Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,342,571 T→G 7.4% intergenic (+17/‑190) panC → / → pgi‑II pantothenate synthetase/glucose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,342,5710TG 7.4% 153.6 / 2.6 51intergenic (+17/‑190)panC/pgi‑IIpantothenate synthetase/glucose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (25/22);  new base G (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

ATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGA  >  NC_002947/5342430‑5342715
                                                                                                                                             |                                                                                                                                                 
aTGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGcc                                                                                                                                             >  5:339497/1‑148 (MQ=255)
   ccGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAt                                                                                                                                          <  8:194113/148‑1 (MQ=255)
    cGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTc                                                                                                                                        >  5:53647/1‑149 (MQ=255)
        aGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTcgcg                                                                                                                                     <  6:339497/148‑1 (MQ=255)
           ttGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGc                                                                                                                                                 <  7:269868/133‑1 (MQ=255)
           ttGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGc                                                                                                                                                 >  8:269868/1‑133 (MQ=255)
              cgcCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGc                                                                                                                              <  6:245723/149‑1 (MQ=255)
               gcCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTg                                                                                                                                                  >  1:136875/1‑128 (MQ=255)
               gcCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGcttgcc                                                                                                                             >  8:114221/1‑144 (MQ=255)
               gcCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGcc                                                                                                                             >  7:287725/1‑149 (MQ=255)
                   ggCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGATGCCTTGCAGCACATTCGCGGGCAAGCCC‑GCt                                                                                                                        >  8:306745/1‑149 (MQ=255)
                     cAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTcc                                                                                                                      <  1:315063/149‑1 (MQ=255)
                      aGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCt                                                                                                                     <  3:183519/149‑1 (MQ=255)
                              aTGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGtt                                                                                                              >  3:323580/1‑148 (MQ=255)
                                     agaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCAAGGCAGCCCaagacc                                                                                                                                      <  3:112063/118‑6 (MQ=255)
                                     agaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCAAGGCAGCCCaagacc                                                                                                                                      >  4:112063/1‑113 (MQ=255)
                                      gaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCt                                                                                                                        >  2:292843/1‑130 (MQ=255)
                                      gaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCt                                                                                                                        <  1:292843/130‑1 (MQ=255)
                                           ggtggtGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGgggcg                                                                                                >  5:186930/1‑149 (MQ=255)
                                            gtggtgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGgggcgg                                                                                               <  6:53647/149‑1 (MQ=255)
                                            ggcgtgATTGCGGCGGCATACCTGCGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCCCCTACAGGTAAACGTGCAGgagcg                                                                                                <  8:190892/145‑1 (MQ=38)
                                                tgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGgctt                                                                                           <  8:287725/149‑1 (MQ=255)
                                                 gATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGgcttg                                                                                          >  7:297380/1‑149 (MQ=255)
                                                     gcggcgGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGgcttgctt                                                                                       <  8:297380/148‑1 (MQ=255)
                                                      cggcggCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCttgcttgc                                                                                     <  4:323580/149‑1 (MQ=255)
                                                      cggcggCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCttgcttgc                                                                                     <  7:306745/149‑1 (MQ=255)
                                                        gcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCaa                                                                                   >  6:101554/1‑149 (MQ=255)
                                                                     gTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAACACCCCATAACGGTCTTGCGCTGCCTTGCAGCCCAATCGCCGCAAAGCCC‑GCTCCTGCACGTATACCTGAAGGGGCGCGCTTGATTACAAAGCCCCCaaaaa                                                                       >  8:222158/1‑147 (MQ=255)
                                                                            gcgGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACTGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGc                                                               <  5:101554/149‑1 (MQ=255)
                                                                                  gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCCAAATACCCCAGCCATAcc                                                         >  3:252207/1‑149 (MQ=255)
                                                                                    tCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCCTAcccc                                                       >  7:192917/1‑148 (MQ=255)
                                                                                      gACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCAtt                                                     >  7:70868/1‑149 (MQ=255)
                                                                                       aCAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTAGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAAAGCCCCCAAAATACCCCAGCCATACCCAtta                                                    >  2:87622/1‑148 (MQ=255)
                                                                                        cAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTcc                                                   <  8:70868/149‑1 (MQ=255)
                                                                                                   tGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGc                                        >  3:95068/1‑149 (MQ=255)
                                                                                                    gCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGGCCCCAAAATCCCCCAGCCCTACCCATTCCCCGCCCGTGGcc                                       >  5:136172/1‑149 (MQ=39)
                                                                                                    gCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGcc                                       >  7:95720/1‑149 (MQ=255)
                                                                                                       tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCttt                                    <  1:87622/149‑1 (MQ=255)
                                                                                                          ggAAGAGAAGACCTCATAACGGTCTTGGGCTGCCTTGCAGCGCATTCGCGGGCAAGCCC‑GCTACTGCTCGTTTACCTGTAGGGGAGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCGATACCCATTCCCAGCACGTGGCCTTTGc                                  <  7:222158/148‑1 (MQ=255)
                                                                                                           gaagagaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCt                                <  6:186930/149‑1 (MQ=255)
                                                                                                                    aCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAAt                           <  1:174408/145‑1 (MQ=255)
                                                                                                                         aTAACGGTCTCGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCCGCACGTGGCCTTTGCCTATAATGGTGCCAg                   >  1:70775/1‑149 (MQ=255)
                                                                                                                            aCGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCt                <  8:192917/148‑1 (MQ=255)
                                                                                                                            aCGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCt                <  8:95720/148‑1 (MQ=255)
                                                                                                                                gaatGGGCTGCAAGGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCt                >  2:87679/4‑144 (MQ=255)
                                                                                                                                gaatGGGCTGCAAGGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCt                <  1:87679/141‑1 (MQ=255)
                                                                                                                                     ggCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGc       >  5:293142/1‑148 (MQ=255)
                                                                                                                                     ggCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGc       >  4:16207/1‑148 (MQ=255)
                                                                                                                                      gCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGc                  >  8:141315/1‑136 (MQ=255)
                                                                                                                                      gCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGc                  <  7:141315/136‑1 (MQ=255)
                                                                                                                                         gCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGa  >  5:35146/1‑149 (MQ=255)
                                                                                                                                            ttGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGt                        >  7:306375/1‑124 (MQ=255)
                                                                                                                                            ttGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGt                        <  8:306375/124‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
ATGCCGTAAGCTTGCGCCCGGCAGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCC‑GCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGA  >  NC_002947/5342430‑5342715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑