Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,341,586 G→C 5.6% V231V (GTG→GTC) ‡ panB → 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,341,5860GC 5.6% 224.3 / 4.0 72V231V (GTG→GTC) ‡panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

CCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCG  >  NC_002947/5341444‑5341726
                                                                                                                                               |                                                                                                                                               
ccAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAAc                                                                                                                                           >  5:140151/1‑149 (MQ=255)
   gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                                                                                                        <  7:208743/149‑1 (MQ=255)
   gCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTc                                                                                                                                        <  3:38429/149‑1 (MQ=255)
    cGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcg                                                                                                                                       <  3:216374/149‑2 (MQ=255)
             ggcggAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCAGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGc                                                                                                                               >  8:170554/1‑148 (MQ=255)
                    aTCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                                                                                                       >  6:133844/1‑149 (MQ=255)
                      cACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGata                                                                                                                     >  8:230158/1‑149 (MQ=255)
                       aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGatat                                                                                                                    >  1:146835/1‑149 (MQ=255)
                        ccAATTCCGTTGGCATCCCGGTAATCGGCATTGTCGCAGGCAACGCCACAGAAGTCAAGGTACTAGTTCTAAACGACATTAACGGACTGT‑CGCTTAGCTGCAGCGAACAGAAGTTCGTGCAGAACGACATGCTCGGCCAGCCGGagaga                                                                                                                   >  4:174047/1‑145 (MQ=255)
                         cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                                                                                                   >  6:92805/1‑148 (MQ=255)
                            tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTATCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                                                                                               <  1:72534/149‑1 (MQ=255)
                            tGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATa                                                                                                               <  2:189104/149‑1 (MQ=255)
                              ccGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAg                                                                                                              <  2:163868/148‑1 (MQ=255)
                                    gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtc                                                                                                       >  5:311356/1‑149 (MQ=255)
                                          cGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCt                                                                                                >  7:47768/1‑149 (MQ=255)
                                             tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                                                                                                   <  6:35596/128‑1 (MQ=255)
                                             tAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATc                                                                                                                   >  5:35596/1‑128 (MQ=255)
                                                  ggCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCg                                                                                         <  8:47768/148‑1 (MQ=255)
                                                  atcggtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGAGATCCATAGCGCCCTCGTC‑GCTTACGTc                                                                                          >  2:14587/6‑147 (MQ=255)
                                                      gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc‑g                                                                                                  >  7:192792/2‑135 (MQ=255)
                                                      gtGGCGCTGCCGGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc‑g                                                                                                  <  8:192792/134‑1 (MQ=255)
                                                         gcgcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTAAGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCACCCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGt                                                                                  >  1:267841/1‑149 (MQ=255)
                                                           gcCGGCAGCGCCACCGATGGCCAGGTACTGTTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGTTACCGAAGTTCGTGAATAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaa                                                                               <  5:311362/149‑1 (MQ=255)
                                                             cGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagc                                                                             <  6:140151/149‑1 (MQ=255)
                                                                    gcCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAg                                                                      >  1:109859/1‑149 (MQ=255)
                                                                      cACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGtt                                                                    >  5:20933/1‑149 (MQ=255)
                                                                         cGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCAAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                 >  5:274654/1‑149 (MQ=255)
                                                                          gATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTccc                                                                <  5:92805/149‑1 (MQ=255)
                                                                          gATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                 >  7:220776/1‑148 (MQ=255)
                                                                            tGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAg                                                              <  6:311356/149‑1 (MQ=255)
                                                                              gCCAGGTAGTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGCCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCCAGGCGGTCAAGCAAGTCAGTTTccagg                                                              >  2:81512/1‑144 (MQ=255)
                                                                              gCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGc                                                            >  2:264401/1‑149 (MQ=255)
                                                                                cAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAg                                                          >  5:349707/1‑149 (MQ=255)
                                                                                 aGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑c                                                        >  7:276406/1‑149 (MQ=255)
                                                                                  ggtactggtactGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑c                                                        >  4:124848/1‑148 (MQ=255)
                                                                                         gtactgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑Aacac                                                 <  1:254314/148‑1 (MQ=255)
                                                                                         gtactgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACgg                                               <  2:267841/149‑1 (MQ=255)
                                                                                           actgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGt                                             >  6:57580/1‑149 (MQ=255)
                                                                                            ctgCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGtt                                            >  1:236980/1‑149 (MQ=255)
                                                                                            ctgCACGACATGCTCGGCCTGT‑CGCTGAGCAGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGtt                                            <  7:170554/149‑1 (MQ=255)
                                                                                              gCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGtt                                            >  3:120445/1‑147 (MQ=255)
                                                                                               cACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           >  6:24313/1‑117 (MQ=255)
                                                                                               cACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           <  5:24313/117‑1 (MQ=255)
                                                                                                aCGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGt                                        <  7:230158/149‑1 (MQ=255)
                                                                                                  gACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGc                                      <  6:20933/149‑1 (MQ=255)
                                                                                                  gACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACAGGATATCCATAGCGCCCTCGTA‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTg                                       >  2:258503/1‑148 (MQ=255)
                                                                                                   aCATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCa                                     <  6:349707/149‑1 (MQ=255)
                                                                                                    cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                             >  2:209918/1‑126 (MQ=255)
                                                                                                    cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                             <  1:209918/126‑1 (MQ=255)
                                                                                                    cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCAt                                    >  3:336177/1‑149 (MQ=255)
                                                                                                    cATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCAt                                    <  4:26715/149‑1 (MQ=255)
                                                                                                     aTGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATg                                   <  3:124848/149‑1 (MQ=255)
                                                                                                       gCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                    <  5:96396/85‑1 (MQ=255)
                                                                                                       gCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                    >  6:96396/1‑85 (MQ=255)
                                                                                                            gCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCTGTCAAGCAAGTCAGTTTCCCAGGCAGACG‑AACACGGGTTCAGTGCATGAATACa                             <  1:14587/149‑1 (MQ=255)
                                                                                                              cTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          <  8:220776/149‑1 (MQ=255)
                                                                                                               tGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          >  3:136755/1‑148 (MQ=255)
                                                                                                                 t‑CGCTGAGCGGCCGTGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAg                       <  5:133844/149‑1 (MQ=255)
                                                                                                                 t‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCaa                        <  2:109859/148‑1 (MQ=255)
                                                                                                                   cGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           <  6:264821/98‑1 (MQ=255)
                                                                                                                   cGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTcaagcaa                                                                           >  5:264821/1‑98 (MQ=255)
                                                                                                                    gCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGt                                        <  6:172324/130‑1 (MQ=255)
                                                                                                                    gCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGt                                        >  5:172324/1‑130 (MQ=255)
                                                                                                                              cGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGaa           <  5:57580/149‑1 (MQ=255)
                                                                                                                               gggTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTGCGCGAAc          >  4:205071/1‑149 (MQ=255)
                                                                                                                                 tgcatgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          >  5:264152/6‑131 (MQ=255)
                                                                                                                                 tgcatgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTc                          <  6:264152/126‑1 (MQ=255)
                                                                                                                                    atgAAGTTCTTCACGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgc     >  6:214762/3‑149 (MQ=255)
                                                                                                                                      gAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAg                            <  6:292653/124‑1 (MQ=255)
                                                                                                                                      gAAGTTCGTGAAGAACTTCAAGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAg                            >  5:292653/1‑124 (MQ=255)
                                                                                                                                       aaGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CGCAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgcgc   <  1:258503/149‑1 (MQ=255)
                                                                                                                                       aaGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGcgcgcg  >  3:71066/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGT‑CGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTC‑GCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAG‑CG‑AACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCG  >  NC_002947/5341444‑5341726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑