Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,341,507 G→C 6.3% G205A (GGC→GCC)  panB → 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,341,5070GC 6.3% 199.2 / 4.2 63G205A (GGC→GCC) panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

CCAGGCCCGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGC  >  NC_002947/5341367‑5341653
                                                                                                                                            |                                                                                                                                                   
ccaggcccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCa                                                                                                                                             >  2:146699/1‑149 (MQ=255)
 caggcccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCTGCCGGCGCCaa                                                                                                                                            <  6:13888/149‑2 (MQ=255)
 caggcccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCAc                                                                                                                                            >  7:276880/1‑149 (MQ=255)
     cccGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTATTCGGCATTGGCGCCGGCAGCGCCACCGAt                                                                                                                                        <  4:281864/149‑1 (MQ=255)
       cGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATgg                                                                                                                                      <  8:276880/149‑1 (MQ=255)
        ggCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGc                                                                                                                                     >  2:191748/1‑149 (MQ=255)
               gcgcgcCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtac                                                                                                                              >  6:311362/1‑149 (MQ=255)
                   gcCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtactggt                                                                                                                          <  6:125105/149‑1 (MQ=255)
                     cGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtactggtac                                                                                                                        >  4:87177/1‑149 (MQ=255)
                      gACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAggtactggtact                                                                                                                       >  2:290196/1‑149 (MQ=255)
                         gCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCTGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCa                                                                                                                    <  3:220511/149‑1 (MQ=255)
                          ccATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCAc                                                                                                                   >  4:38429/1‑149 (MQ=255)
                           cATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCAc                                                                                                                   >  1:146635/1‑148 (MQ=255)
                            aTTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGa                                                                                                                 >  8:208743/1‑149 (MQ=255)
                                 ccTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATg                                                                                                             >  4:132920/1‑148 (MQ=255)
                                 ccTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGc                                                                                                            <  5:139336/149‑1 (MQ=255)
                                  cTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCt                                                                                                           >  2:254314/1‑149 (MQ=255)
                                     gAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTc                                                                                                          <  2:250943/147‑1 (MQ=255)
                                     gAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCg                                                                                                         <  3:87177/148‑1 (MQ=255)
                                     gAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCg                                                                                                         <  2:146635/148‑1 (MQ=255)
                                      aaCAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGc                                                                                                       <  1:191748/149‑1 (MQ=255)
                                      aaCAGGCCGGTGCGGACATGCTGCTGCTCGAGTGCGTACCCATCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCTGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGc                                                                                                       <  7:128789/149‑1 (MQ=255)
                                        cAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCt                                                                                                     >  3:236796/1‑149 (MQ=255)
                                           gCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑c                                                                                                 <  6:188455/149‑1 (MQ=255)
                                           gCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑c                                                                                                 <  4:50872/149‑1 (MQ=255)
                                               gTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCt                                                                                              >  1:163868/1‑148 (MQ=255)
                                                     ccATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGc                                                                                       <  5:122068/149‑1 (MQ=255)
                                                        tgctgctgcTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCgg                                                                                    <  8:112243/149‑1 (MQ=255)
                                                        tgctgctgcTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCgg                                                                                    <  1:290196/149‑1 (MQ=255)
                                                            gctgctCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTAc                                                                                >  4:216374/1‑149 (MQ=255)
                                                             ctgctCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTa                                                                                 <  3:132920/147‑1 (MQ=255)
                                                               gctCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCg                                                                              >  1:189104/1‑148 (MQ=255)
                                                                   gAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTTGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGtt                                                                         >  2:190273/1‑149 (MQ=255)
                                                                      tGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      <  1:146699/149‑1 (MQ=255)
                                                                      tGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      <  4:236796/149‑1 (MQ=255)
                                                                             ccAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAAc                                                               >  5:140151/1‑149 (MQ=255)
                                                                                gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                            <  7:208743/149‑1 (MQ=255)
                                                                                gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                            <  3:38429/149‑1 (MQ=255)
                                                                                 cGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcg                                                           <  3:216374/149‑2 (MQ=255)
                                                                                          ggcggAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCAGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGc                                                   >  8:170554/1‑148 (MQ=255)
                                                                                                 aTCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                           >  6:133844/1‑149 (MQ=255)
                                                                                                   cACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                         >  8:230158/1‑149 (MQ=255)
                                                                                                    aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTAc                                                                                >  5:35718/1‑109 (MQ=255)
                                                                                                    aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTAc                                                                                <  6:35718/109‑1 (MQ=255)
                                                                                                    aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGatat                                        >  1:146835/1‑149 (MQ=255)
                                                                                                     ccAATTCCGTTGGCATCCCGGTAATCGGCATTGTCGCAGGCAACGCCACAGAAGTCAAGGTACTAGTTCTAAACGACATTAACGGACTGT‑CGCTTAGCTGCAGCGAACAGAAGTTCGTGCAGAACGACATGCTCGGCCAGCCGGagaga                                       >  4:174047/1‑145 (MQ=255)
                                                                                                      cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                       >  6:92805/1‑148 (MQ=255)
                                                                                                         tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTATCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                   <  1:72534/149‑1 (MQ=255)
                                                                                                         tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                   <  2:189104/149‑1 (MQ=255)
                                                                                                           ccGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAg                                  <  2:163868/148‑1 (MQ=255)
                                                                                                                 gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtc                           >  5:311356/1‑149 (MQ=255)
                                                                                                                       cGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCt                     >  7:47768/1‑149 (MQ=255)
                                                                                                                          tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                       >  5:35596/1‑128 (MQ=255)
                                                                                                                          tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                       <  6:35596/128‑1 (MQ=255)
                                                                                                                              cGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      >  4:230356/1‑93 (MQ=255)
                                                                                                                              cGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                      <  3:230356/93‑1 (MQ=255)
                                                                                                                               ggCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCg              <  8:47768/148‑1 (MQ=255)
                                                                                                                               atcggtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGAGATCCATAGCGCCCTCGTCGCTTACGTc               >  2:14587/6‑147 (MQ=255)
                                                                                                                                   gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtcg                       >  7:192792/2‑135 (MQ=255)
                                                                                                                                   gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtcg                       <  8:192792/134‑1 (MQ=255)
                                                                                                                                      gcgcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTAAGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCACCCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGt       >  1:267841/1‑149 (MQ=255)
                                                                                                                                        gcCGGCAGCGCCACCGATGGCCAGGTACTGTTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGTTACCGAAGTTCGTGAATAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa    <  5:311362/149‑1 (MQ=255)
                                                                                                                                          cGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc  <  6:140151/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CCAGGCCCGGCAGATGCGCGCCGACGCCATTGCCCTGGAACAGGCCGGTGCGGCCATGCTGCTGCTCGAGTGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGC  >  NC_002947/5341367‑5341653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑