Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,341,505 C→T 6.4% A204A (GCC→GCT)  panB → 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,341,5050CT 6.4% 184.2 / 2.2 63A204A (GCC→GCT) panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base T (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CCAGGAGGCCCAGGCCCGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGC  >  NC_002947/5341358‑5341653
                                                                                                                                                   |                                                                                                                                                     
ccAGGAGGCCCAGGCCCGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCg                                                                                                                                                      <  2:267814/149‑1 (MQ=255)
         ccaggcccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCa                                                                                                                                             >  2:146699/1‑149 (MQ=255)
          caggcccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCTGCCGGCGCCaa                                                                                                                                            <  6:13888/149‑2 (MQ=255)
          caggcccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCAc                                                                                                                                            >  7:276880/1‑149 (MQ=255)
              cccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTATTCGGCATTGGCGCCGGCAGCGCCACCGAt                                                                                                                                        <  4:281864/149‑1 (MQ=255)
                cGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATgg                                                                                                                                      <  8:276880/149‑1 (MQ=255)
                 ggCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGc                                                                                                                                     >  2:191748/1‑149 (MQ=255)
                        gcgcgcCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtac                                                                                                                              >  6:311362/1‑149 (MQ=255)
                            gcCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtactggt                                                                                                                          <  6:125105/149‑1 (MQ=255)
                              cGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtactggtac                                                                                                                        >  4:87177/1‑149 (MQ=255)
                               gACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtactggtact                                                                                                                       >  2:290196/1‑149 (MQ=255)
                                  gCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCTGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCa                                                                                                                    <  3:220511/149‑1 (MQ=255)
                                   ccATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCAc                                                                                                                   >  4:38429/1‑149 (MQ=255)
                                    cATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCAc                                                                                                                   >  1:146635/1‑148 (MQ=255)
                                     aTTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGa                                                                                                                 >  8:208743/1‑149 (MQ=255)
                                          ccTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATg                                                                                                             >  4:132920/1‑148 (MQ=255)
                                          ccTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGc                                                                                                            <  5:139336/149‑1 (MQ=255)
                                           cTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCt                                                                                                           >  2:254314/1‑149 (MQ=255)
                                              gAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTc                                                                                                          <  2:250943/147‑1 (MQ=255)
                                              gAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCg                                                                                                         <  3:87177/148‑1 (MQ=255)
                                              gAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCg                                                                                                         <  2:146635/148‑1 (MQ=255)
                                               aaCAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGc                                                                                                       <  1:191748/149‑1 (MQ=255)
                                               aaCAGGCCGGTGCGGACATGCTGCTGCTCGAGTGCGTACCCATCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCTGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGc                                                                                                       <  7:128789/149‑1 (MQ=255)
                                                 cAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCt                                                                                                     >  3:236796/1‑149 (MQ=255)
                                                    gCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑c                                                                                                 <  6:188455/149‑1 (MQ=255)
                                                    gCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑c                                                                                                 <  4:50872/149‑1 (MQ=255)
                                                        gTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCt                                                                                              >  1:163868/1‑148 (MQ=255)
                                                              ccATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGc                                                                                       <  5:122068/149‑1 (MQ=255)
                                                                 tgctgctgcTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCgg                                                                                    <  8:112243/149‑1 (MQ=255)
                                                                 tgctgctgcTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCgg                                                                                    <  1:290196/149‑1 (MQ=255)
                                                                     gctgctCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTAc                                                                                >  4:216374/1‑149 (MQ=255)
                                                                      ctgctCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTa                                                                                 <  3:132920/147‑1 (MQ=255)
                                                                        gctCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCg                                                                              >  1:189104/1‑148 (MQ=255)
                                                                            gAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTTGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGtt                                                                         >  2:190273/1‑149 (MQ=255)
                                                                               tGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      <  1:146699/149‑1 (MQ=255)
                                                                               tGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      <  4:236796/149‑1 (MQ=255)
                                                                                      ccAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAAc                                                               >  5:140151/1‑149 (MQ=255)
                                                                                         gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                            <  7:208743/149‑1 (MQ=255)
                                                                                         gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                            <  3:38429/149‑1 (MQ=255)
                                                                                          cGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcg                                                           <  3:216374/149‑2 (MQ=255)
                                                                                                   ggcggAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCAGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGc                                                   >  8:170554/1‑148 (MQ=255)
                                                                                                          aTCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                           >  6:133844/1‑149 (MQ=255)
                                                                                                            cACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                         >  8:230158/1‑149 (MQ=255)
                                                                                                             aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTAc                                                                                >  5:35718/1‑109 (MQ=255)
                                                                                                             aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTAc                                                                                <  6:35718/109‑1 (MQ=255)
                                                                                                             aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGatat                                        >  1:146835/1‑149 (MQ=255)
                                                                                                              ccAATTCCGTTGGCATCCCGGTAATCGGCATTGTCGCAGGCAACGCCACAGAAGTCAAGGTACTAGTTCTAAACGACATTAACGGACTGT‑CGCTTAGCTGCAGCGAACAGAAGTTCGTGCAGAACGACATGCTCGGCCAGCCGGagaga                                       >  4:174047/1‑145 (MQ=255)
                                                                                                               cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                       >  6:92805/1‑148 (MQ=255)
                                                                                                                  tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTATCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                   <  1:72534/149‑1 (MQ=255)
                                                                                                                  tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                   <  2:189104/149‑1 (MQ=255)
                                                                                                                    ccGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAg                                  <  2:163868/148‑1 (MQ=255)
                                                                                                                          gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtc                           >  5:311356/1‑149 (MQ=255)
                                                                                                                                cGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCt                     >  7:47768/1‑149 (MQ=255)
                                                                                                                                   tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                       >  5:35596/1‑128 (MQ=255)
                                                                                                                                   tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                       <  6:35596/128‑1 (MQ=255)
                                                                                                                                       cGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      >  4:230356/1‑93 (MQ=255)
                                                                                                                                       cGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      <  3:230356/93‑1 (MQ=255)
                                                                                                                                        ggCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCg              <  8:47768/148‑1 (MQ=255)
                                                                                                                                        atcggtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGAGATCCATAGCGCCCTCGTCGCTTACGTc               >  2:14587/6‑147 (MQ=255)
                                                                                                                                            gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtcg                       >  7:192792/2‑135 (MQ=255)
                                                                                                                                            gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtcg                       <  8:192792/134‑1 (MQ=255)
                                                                                                                                               gcgcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTAAGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCACCCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGt       >  1:267841/1‑149 (MQ=255)
                                                                                                                                                 gcCGGCAGCGCCACCGATGGCCAGGTACTGTTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGTTACCGAAGTTCGTGAATAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa    <  5:311362/149‑1 (MQ=255)
                                                                                                                                                   cGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc  <  6:140151/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCAGGAGGCCCAGGCCCGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGC  >  NC_002947/5341358‑5341653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑