Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,373,246	C→T	9.1%	noncoding (80/82 nt)	PP_mr55 →	P31 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,373,246	0	C	T	9.1%	176.5 / 5.4	65	noncoding (80/82 nt)	PP_mr55	P31 ncRNA
Reads supporting (aligned to +/- strand):  ref base C (31/28);  new base T (2/4);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

CGTCGGCACCGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACATCTGTGTTGGCT  >  NC_002947/5373100‑5373389                                                                                                                                                   |                                                                                                                                                 cGTCGGCACCGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCActcc                                                                                                                                                <  3:226249/149‑1 (MQ=255)     ggCACCGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCACTCCGCtt                                                                                                                                            <  7:220505/149‑1 (MQ=39)      gCACCGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCttt                                                                                                                                           >  2:24441/1‑149 (MQ=255)         ccGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGGAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCAt                                                                                                                                        <  6:71911/149‑1 (MQ=255)         ccGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCACTCCGCtttttt                                                                                                                                        <  1:296160/149‑4 (MQ=38)                 ttCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCACTCCGCtttttt                                                                                                                                        >  8:42628/1‑138 (MQ=38)                 ttCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCACTCCGCtttttt                                                                                                                                        <  7:42628/141‑4 (MQ=38)                 ttCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGaa                                                                                                                                >  7:288909/1‑149 (MQ=255)                   cACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGaat                                                                                                                               <  5:13109/148‑1 (MQ=255)                    aCCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGaatt                                                                                                                              >  3:176683/1‑148 (MQ=255)                      cgcgGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGaattc‑aa                                                                                                                          >  4:130581/1‑149 (MQ=255)                      cgcgGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGaattc‑aa                                                                                                                          >  1:183486/1‑149 (MQ=255)                        cgGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTTaattc‑aatt                                                                                                                        <  1:24441/149‑1 (MQ=255)                          gAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCg                                                                                                                      >  3:152104/1‑149 (MQ=255)                           aaaaTACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGc                                                                                                                     >  1:53891/1‑149 (MQ=255)                                  tGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCACTCCGCtttttt                                                                                                                                        <  4:98532/124‑4 (MQ=37)                                  tGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGGGTGACGTGTCCATACGTCACTCCGCtttttt                                                                                                                                        >  3:98532/1‑121 (MQ=37)                                  tGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTgcgcgc                                                                                                              >  7:200274/1‑149 (MQ=255)                                         ggCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAg                                                                                                        <  8:288909/148‑1 (MQ=255)                                              gggCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGaca                                                                                                  <  2:53891/149‑1 (MQ=255)                                                     tATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTg                                                                                                      <  4:96113/138‑1 (MQ=255)                                                     tATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTg                                                                                                      >  3:96113/1‑138 (MQ=255)                                                          ctggctTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTCAAATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTg                                                                                       <  1:235923/149‑1 (MQ=255)                                                          ctggctTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGacac                                                                                                 <  7:160880/138‑1 (MQ=255)                                                          ctggctTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGacac                                                                                                 >  8:160880/1‑138 (MQ=255)                                                             gctTTGTCAAGAAGACCTACCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGc                                                                                   <  8:205583/149‑1 (MQ=255)                                                                     aagaagACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGt                                                                                                       <  3:129798/121‑1 (MQ=255)                                                                     aagaagACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGt                                                                                                       >  4:129798/1‑121 (MQ=255)                                                                     aagaagACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGaca                                                                                                  >  6:64183/1‑126 (MQ=255)                                                                     aagaagACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGaca                                                                                                  <  5:64183/126‑1 (MQ=255)                                                                     aagaagACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGa                                                                            >  5:235908/1‑148 (MQ=255)                                                                       gaagaCCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGaaaa                                                                         <  2:183486/149‑1 (MQ=255)                                                                             cTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGGTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAgggtgg                                                                   >  8:124960/1‑148 (MQ=255)                                                                             cTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAgtgtgt                                                                   >  8:145637/1‑149 (MQ=255)                                                                             cTCCAGTAAATAAAGCATGAAAGGGAGATCGCAGCTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCACGCTTTAATGTGCTCAATTC‑AATTCGCTGCGCGATTTTAGAGGACACACTAAATCTGAATCTTACAGAAAAgtgatt                                                                   >  8:150186/1‑146 (MQ=255)                                                                              tCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGtt                                                                  >  1:158527/1‑149 (MQ=255)                                                                              tCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGtt                                                                  >  3:175902/1‑149 (MQ=255)                                                                                ccGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAgtgtgt                                                                   <  4:87311/146‑1 (MQ=255)                                                                                ccGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAgtgtgt                                                                   >  3:87311/1‑146 (MQ=255)                                                                                ccGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGtttt                                                                <  5:95900/149‑1 (MQ=255)                                                                                    aaaTCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGt                                                            >  5:345867/1‑149 (MQ=255)                                                                                      aTCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGaaaa                                                                         <  4:288737/134‑1 (MQ=255)                                                                                      aTCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGaaaa                                                                         >  3:288737/1‑134 (MQ=255)                                                                                       tCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTa                                                         >  6:149863/1‑149 (MQ=255)                                                                                         aaGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTAcc                                                       >  5:316885/1‑149 (MQ=255)                                                                                         aaGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTAc                                                        <  8:249640/148‑1 (MQ=255)                                                                                                aaaGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGAt                                                 <  2:158527/148‑1 (MQ=255)                                                                                                    ggCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGtc                                            >  2:346717/1‑149 (MQ=255)                                                                                                     gCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGtct                                           <  6:235908/149‑1 (MQ=255)                                                                                                      cctgggCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGtctc                                          <  2:271881/144‑1 (MQ=255)                                                                                                        aTCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTctct                                         >  1:38394/1‑148 (MQ=255)                                                                                                         tCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGGTTTCGGTCTACCGCGGATGTGTGTCTa                                        >  6:266141/1‑148 (MQ=255)                                                                                                            cAGGTTGCAAAAAAGCGTAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTTTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAac                                    <  4:176683/149‑1 (MQ=255)                                                                                                               gTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAAcaca                                 <  6:345867/149‑1 (MQ=255)                                                                                                                ttGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACAt                                <  3:130581/149‑1 (MQ=255)                                                                                                                   cAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCaa                             >  2:290429/1‑149 (MQ=255)                                                                                                                     aaaaaGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATg                           >  4:230310/1‑149 (MQ=255)                                                                                                                                  aCGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACa              <  4:175902/149‑1 (MQ=255)                                                                                                                                          acacGTCACCCCTCTTTCATTTTCTTAATTC‑AATTCGCTTCTCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACATCTgtgt       <  7:150186/148‑1 (MQ=255)                                                                                                                                          acacGTCACCCCTCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACATCTgtgt       <  7:145637/148‑1 (MQ=255)                                                                                                                                          acacGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGa                        >  2:80600/1‑131 (MQ=255)                                                                                                                                          acacGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGa                        <  1:80600/131‑1 (MQ=255)                                                                                                                                          acacGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACATCTGTGtt      <  1:346717/149‑1 (MQ=255)                                                                                                                                               tCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACATCTGTGTTGGCt  >  6:28846/1‑148 (MQ=255)                                                                                                                                                 aCCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGAc                       <  1:123411/125‑1 (MQ=255)                                                                                                                                                 aCCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGAc                       >  2:123411/1‑125 (MQ=255)                                                                                                                                                   cccGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGAc                   >  6:337013/1‑127 (MQ=255)                                                                                                                                                   cccGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGAc                   <  5:337013/127‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGTCGGCACCGATGGCTTCACCGCGGAAAATACTGCCGTCGGCAAGGGCGAGTATGGCTGGCTTTGTCAAGAAGACCTCCCGTAAATCAAGCATGAAAGGGCGATCGCAGGTTGCAAAAAAGCGGAGTGACGTATGGACACGTCACCCCGCTTTCATGTGCTGAATTC‑AATTCGCTGCGCGCTTTTAGTGGACACACTAAAGCTGTAGCTTACAGAAAAGTGTGTTTTCGGTCTACCGCGGATGTGTCTCTAAAACACATCAATGCGACAGACTGACATCTGTGTTGGCT  >  NC_002947/5373100‑5373389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑