2:224581/1‑149 (MQ=255)
                                                                                                                               cTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCa                  <  3:303118/149‑1 (MQ=255)
                                                                                                                                 accAGCGGGGAGTGCTTCCCGCTGGCGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATg                        >  5:308251/2‑141 (MQ=255)
                                                                                                                                 accAGCGGGGAGTGCTTCCCGCTGGCGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATg                        <  6:308251/140‑1 (MQ=255)
                                                                                                                                  ccAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCAc                 <  5:166052/147‑1 (MQ=255)
                                                                                                                                   cAGCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCg               >  2:277558/1‑148 (MQ=255)
                                                                                                                                    aGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCa             <  6:114664/149‑1 (MQ=255)
                                                                                                                                     gCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGc                                                      <  6:45627/107‑1 (MQ=255)
                                                                                                                                       gggAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGc          <  1:37054/149‑1 (MQ=255)
                                                                                                                                        ggAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCg         >  7:96520/1‑149 (MQ=255)
                                                                                                                                        ggAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCg         >  3:106403/1‑149 (MQ=255)
                                                                                                                                             cACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAGaa    <  3:70072/149‑1 (MQ=255)
                                                                                                                                              aCTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAGAAg   >  7:100825/1‑149 (MQ=255)
                                                                                                                                               cTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAGAAgc  <  5:177599/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTTCCCCGGCCAGGTGCTCGGTGCG‑GGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAGAAGC  >  NC_002947/431875‑432165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑