Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,336,179 G→A 11.6% R870H (CGC→CAC)  PP_4695 → two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,336,1790GA11.6% 111.4 / 4.8 43R870H (CGC→CAC) PP_4695two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base A (2/3);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

GCAGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAA  >  NC_002947/5336032‑5336320
                                                                                                                                                   |                                                                                                                                             
gcAGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAgcgc                                                                                                                                              >  8:308749/1‑149 (MQ=255)
  aGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCt                                                                                                                                            <  8:68681/149‑1 (MQ=255)
    ccaacaGAACCGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCAAAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTgg                                                                                                                                          <  2:166015/149‑1 (MQ=255)
              aGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCt                                                                                                                                <  1:324894/149‑1 (MQ=255)
                cGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCTgc                                                                                                                              <  1:73995/149‑2 (MQ=255)
                  aggagCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCTgcgg                                                                                                                            <  3:144494/149‑4 (MQ=255)
                     agCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAAc                                                                                                                         <  3:40621/149‑1 (MQ=255)
                             tgCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCtctc                                                                                                                 <  7:90744/149‑1 (MQ=255)
                               ccTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTcc                                                                                                                >  1:137901/1‑148 (MQ=255)
                                     tGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCTgcgg                                                                                                                            >  1:346278/1‑127 (MQ=255)
                                     tGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCTgcgg                                                                                                                            <  2:346278/130‑4 (MQ=255)
                                                  gATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTcgcc                                                                                            >  5:281163/1‑149 (MQ=255)
                                                   aTGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTcgccg                                                                                           >  7:276388/1‑149 (MQ=255)
                                                         tCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCgg                                                                                      >  7:168588/1‑148 (MQ=255)
                                                           ggTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCa                                                                                    <  7:308749/148‑1 (MQ=255)
                                                                  tgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGcc                                                                               <  8:85545/146‑1 (MQ=255)
                                                                  tgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGcc                                                                               >  7:85545/1‑146 (MQ=255)
                                                                  tgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtg                                                                            >  8:113926/1‑149 (MQ=255)
                                                                   ggCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgc                                                                           >  3:52752/1‑149 (MQ=255)
                                                                          aaCCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgcgcgtgc                                                                     <  8:276388/148‑1 (MQ=255)
                                                                             cGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAg                                                                 <  6:281163/149‑1 (MQ=255)
                                                                                 gcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAg                                                             <  4:52752/149‑1 (MQ=255)
                                                                                     tttCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGtt                                                          >  7:43047/1‑148 (MQ=255)
                                                                                         gAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGCTGACCCGCAGAGACTGCCCCAGGTCCTGATCAACCTGCTCTCCAACTCCAGCCATGCATCGCCGCACGGCAGCGCCTTGCGCGTGCGCAGCGAGGTTACCGa                                                     >  6:111966/1‑149 (MQ=255)
                                                                                                 gTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgcgc                                                                         >  1:196132/1‑121 (MQ=255)
                                                                                                 gTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgcgc                                                                         <  2:196132/121‑1 (MQ=255)
                                                                                                  ttcttcAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGt                                            >  8:3906/1‑149 (MQ=255)
                                                                                                       cAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGAc                                        <  3:26819/148‑1 (MQ=255)
                                                                                                        aaCCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCt                                      >  2:31437/1‑149 (MQ=255)
                                                                                                                  aCCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGc                                                   >  2:183406/1‑126 (MQ=255)
                                                                                                                  aCCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGc                                                   <  1:183406/126‑1 (MQ=255)
                                                                                                                    ccGGACCATTGGGCCGATGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                          <  1:136114/149‑1 (MQ=255)
                                                                                                                    ccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                          <  8:168588/149‑1 (MQ=255)
                                                                                                                    ccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                          <  8:43047/149‑1 (MQ=255)
                                                                                                                    ccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                          >  7:57298/1‑149 (MQ=255)
                                                                                                                       gACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACg                        >  8:160400/1‑148 (MQ=255)
                                                                                                                              gggCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTAGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGc                >  8:86182/1‑149 (MQ=255)
                                                                                                                               ggCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCg               <  4:299240/149‑1 (MQ=255)
                                                                                                                                 ccGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCgg              >  5:117909/1‑148 (MQ=255)
                                                                                                                                       gTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCg       <  6:117909/149‑1 (MQ=255)
                                                                                                                                            ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGc                                                   <  2:324029/100‑1 (MQ=255)
                                                                                                                                            ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGc                                                   >  1:324029/1‑100 (MQ=255)
                                                                                                                                            ccGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaagaa  >  6:42021/1‑149 (MQ=255)
                                                                                                                                             atcAGCACCTGGGCCAGGCGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaagaa  >  2:286004/3‑148 (MQ=255)
                                                                                                                                                  cgcCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGa                             >  2:72590/1‑116 (MQ=255)
                                                                                                                                                  cgcCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGa                             <  1:72590/116‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GCAGCCACCAGAACAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAA  >  NC_002947/5336032‑5336320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑