Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,341,584	G→T	5.3%	V231L (GTG→TTG) ‡	panB →	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,341,584	0	G	T	5.3%	243.9 / 3.3	76	V231L (GTG→TTG) ‡	panB	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base T (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

TGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCG  >  NC_002947/5341437‑5341726                                                                                                                                                     |                                                                                                                                                  tGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                                                                                                  <  1:146699/149‑1 (MQ=255) tGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                                                                                                  <  4:236796/149‑1 (MQ=255)        ccAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAAc                                                                                                                                           >  5:140151/1‑149 (MQ=255)           gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                                                                                                        <  7:208743/149‑1 (MQ=255)           gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                                                                                                        <  3:38429/149‑1 (MQ=255)            cGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcg                                                                                                                                       <  3:216374/149‑2 (MQ=255)                     ggcggAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCAGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGc                                                                                                                               >  8:170554/1‑148 (MQ=255)                            aTCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                                                                                                       >  6:133844/1‑149 (MQ=255)                              cACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                                                                                                     >  8:230158/1‑149 (MQ=255)                               aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGatat                                                                                                                    >  1:146835/1‑149 (MQ=255)                                ccAATTCCGTTGGCATCCCGGTAATCGGCATTGTCGCAGGCAACGCCACAGAAGTCAAGGTACTAGTTCTAAACGACATTAACGGACTGT‑CGCTTAGCTGCAGCGAACAGAAGTTCGTGCAGAACGACATGCTCGGCCAGCCGGagaga                                                                                                                   >  4:174047/1‑145 (MQ=255)                                 cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                                                                                                   >  6:92805/1‑148 (MQ=255)                                    tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTATCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                                                                                               <  1:72534/149‑1 (MQ=255)                                    tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                                                                                               <  2:189104/149‑1 (MQ=255)                                      ccGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAg                                                                                                              <  2:163868/148‑1 (MQ=255)                                            gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtc                                                                                                       >  5:311356/1‑149 (MQ=255)                                                  cGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCt                                                                                                >  7:47768/1‑149 (MQ=255)                                                     tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                                                                                                   >  5:35596/1‑128 (MQ=255)                                                     tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                                                                                                   <  6:35596/128‑1 (MQ=255)                                                         cGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                                                                                                  <  3:230356/93‑1 (MQ=255)                                                         cGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                                                                                                  >  4:230356/1‑93 (MQ=255)                                                          ggCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCg                                                                                         <  8:47768/148‑1 (MQ=255)                                                          atcggtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGAGATCCATAGCGCCCTCGTC‑GCTTACGTc                                                                                          >  2:14587/6‑147 (MQ=255)                                                              gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc‑g                                                                                                  >  7:192792/2‑135 (MQ=255)                                                              gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc‑g                                                                                                  <  8:192792/134‑1 (MQ=255)                                                                 gcgcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTAAGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCACCCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGt                                                                                  >  1:267841/1‑149 (MQ=255)                                                                   gcCGGCAGCGCCACCGATGGCCAGGTACTGTTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGTTACCGAAGTTCGTGAATAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaa                                                                               <  5:311362/149‑1 (MQ=255)                                                                     cGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagc                                                                             <  6:140151/149‑1 (MQ=255)                                                                            gcCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAg                                                                      >  1:109859/1‑149 (MQ=255)                                                                              cACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGtt                                                                    >  5:20933/1‑149 (MQ=255)                                                                                 cGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCAAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                 >  5:274654/1‑149 (MQ=255)                                                                                  gATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTccc                                                                <  5:92805/149‑1 (MQ=255)                                                                                  gATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                 >  7:220776/1‑148 (MQ=255)                                                                                    tGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAg                                                              <  6:311356/149‑1 (MQ=255)                                                                                      gCCAGGTAGTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGCCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCCAGGCGGTCAAGCAAGTCAGTTTccagg                                                              >  2:81512/1‑144 (MQ=255)                                                                                      gCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGc                                                            >  2:264401/1‑149 (MQ=255)                                                                                        cAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAg                                                          >  5:349707/1‑149 (MQ=255)                                                                                         aGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑c                                                        >  7:276406/1‑149 (MQ=255)                                                                                          ggtactggtactGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑c                                                        >  4:124848/1‑148 (MQ=255)                                                                                                 gtactgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑Aacac                                                 <  1:254314/148‑1 (MQ=255)                                                                                                 gtactgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACgg                                               <  2:267841/149‑1 (MQ=255)                                                                                                   actgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGt                                             >  6:57580/1‑149 (MQ=255)                                                                                                    ctgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGtt                                            >  1:236980/1‑149 (MQ=255)                                                                                                    ctgCACGACATGCTCGGCCTGT‑CGCTGAGCAGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGtt                                            <  7:170554/149‑1 (MQ=255)                                                                                                      gCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGtt                                            >  3:120445/1‑147 (MQ=255)                                                                                                       cACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           <  5:24313/117‑1 (MQ=255)                                                                                                       cACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           >  6:24313/1‑117 (MQ=255)                                                                                                        aCGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGt                                        <  7:230158/149‑1 (MQ=255)                                                                                                          gACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGc                                      <  6:20933/149‑1 (MQ=255)                                                                                                          gACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACAGGATATCCATAGCGCCCTCGTA‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTg                                       >  2:258503/1‑148 (MQ=255)                                                                                                           aCATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCa                                     <  6:349707/149‑1 (MQ=255)                                                                                                            cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                             <  1:209918/126‑1 (MQ=255)                                                                                                            cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                             >  2:209918/1‑126 (MQ=255)                                                                                                            cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCAt                                    >  3:336177/1‑149 (MQ=255)                                                                                                            cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCAt                                    <  4:26715/149‑1 (MQ=255)                                                                                                             aTGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATg                                   <  3:124848/149‑1 (MQ=255)                                                                                                               gCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                    >  6:96396/1‑85 (MQ=255)                                                                                                               gCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                    <  5:96396/85‑1 (MQ=255)                                                                                                                    gCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCTGTCAAGCAAGTCAGTTTCCCAGGCAGACG‑AACACGGGTTCAGTGCATGAATACa                             <  1:14587/149‑1 (MQ=255)                                                                                                                      cTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          <  8:220776/149‑1 (MQ=255)                                                                                                                       tGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          >  3:136755/1‑148 (MQ=255)                                                                                                                         t‑CGCTGAGCGGCCGTGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAg                       <  5:133844/149‑1 (MQ=255)                                                                                                                         t‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCaa                        <  2:109859/148‑1 (MQ=255)                                                                                                                           cGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           <  6:264821/98‑1 (MQ=255)                                                                                                                           cGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           >  5:264821/1‑98 (MQ=255)                                                                                                                            gCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGt                                        <  6:172324/130‑1 (MQ=255)                                                                                                                            gCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGt                                        >  5:172324/1‑130 (MQ=255)                                                                                                                                      cGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGaa           <  5:57580/149‑1 (MQ=255)                                                                                                                                       gggTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTGCGCGAAc          >  4:205071/1‑149 (MQ=255)                                                                                                                                         tgcatgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          >  5:264152/6‑131 (MQ=255)                                                                                                                                         tgcatgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          <  6:264152/126‑1 (MQ=255)                                                                                                                                            atgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgc     >  6:214762/3‑149 (MQ=255)                                                                                                                                              gAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAg                            <  6:292653/124‑1 (MQ=255)                                                                                                                                              gAAGTTCGTGAAGAACTTCAAGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAg                            >  5:292653/1‑124 (MQ=255)                                                                                                                                               aaGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CGCAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgcgc   <  1:258503/149‑1 (MQ=255)                                                                                                                                               aaGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGcgcgcg  >  3:71066/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TGCGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCG  >  NC_002947/5341437‑5341726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑