Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,388,882 C→A 7.8% L798M (CTG→ATG) ‡ dld2 → D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,388,8820CA 7.8% 302.7 / 11.0 97L798M (CTG→ATG) ‡dld2D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (40/49);  new base A (3/4);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

CAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGG  >  NC_002947/5388734‑5389024
                                                                                                                                                     |                                                                                                                                               
cAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGcc                                                                                                                                                 <  3:337857/149‑1 (MQ=255)
       tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGc                                                                                                                                          <  2:176260/148‑1 (MQ=255)
       tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCa                                                                                                                                         <  5:183457/149‑1 (MQ=255)
       tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCa                                                                                                                                         <  8:203194/149‑1 (MQ=255)
         ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAgg                                                                                                                                       >  7:127985/1‑149 (MQ=255)
         ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAg                                                                                                                                        <  7:174946/148‑1 (MQ=255)
         ctgaGGGCAACCGTTCGCCTCCTAGGGCTACCCCGAGCAGGCCGAACACA‑AAGGCCAGGAGCTGATCACCGCCCTGCTGAACGCCAGACGCGGCTGCCTCGACCCGATCAATTCCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAg                                                                                                                                        <  4:14077/148‑1 (MQ=255)
           gcGGCCAACCGTTCGCCTCCAAGGGCTGCCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGa                                                                                                                                      >  6:52828/1‑148 (MQ=255)
           gcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGa                                                                                                                                      <  5:52828/148‑1 (MQ=255)
            cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGa                                                                                                                                      <  7:102985/147‑1 (MQ=255)
            cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGAc                                                                                                                                     <  7:11761/148‑1 (MQ=255)
            cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggg                                                                                                                                      <  5:245607/147‑2 (MQ=255)
             ggCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggggct                                                                                                                                   <  3:5795/149‑5 (MQ=255)
               ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCt                                                                                                                                   >  8:2929/1‑147 (MQ=255)
                 aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGc                                                                                                                               >  1:120101/1‑149 (MQ=255)
                 aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggg                                                                                                                                      >  7:290552/1‑141 (MQ=255)
                 aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggg                                                                                                                                      <  8:290552/142‑2 (MQ=255)
                  aCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGc                                                                                                                               >  2:200437/1‑148 (MQ=255)
                   ccGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGa                                                                                                                             <  3:3320/149‑1 (MQ=255)
                     gTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACCCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGAGCAGGACCTGAGCGaca                                                                                                                           >  1:44391/1‑149 (MQ=255)
                      ttCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGacac                                                                                                                          >  5:27832/1‑149 (MQ=255)
                         gCCTCCAAGGGCTACCCCGAGCAGGCCGAACACATACCGCCAGGAGCTGATCACCCCCCTGCTCCACGCCAGCCGCGGCGGCCTCGACCCGAGCTATTGCGACACAAGCCTCTGCACCCTGCGCC‑Tg                                                                                                                                              >  1:46148/1‑127 (MQ=255)
                         gCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCTCTGCACCCTGCGCC‑Tg                                                                                                                                              <  2:46148/126‑1 (MQ=255)
                          ccTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCgg                                                                                                                      >  4:208645/1‑149 (MQ=255)
                            tCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                    >  8:166957/1‑149 (MQ=255)
                            tCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACCCCAGCCGCGCCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                    >  1:320721/1‑149 (MQ=255)
                             ccAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggggc                                                                                                                                    >  7:163602/1‑129 (MQ=255)
                             ccAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggggc                                                                                                                                    <  8:163602/132‑4 (MQ=255)
                              cAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTgg                                                                                                                  <  3:208645/149‑1 (MQ=255)
                              cAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                   >  5:142738/1‑148 (MQ=255)
                               aaGGGCTACCCCGAGCAGGCCGAACACA‑ACCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACACGGCTGGa                                                                                                                 >  4:117356/1‑149 (MQ=255)
                               aaGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                 <  2:104189/149‑1 (MQ=255)
                                aGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                <  6:27832/149‑1 (MQ=255)
                                   gCTACCCCGAGCATGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGTCTGGACCTg                                                                                                             <  8:47007/149‑1 (MQ=255)
                                   gCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTg                                                                                                             <  2:120101/149‑1 (MQ=255)
                                       ccccGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTAc                                                                                                          <  7:2929/148‑1 (MQ=255)
                                          cGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcc                                                                                                      <  2:67334/149‑1 (MQ=255)
                                            aGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                    <  8:127985/149‑1 (MQ=255)
                                               aGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                 <  7:166957/149‑1 (MQ=255)
                                                 gCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTTCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGCCTGGACCTGTACGACCCCGTgcg                                                                                               >  7:195406/1‑149 (MQ=255)
                                                 gCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCGCCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCg                                                                                                   <  1:265571/145‑1 (MQ=255)
                                                 gCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCGCCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCg                                                                                                   >  2:265571/1‑145 (MQ=255)
                                                  ccGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                              <  3:140689/149‑1 (MQ=255)
                                                   cGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTCTACGACCCCGTGCGCt                                                                                             >  3:233578/1‑149 (MQ=255)
                                                        aca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGa                                                                                                                                 >  8:136372/1‑108 (MQ=255)
                                                        aca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGa                                                                                                                                 <  7:136372/108‑1 (MQ=255)
                                                        aca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                        >  6:210481/1‑149 (MQ=255)
                                                         ca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATcc                                                                                       <  2:44391/149‑1 (MQ=255)
                                                          a‑aaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                      >  5:263666/1‑149 (MQ=255)
                                                          a‑aaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                      >  1:277945/1‑149 (MQ=255)
                                                             aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                    <  5:332650/149‑1 (MQ=255)
                                                             aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                    >  6:235798/1‑149 (MQ=255)
                                                                ccAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacc                                                                                  >  2:107803/1‑148 (MQ=255)
                                                                  acgAGCTGAGCACCGCCCGGCTGCACGCCAGCCGCGGCGGCCTGGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTTCGCTTCATCCGcacccac                                                                               <  4:233578/147‑1 (MQ=255)
                                                                  aGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                               <  7:110506/149‑1 (MQ=255)
                                                                   ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccacc                                                                              >  7:131695/1‑149 (MQ=255)
                                                                   ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccacc                                                                              >  6:243860/1‑149 (MQ=255)
                                                                    gAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                             <  1:18163/149‑1 (MQ=255)
                                                                       cTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                    <  8:34244/123‑1 (MQ=255)
                                                                       cTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                    >  7:34244/1‑123 (MQ=255)
                                                                         gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                          <  7:41891/131‑1 (MQ=255)
                                                                         gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                          >  8:41891/1‑131 (MQ=255)
                                                                            cACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                     >  6:138905/1‑101 (MQ=255)
                                                                            cACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                     <  5:138905/101‑1 (MQ=255)
                                                                               cGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgaccgac                                                                  <  2:9403/149‑1 (MQ=255)
                                                                                gCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACt                                                                 <  3:117356/149‑1 (MQ=255)
                                                                                 cccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                               >  1:266324/1‑134 (MQ=255)
                                                                                 cccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                               <  2:266324/134‑1 (MQ=255)
                                                                                   ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                              <  1:200437/149‑1 (MQ=255)
                                                                                   ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                              <  2:239199/149‑1 (MQ=255)
                                                                                          aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                             >  8:318012/1‑127 (MQ=255)
                                                                                          aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                             <  7:318012/127‑1 (MQ=255)
                                                                                          aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                       >  8:82533/1‑149 (MQ=255)
                                                                                          aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                       <  6:142738/149‑1 (MQ=255)
                                                                                            gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcccc                                                     >  2:55454/1‑149 (MQ=255)
                                                                                                gCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAg                                                 <  2:277945/149‑1 (MQ=255)
                                                                                                 ccGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAgg                                                <  8:49731/149‑1 (MQ=255)
                                                                                                  cgcgGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGa                                               <  3:244626/149‑1 (MQ=255)
                                                                                                   gcggcgGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGAc                                              >  6:135677/1‑149 (MQ=255)
                                                                                                    cggcggCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                <  7:175226/130‑1 (MQ=255)
                                                                                                    cggcggCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                >  8:175226/1‑130 (MQ=255)
                                                                                                      gcggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGaa                                           <  5:135677/149‑1 (MQ=255)
                                                                                                       cggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                          <  6:263666/149‑1 (MQ=255)
                                                                                                        ggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAcc                                         <  7:82533/149‑1 (MQ=255)
                                                                                                                 ccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCT‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                                <  5:328024/149‑1 (MQ=255)
                                                                                                                   gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                               >  1:52010/1‑149 (MQ=255)
                                                                                                                    aTCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                             >  1:7610/1‑149 (MQ=255)
                                                                                                                       tATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                          >  1:52898/1‑149 (MQ=255)
                                                                                                                         ttGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                               <  5:235798/142‑1 (MQ=255)
                                                                                                                            cGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGACCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAcc                      <  2:320721/148‑1 (MQ=255)
                                                                                                                                acaAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGCCCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcag                 >  7:321275/1‑149 (MQ=255)
                                                                                                                                 tcaggtccTGCACCAGGCGCA‑GGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACAGGTAGCTGTGCACGTGACCTgcag                 >  2:179793/7‑148 (MQ=255)
                                                                                                                                  aaGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTa                                                                                                           >  2:114134/1‑57 (MQ=255)
                                                                                                                                  aaGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTa                                                                                                           <  1:114134/57‑1 (MQ=255)
                                                                                                                                    gCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacc             <  2:52898/149‑1 (MQ=255)
                                                                                                                                    gCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACACCCCAGGACGAACCGGAAGCTGTGCCCGTGACCTGcagcacc             >  6:4249/1‑149 (MQ=255)
                                                                                                                                               cTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg  <  8:131695/149‑1 (MQ=255)
                                                                                                                                               cTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg  >  5:87219/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CAGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGG  >  NC_002947/5388734‑5389024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑